Role of Protein Phosphatase1 Regulatory Subunit3 in Mediating the Abscisic Acid Response

Zhang, Jing; Qin, Qianqian; Nan, Xiaohui; Guo, Zilong; Liu, Yang; Jadoon, Sawaira; Chen, Yan; Zhao, Lulu; Liu, Yan; Hou, Suiwen

doi:10.1104/pp.20.01018

Cited by 15 publications

(22 citation statements)

References 55 publications

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“…The topp12546 and topp12589 lines showed similar seed germination, cotyledon expansion, and stomatal opening compared with wild-type plants (Figures S3F-S3J). Our results agree with a negative role of Arabidopsis TOPP family in ABA signaling, as previously reported (Hou et al, 2016;Zhang et al, 2020b), and suggest that the clades of TOPP1/2/5/3/7 might play a more dominant role in it. A previous study shows that TOPP1 protein inhibits SnRK2 activity, the central element transducing ABA signal (Hou et al, 2016).…”

Section: Water-soaking-inducing Avre Interacts With Arabidopsis Topp ...supporting

confidence: 92%

“…SnRK2s further activate downstream substrate proteins including ion channels (e.g., SLAC1 and SLAH3) on the plasma membrane (PM) of guard cells for stomatal closure and transcription factors for inducing ABA-responsive genes, which eventually lead to lower transpiration, minimized water loss, and other adaptive metabolic changes (Chen et al, 2020;Kuromori et al, 2018). Interestingly, in addition to PP2Cs, type one protein phosphatases (TOPPs) also negatively regulate ABA signaling, by inhibiting SnRK2 activity, in Arabidopsis (Hou et al, 2016;Zhang et al, 2020b). The topp1 and higher-order topp mutants are hypersensitive to ABA treatment (Hou et al, 2016;Zhang et al, 2020b).…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, in addition to PP2Cs, type one protein phosphatases (TOPPs) also negatively regulate ABA signaling, by inhibiting SnRK2 activity, in Arabidopsis (Hou et al, 2016;Zhang et al, 2020b). The topp1 and higher-order topp mutants are hypersensitive to ABA treatment (Hou et al, 2016;Zhang et al, 2020b). One key physiological output of ABA signaling is the closure of stomata, which serve as the entry routes for numerous bacteria to get into the tissue from the leaf surface (Melotto et al, 2008;Melotto et al, 2017;Xin et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Bacterial effectors manipulate plant abscisic acid signaling for creation of an aqueous apoplast

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Cai

et al. 2022

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Section: Water-soaking-inducing Avre Interacts With Arabidopsis Topp ...supporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial effectors manipulate plant abscisic acid signaling for creation of an aqueous apoplast

Ding

Cai

et al. 2022

Cell Host & Microbe

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“…Liquid chromatography–tandem mass spectrometry analysis was performed as previously described which mainly included alkylation, tryptic digestion, mass spectrometric and database searching (Wang et al, 2013; Zhang et al, 2020). The gels of protein bands were cut into cubic pieces and washed with 50% acetonitrile in 25 mmol/L NH 4 HCO 3 until the Coomassie brilliant blue disappeared.…”

Section: Methodsmentioning

confidence: 99%

BYPASS1‐LIKE regulates lateral root initiation via exocytic vesicular trafficking‐mediated PIN recycling in Arabidopsis

Yang

Chen

et al. 2022

JIPB

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Auxin and auxin-mediated signaling pathways are known to regulate lateral root development. Although exocytic vesicle trafficking plays an important role in recycling the PIN-FORMED (PIN) auxin efflux carriers and in polar auxin transport during lateral root formation, the mechanistic details of these processes are not well understood. Here, we demonstrate that BYPASS1-LIKE (B1L) regulates lateral root initiation via exocytic vesicular trafficking-mediated PIN recycling in Arabidopsis thaliana. b1l mutants contained significantly more lateral roots than the wild type, primarily due to increased lateral root primordium initiation. Furthermore, the auxin signal was stronger in stage I lateral root primordia of b1l than in those of the wild type. Treatment with exogenous auxin and an auxin transport inhibitor indicated that the lateral root phenotype of b1l could be attributed to higher auxin levels and that B1L regulates auxin efflux. Indeed, compared to the wild type, C-terminally green fluorescent protein-tagged PIN1 and PIN3 accumulated at higher levels in b1l lateral root primordia. B1L interacted with the exocyst, and b1l showed defective PIN exocytosis. These observations indicate that B1L interacts with the exocyst to regulate PIN-mediated polar auxin transport and lateral root initiation in Arabidopsis.

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“…LC-MS/MS analysis was performed as previously described which mainly included alkylation, tryptic digestion, mass spectrometric and database searching(Wang et al, 2013; Zhang et al, 2020). The gels of protein bands were cut into cubic pieces and washed with 50% acetonitrile in 25 mM NH 4 HCO 3 until the Coomassie brilliant blue was disappeared.…”

Section: Methodsmentioning

confidence: 99%

B1L regulates lateral root development by exocytic vesicular trafficking-mediated polar auxin transport in Arabidopsis

Yan

Bi-xia²,

Chuan³

et al. 2021

Preprint

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Auxin and auxin-mediated signaling pathways involved in the regulation of lateral root development are well documented. Although exocytic vesicle trafficking plays an important role in PIN-auxin-efflux carrier recycling, and polar auxin transport during lateral root formation, however, the mechanistic details of these processes are not well understood. Here, we demonstrate an essential regulatory mechanism of B1L that interacts with the exocyst to regulate PIN-mediated polar auxin transport and lateral root initiation. B1L is highly expressed in Arabidopsis roots, and genetic and cellular analyses have revealed that B1L is mainly involved in lateral root primordia initiation. Furthermore, DR5::GUS expression analyses revealed that auxin levels were higher in lateral root primordia of the b1l mutant than in the wild-type. Exogenous auxin treatment confirmed that the lateral root phenotype correlated closely with auxin levels. Additionally, auxin transport-inhibitory treatment indicated that B1L regulates auxin efflux. Consistently, b1l mutants exhibited higher levels of auxin efflux carriers PIN1-GFP and PIN3-GFP in lateral root primordia. Moreover, B1L interacts with the exocyst and functions in recycling PIN2-GFP. Finally, the b1l-1/exo70b1-1 double-mutant exhibited a significant increase in the number of lateral roots compared to the wildtype, b1l-1, and exo70b1-1. Collectively, this study improves our understanding of the highly sophisticated processes involved in exocytic vesicular trafficking-mediated polar auxin transport and lateral root initiation in plants.

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Role of Protein Phosphatase1 Regulatory Subunit3 in Mediating the Abscisic Acid Response

Abstract: One-sentence summary: A regulatory subunit of protein phosphatase 1 is involved in plant response to abscisic acid and abiotic stress in Arabidopsis.

Cited by 15 publications

References 55 publications

Bacterial effectors manipulate plant abscisic acid signaling for creation of an aqueous apoplast

Bacterial effectors manipulate plant abscisic acid signaling for creation of an aqueous apoplast

BYPASS1‐LIKE regulates lateral root initiation via exocytic vesicular trafficking‐mediated PIN recycling in Arabidopsis

B1L regulates lateral root development by exocytic vesicular trafficking-mediated polar auxin transport in Arabidopsis

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