Role of prostaglandin E2 in basal and noradrenaline-induced progesterone secretion by the bovine corpus luteum

Kotwica, J.; Skarzynski, Dariusz J.; Mlynarczuk, Jarosław; Rękawiecki, Robert

doi:10.1016/s0090-6980(02)00149-1

Cited by 33 publications

(28 citation statements)

References 22 publications

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“…Additionally, Lukasik et al (2010) showed that PGE 2 upregulated P 4 secretion by equine luteal steroidogenic cells in vitro. Furthermore, the stimulatory effect of PGE 2 on P 4 production by steroidogenic cells was also confirmed in the cow in vitro (Kotwica et al 2003).…”

Section: Figurementioning

confidence: 59%

Ovarian steroids affect prostaglandin production in equine endometrial cells in vitro

Szóstek

Galvão

Ferreira‐Dias

et al. 2014

Journal of Endocrinology

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This study aimed to evaluate the influence of ovarian steroids on equine endometrial epithelial and stromal cells, specifically i) prostaglandin (PG) production in a time-dependent manner, ii) specific PG synthases mRNA transcription and protein expression, and iii) cell proliferation. After passage I, cells were exposed to vehicle, oxytocin (OT, positive control, 10 K7 M), progesterone (P 4 , 10 K7 M), 17b estradiol (E 2 , 10 K9 M), or P 4 CE 2 for 12, 24, 48, or 72 h. Following treatment, PG concentration was determined using the direct enzyme immunoassay (EIA) method. Alterations in PG synthases mRNA transcriptions, PG synthases protein expression, and cell proliferation in response to the treatments were determined after 24 h using real-time PCR, western blot, or 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide respectively. After 24 h, E 2 and P 4 CE 2 increased PGE 2 and PGF 2a secretion as well as specific prostaglandinendoperoxide synthase-2 (PTGS2), PGE 2 synthases (PGES), and PGF 2a synthases (PGFS) expression in the epithelial cells (P!0.05). Additionally, E 2 and P 4 CE 2 increased PTGS2 expression in stromal cells after 24 h (P!0.05). In stromal cells, P 4 CE 2 increased PGE 2 production as well as PGES expression after 24 h (P!0.05). Both E 2 and P 4 CE 2 increased PGF 2a production by stromal cells after 24 h (P!0.05). Ovarian steroids affected proliferation of stromal and epithelial cells during the 24-h incubation period (P!0.05). We provide evidence that ovarian steroids affect PG production in equine endometrial cells, upregulating PTGS2, PGES, and PGFS expression. Ovarian steroid-stimulated PG production could be an important mechanism occurring in the equine endometrium that is involved in the regulation of the estrous cycle and early pregnancy.

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Section: Figurementioning

confidence: 59%

Ovarian steroids affect prostaglandin production in equine endometrial cells in vitro

Szóstek

Galvão

Ferreira‐Dias

et al. 2014

Journal of Endocrinology

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“…Prostaglandin F 2a is the major luteolytic agent, whereas PGE 2 has luteoprotective and anti-luteolytic properties (Asselin et al 1996, McCracken et al 1999. Therefore, achieving an optimal PGF 2a to PGE 2 ratio is essential for endometrial receptivity, maintenance of corpus luteum (CL), and P4 secretion as well as accurate pregnancy establishment (Pratt et al 1977, Magness et al 1981, Milvae et al 1996, Kotwica et al 2003, Weems et al 2006. Taking into consideration, the interactions between LPA and PG action and the mechanisms of LPA synthesis (Shah & Catt 2005), we assume that LPA could have a direct role in endometrial secretory functions and signal transduction between embryos and endometrium, not only in mice (Ye et al 2005) and human (Hinokio et al 2002, Dey et al 2004, Liu & Armant 2004, but also in cattle.…”

