Role of Proteases in Photo-aging of the Skin

Ghosh, Rita

doi:10.1007/978-981-10-2513-6_20

Cited by 3 publications

(4 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MMPs contribute to the degradation of the extracellular matrix (ECM), signi cant amongst them are MMP-1, -3 and − 9 that have a direct correlation with photoaging [2]. UVA irradiation induces activation of transcription factors like AP-1 and NF-κB, which are involved in the transcriptional regulation of MMPs [14].…”

Section: Resultsmentioning

confidence: 99%

“…Several genes have been associated with chronological aging of the skin that includes various stress reaction genes, anti-oxidative genes, suppressor genes and DNA repair genes. UVA radiation initiates a large range of signal transduction transcription factors to modulate the photoaging process [2]. UVR activates various matrix metallopeptidases (MMPs), which causes alterations in the proteins of the extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

“…MMPs play a pivotal role in photoaging, photocarcinogenesis as well as tumour metastasis [3]. Of the 28 different MMPs found in human cells, MMP-1, -3, and − 9, have been implicated as the key regulators during photoaging of the skin by degradation of the dermal ECM [2]. UVA directly induces MMP-1 activation that has been involved in the degradation of type-I collagen.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Role of Bystander Effect in Ultraviolet A Induced Photoaging

Hansda

Ghosh

2022

Cell Biochem Biophys

View full text Add to dashboard Cite

Background: Exposure to sunlight, mainly UVA, leads to typical changes in the features of the skin known as photoaging. UVA irradiation induces the expression of proteases that are responsible for the degradation of the extracellular matrix proteins to results in photoaging; it also downregulates the expression of proteins that are needed for the skin structure. Since, it is known that cells in the neighborhood of irradiated cells, but not directly exposed to it, often manifest responses like their irradiated counterparts, it is important to evaluate if these bystander cells too, can contribute to photoaging.Methods and Results: UVA induced cell cycle arrest have been associated with photoaging, from ow cytometry analysis we found that there was an induction of cell cycle arrest at the G 1 /S phase in the UVAbystander cells. The expression of some key photoaging marker genes likes, matrix metalloproteinases (MMP-1, MMP-3, MMP-9), cyclooxygenase-2 (COX-2), collagen1 and elastin were assessed from qRT-PCR.Upregulation of MMP-1 and COX-2, downregulation of collagen1 and elastin, along with suppression below normal expression for MMP-3 and MMP-9 was observed in the UVA-bystander A375 cells. Conclusion: Our ndings for the rst time suggest the contribution of UVA-bystander cells to the process of photoaging. HighlightsG 1 /S cell cycle arrest was observed in UVA-bystander A375 cells.The expression of MMP-1 & COX-2 was upregulated in UVA-bystander A375 cells.Collagen 1 & elastin expression was also downregulated in these bystander cells.Expression of MMP-3 & -9 in the UVA-bystander cells lower than that in control cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Role of Bystander Effect in Ultraviolet A Induced Photoaging

Hansda

Ghosh

2022

Cell Biochem Biophys

View full text Add to dashboard Cite

show abstract

“…We have therefore observed the distribution of cells in the different phases at various times (0, 6, 12 and 24 h) after exposure to UVA-CM to assess the in uence on cell cycle progression in the UVA-bystander A375 cells. The ndings are shown in The MMPs contribute to the degradation of the extracellular matrix (ECM), signi cant amongst them are MMP-1, -3 and − 9 that have a direct correlation with photoaging [2]. UVA irradiation induces activation of transcription factors like AP-1 and NF-κB, which are involved in the transcriptional regulation of MMPs [14].…”

Section: Resultsmentioning

confidence: 99%

The Role of Bystander Effect in Ultraviolet A Induced Photoaging

Hansda

Ghosh

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Exposure to sunlight, mainly UVA, leads to typical changes in the features of the skin known as photoaging. UVA irradiation induces the expression of proteases that are responsible for the degradation of the extracellular matrix proteins to results in photoaging; it also downregulates the expression of proteins that are needed for the skin structure. Since, it is known that cells in the neighborhood of irradiated cells, but not directly exposed to it, often manifest responses like their irradiated counterparts, it is important to evaluate if these bystander cells too, can contribute to photoaging. Methods and Results: UVA induced cell cycle arrest have been associated with photoaging, from flow cytometry analysis we found that there was an induction of cell cycle arrest at the G1/S phase in the UVA-bystander cells. The expression of some key photoaging marker genes likes, matrix metalloproteinases (MMP-1, MMP-3, MMP-9), cyclooxygenase-2 (COX-2), collagen1 and elastin were assessed from qRT-PCR. Upregulation of MMP-1 and COX-2, downregulation of collagen1 and elastin, along with suppression below normal expression for MMP-3 and MMP-9 was observed in the UVA-bystander A375 cells. Conclusion: Our findings for the first time suggest the contribution of UVA-bystander cells to the process of photoaging.

show abstract

Targeted and non-targeted effects of radiation in mammalian cells: An overview

Ghosh¹,

Hansda²

2021

Arch Biotechnol Biomed

View full text Add to dashboard Cite

Radiation of different wavelengths can kill living organisms, although, the mechanism of interactions differs depending on their energies. Understanding the interaction of radiation with living cells is important to assess their harmful effects and also to identify their therapeutic potential. Temporally, this interaction can be broadly divided in three stages – physical, chemical and biological. While radiation can affect all the important macromolecules of the cells, particularly important is the damage to its genetic material, the DNA. The consequences of irradiation include- DNA damage, mutation, cross-linkages with other molecules, chromosomal aberrations and DNA repair leading to altered gene expression and/or cell death. Mutations in DNA can lead to heritable changes and is important for the induction of cancer. While some of these effects are through direct interaction of radiation with the target, radiation can interact with the surrounding environment to result in its indirect actions. The effects of radiation depend not only on the total dose but also on the dose rate, LET etc. and also on the cell types. However, action of radiation on organisms is not restricted to interactions with irradiated cells, i.e. target cells alone; it also exerts non-targeted effects on neighboring unexposed cells to produce productive responses; this is known as bystander effect. The bystander effects of ionizing radiations are well documented and contribute largely to the relapse of cancer and secondary tumors after radiotherapy. Irradiation of cells with non-ionizing Ultra-Violet light also exhibits bystander responses, but such responses are very distinct from that produced by ionizing radiations.

show abstract

Role of Proteases in Photo-aging of the Skin

Cited by 3 publications

References 64 publications

The Role of Bystander Effect in Ultraviolet A Induced Photoaging

The Role of Bystander Effect in Ultraviolet A Induced Photoaging

The Role of Bystander Effect in Ultraviolet A Induced Photoaging

Targeted and non-targeted effects of radiation in mammalian cells: An overview

Contact Info

Product

Resources

About