Role of prostaglandins in lipopolysaccharide effects on K+‐induced contractions in rabbit small intestine

Rebollar, E.; Guerrero‐Lindner, E.; Arruebo, M. P.; Plaza, Miguel Ángel; Murillo, María Divina

doi:10.1046/j.0001-6772.2003.01189.x

Cited by 9 publications

(8 citation statements)

References 42 publications

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“…The frequency of contractions was expressed as the number of contractions per minute (cpm) in a 5-min period, and the tone of spontaneous contractions as the mean of the tension values recorded in a 5-min period. Amplitude, frequency and tone of spontaneous contractions in the presence of drugs are expressed as a percentage of the values recorded in the absence of drugs (control period) (8,22,23). The responses of longitudinal smooth muscle to KCl were measured as the integrated mechanical activity per second (milinewtons per second, mN s -1 ), and normalized per square millimeter of cross-sectional area (CSA, mm 2 ).…”

Section: Methodsmentioning

confidence: 99%

K+ channels involved in contractility of rabbit small intestine

Lamarca

Grasa

Fagundes

et al. 2006

J. Physiol. Biochem.

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Most excitable cells, including gastrointestinal smooth muscle cells, express several types of K+ channels. The aim of this study was to examine the types of K' channels involved in the contractility of longitudinal smooth muscle of rabbit small intestine in vitro. Spontaneous contractions and KCl-stimulated contractions were reduced by atropine, phentolamine, propranolol, suramin, tetrodotoxin and indomethacin. The amplitude and tone of spontaneous contractions were increased by apamin, charybdotoxin, iberiotoxin, E4031, tetraetylammonium (TEA) and BaCl2. The frequency of contractions was reduced in the presence of apamin and TEA and increased by charybdotoxin. It was found that 4-aminopyridine increased the tone of spontaneous contractions and reduced the amplitude and frequency of contractions. Glibenclamide did not modify the amplitude, frequency or tone of contractions. KCl-stimulated contractions were increased by E4031, were not modified by apamin, glibenclamide, NS1619 or diazoxide, and were reduced by charybdotoxin, TEA, 4-aminopyridine or BaCl2. These results suggest that both Ca2+-activated K+ channels of small and high conductance, and HERG K+ channels and inward rectifier K+ channels participate in spontaneous contractions of small intestine. On the other hand, voltage-dependent K+ channels, HERG K+ channels, inward rectifier K+ channels and high conductance Ca2+-activated K+ channels are involved in KCl-stimulated contractions.

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Section: Methodsmentioning

confidence: 99%

K+ channels involved in contractility of rabbit small intestine

Lamarca

Grasa

Fagundes

et al. 2006

J. Physiol. Biochem.

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“…Previous studies by our group have reported an inhibitory effect of LPS on the ACh‐evoked contractions in two conditions: in rabbits treated with LPS and in intestinal segments incubated with LPS in vitro 4 . The effects of LPS on motility have been attributed to a number of active substances such as prostaglandins and nitric oxide 4,6–8 …”

Section: Introductionmentioning

confidence: 96%

“…4 The effects of LPS on motility have been attributed to a number of active substances such as prostaglandins and nitric oxide. 4,[6][7][8] Lipopolysaccharide induces the generation of potent proinflammatory mediators 9,10 and the release of reactive oxygen species (ROS). 11,12 Oxidative stress is involved in a wide range of pathologies, including cancer, ischaemia/reperfusion, inflammatory bowel disease, neurodegenerative diseases and sepsis.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of p38 MAPK improves intestinal disturbances and oxidative stress induced in a rabbit endotoxemia model

Gonzálo

Grasa

Arruebo

et al. 2009

Neurogastroenterology &Amp; Motility

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“…The ileum from rabbits treated with LPS showed signs of inflammation characterised by slight multifocal oedema in the submucosa, infiltration of some heterophils and lymphocytes in the lamina propria, and the presence of mucus and sloughed epithelial cells in the intestinal lumen ( Figures 1E, F). These results indicate that LPS from E. coli O127:B8 induces sepsis and intestinal inflammation in rabbits, in contrast to E. coli O111:B4 (0.2 µg/kg, 90 min, IV), which induces an increase in the rectal temperature but does not induce intestinal inflammation in rabbits (Rebollar et al, 2002(Rebollar et al, , 2003.…”

Section: Effects Of Lps From E Coli O127:b8 On Rectal Temperature Anmentioning

confidence: 76%

The lipopolysaccharide from Escherichia coli O127:B8 induces inflammation and motility disturbances in rabbit ileum

et al. 2017

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The aim of this work was to evaluate the effects of lipopolysaccharide (LPS) from Escherichia coli O127:B8 on the expression of toll-like receptor 4 (TLR4), the histology, and motor function in rabbit ileum. Rabbits were injected intravenously with saline or LPS (100 μg/kg, 2 h). The mRNA expression and localization of TLR4 were determined by reverse transcriptase-PCR and immunofluorescence, respectively. Histological damage induced by LPS was evaluated in sections of ileum stained with haematoxylin and eosin. Contractility studies of ileum were performed in an organ bath. The mRNA expression of TLR4 decreased in the muscular but not in the mucosal layer of rabbits treated with LPS. TLR4 was localised in both the mucosal and muscular layers of rabbit ileum. LPS induced intestinal inflammation and altered the spontaneous contractions and the serotonin-, acetylcholine- and KCl-induced contractions. In conclusion, LPS from E. coli O127:B8 induced a decrease in the mRNA expression of TLR4, an inflammatory response, and changes in the contractility of rabbit ileum.

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Role of prostaglandins in lipopolysaccharide effects on K⁺‐induced contractions in rabbit small intestine

Abstract: These results suggest that effect of LPS on K+-induced contractions in the rabbit small bowel may be mediated by prostaglandin E2.

Cited by 9 publications

References 42 publications

K+ channels involved in contractility of rabbit small intestine

K+ channels involved in contractility of rabbit small intestine

Inhibition of p38 MAPK improves intestinal disturbances and oxidative stress induced in a rabbit endotoxemia model

The lipopolysaccharide from Escherichia coli O127:B8 induces inflammation and motility disturbances in rabbit ileum

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