Section: Introductionmentioning

confidence: 99%

Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus

Wocławek-Potocka¹,

Komiyama²,

Saulnier-Blache³

et al. 2009

Reproduction

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Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor 3 (LPAR3) in the murine endometrium. The lack of functional LPAR3 in mice may lead to embryo mortality. In the present study, we examined the role of LPA in the bovine uterus. We confirmed that LPA is locally produced and released from the bovine endometrium. Moreover, there are enzymes involved in LPA synthesis (phospholipase (PL) D 2 and PLA2G1B) in the bovine endometrium during estrous cycle and early pregnancy. Expression of the receptor for LPA (LPAR1) was positively correlated with the expression of PGE 2 synthase (PGES) and negatively correlated with the expression of PGF 2a synthase (aldose reductase with 20 a-hydroxysteroid dehydrogenase activity -PGFS) during early pregnancy. In vivo LPA induced P4 and PGE 2 secretion was inhibited by LPAR1 antagonist (Ki16425). The overall results indicate that LPA is locally produced and released from the bovine endometrium. Moreover, LPAR1 gene expression in the endometrium during the estrous cycle and early pregnancy indicates that LPA may play autocrine and/or paracrine roles in the bovine uterus. LPAR1 gene expression is positively correlated with the expression of the enzyme responsible for luteotropic PGE 2 production (PGES) in endometrium. In cow, LPA stimulates P4 and PGE 2 secretion. Thus, LPA in the bovine reproductive tract may indirectly (via endometrium) or directly support corpus luteum action via the increase of P4 synthesis and the increase of PGE 2 /PGF 2a ratio. It suggests that LPA may serve as an important factor in the maintenance of early pregnancy in cow. Reproduction (2009) 137 95-105

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“…PGE 2 is luteotrophic and luteoprotective (Pratt et al 1977, Magness et al 1981, Kotwica et al 2003, Figure 3 Concentrations of progesterone (A), PGE 2 (B), and PGFM (C) in peripheral blood plasma of cows infused with saline (gray bars) and various doses of IL1A (lines). All reagents were infused into the uterus.…”

Section: Discussionmentioning

confidence: 99%

“…Uterine and ovarian PGs are considered important factors for the regulation of reproductive events such as ovulation, luteolysis, embryo implantation, and maintenance of pregnancy (Weems et al 2006). Generally, PGE 2 exerts luteotrophic and luteoprotective actions to lengthen the lifespan of CL and sustain progesterone (P 4 ) production (Weems et al 1997, Kotwica et al 2003. PGF 2a is the main luteolytic agent in ruminants (McCracken et al 1999.…”

Section: Introductionmentioning

confidence: 99%

Is interleukin-1α a luteotrophic or luteolytic agent in cattle?

et al. 2010

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Cytokines are thought to regulate prostaglandin (PG) secretion in the bovine endometrium. However, there is no consensus about the role of interleukin-1a (IL1A) on PG secretion. The objective of this study was to examine the influence of IL1A on basal and interferon-t (IFNT)-regulated PG in vitro secretion, as well its effects on PG secretion, progesterone (P 4 ) output, and corpus luteum (CL) in vivo lifespan. Explants of bovine endometrium (days 16-17 of the estrous cycle or early pregnancy) were stimulated with IL1A (10 ng/ml), IFNT (30 ng/ml), or IL1A combined with IFN. IL1A alone strongly stimulated luteotrophic PGE 2 secretion by endometrial tissues of both pregnant and nonpregnant cows. IL1A also stimulated luteolytic PGF 2a output in the late luteal phase. IFNT augmented the stimulatory effect of IL1A on PGE 2 secretion. In an in vivo experiment, saline or IL1A at different doses (0.001-10 mg/per animal) was applied to the uterine lumen on day 16 of the cycle. Only the highest dose of IL1A caused a temporal increase in PGF 2a secretion, while it had no effect on P 4 secretion or CL lifespan. Application of 0.1 and 1 mg IL1A stimulated P 4 and PGE 2 output and prolonged the CL lifespan. Although IL1A may stimulate in vitro luteolytic PGF 2a secretion during the estrous cycle, it only acts as a luteotrophic factor in vivo. IL1A increased luteotrophic PGE 2 and P 4 output, inhibiting spontaneous luteolysis. These luteotrophic effects may result in appropriate luteal development and function in cows during the estrous cycle and early pregnancy.

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Role of prostaglandin E2 in basal and noradrenaline-induced progesterone secretion by the bovine corpus luteum

Cited by 33 publications

References 22 publications

Ovarian steroids affect prostaglandin production in equine endometrial cells in vitro

Ovarian steroids affect prostaglandin production in equine endometrial cells in vitro

Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus

Is interleukin-1α a luteotrophic or luteolytic agent in cattle?

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