Role of Pseudomonas aeruginosa lipopolysaccharides in modulation of biofilm and virulence factors of Enterobacteriaceae

Abdel-Rhman, Shaymaa H.

doi:10.1007/s13213-018-1420-5

Cited by 12 publications

(8 citation statements)

References 35 publications

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“…These binding molecules have been both successfully targeted by glycopeptide dendrimers and monosaccharides, disrupting the biofilm formation [ 50 , 51 , 52 , 53 , 54 ]. Interestingly, it has been shown that the application of LPSs from P. aeruginosa stabilizes and increases the biofilm formation of other Enterobacteriaceae [ 55 ]. In conclusion, LPSs contribute to the defense and communication system, while exopolysaccharides contribute to the biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Advances in Understanding of the Copper Homeostasis in Pseudomonas aeruginosa

Hofmann

Hirsch

Ruthstein

2021

IJMS

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Thirty-five thousand people die as a result of more than 2.8 million antibiotic-resistant infections in the United States of America per year. Pseudomonas aeruginosa (P. aeruginosa) is classified a serious threat, the second-highest threat category of the U.S. Department of Health and Human Services. Among others, the World Health Organization (WHO) encourages the discovery and development of novel antibiotic classes with new targets and mechanisms of action without cross-resistance to existing classes. To find potential new target sites in pathogenic bacteria, such as P. aeruginosa, it is inevitable to fully understand the molecular mechanism of homeostasis, metabolism, regulation, growth, and resistances thereof. P. aeruginosa maintains a sophisticated copper defense cascade comprising three stages, resembling those of public safety organizations. These stages include copper scavenging, first responder, and second responder. Similar mechanisms are found in numerous pathogens. Here we compare the copper-dependent transcription regulators cueR and copRS of Escherichia coli (E. coli) and P. aeruginosa. Further, phylogenetic analysis and structural modelling of mexPQ-opmE reveal that this efflux pump is unlikely to be involved in the copper export of P. aeruginosa. Altogether, we present current understandings of the copper homeostasis in P. aeruginosa and potential new target sites for antimicrobial agents or a combinatorial drug regimen in the fight against multidrug resistant pathogens.

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Section: Introductionmentioning

confidence: 99%

Advances in Understanding of the Copper Homeostasis in Pseudomonas aeruginosa

Hofmann

Hirsch

Ruthstein

2021

IJMS

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“…The LPS consist of lipid, core polysaccharides and o-antigen. The composition of LPS sugar affects the formation of biofilm (Abdel-Rhman, 2019), that is formed by aggregation of diverse bacteria which consist of various components like, extra polysaccharide substance (EPS), DNA, proteins etc., which assist to adhere with solid substratum. It is reported that the biofilm could enhance the PAH biodegradation and is important phase of bacteria and the biofilm formation enhance by inhibiting its flagellar gene transcription results in bacterial non- motility (Lahiri et al, 2022).…”

Section: Mechanism Of Nps-microbes Interaction In Pahs Degradationmentioning

confidence: 99%

Nano-Enhanced Microbial Remediation of PAHs Contaminated Soil

Rajput

Smita

Minkina

et al. 2023

Air, Soil and Water Research

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The emergence of polycyclic aromatic hydrocarbons (PAHs) from a variety of natural and anthropogenic sources, such as coal gasification and liquefaction plants, coke and aluminum production, catalytic cracking towers, and motor vehicle exhaust, among others, results in significant soil pollution, and a threat to human health, igniting a surge of interest in advanced research. Even though the cleanup of PAHs-contaminated areas received a great consideration. In the last decade, nanotechnology has exploded in popularity as a result of several unique properties of nanomaterials, and remediation is no exception. Thus, nano-enhanced bioremediation reported to act as a viable and effective strategy for PAHs remediation. Further, the integration of nano-enabled materials with microorganisms emerged as a promising biodegradation approach for PAHs remediation. As a result, the focus of this mini review is on depicting the possible roles of various nanomaterials in decontaminating PAHs as a green strategy by boosting the efficacy of microbial functionality, and mechanism of nanoparticles-microbes interaction in PAHs degradation. The future perspective of nano-enhanced microbial remediation of PAHs in realistic environments are also discussed.

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“…Absorbance (A 540 ) was recorded using a microtiter plate reader (BioTek ELx800). 26,33,34 Inhibition of QS Signal Molecule Supernatants of P. aeruginosa PAO1 overnight cultures treated with sub-MICs (1/4x −1/32x MIC) of tyrosol and EDTA, in addition to the supernatant of untreated cells and PAO1-JP2 (a negative control) were prepared as mentioned previously by Abdel-Rhman and Rizk. 26 Miller assay was used to measure AHLs by β-galactosidase activity determination, 35,36 utilizing E. coli DH5α/pECP61.5 and E. coli MG4/pkDT17 for measuring C4-HSL and C12-HSL, respectively.…”

Section: Effect Of Sub-mics On the Viability Of P Aeruginosamentioning

confidence: 99%

“…Quantitative Real-Time PCR P. aeruginosa was treated with tyrosol and EDTA sub-MICs, then TRI Reagent (T9424 Sigma-Aldrich) was used to extract the total RNA which was converted into cDNA using QuantiTect Reverse Transcription kit (QIAGEN, USA) as previously described. 33 The level of (lasR, lasI, rhƖI, rhIR, pqsR, pqsA, exoS, and exoY) genes expression was measured by RT-PCR utilizing the primer sequences which are listed in Table 1. rpoD gene was used as an internal P. aeruginosa housekeeping gene.…”

Section: Motility Assaysmentioning

confidence: 99%

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<p>Effect of Sub-Minimum Inhibitory Concentrations of Tyrosol and EDTA on Quorum Sensing and Virulence of <em>Pseudomonas aeruginosa</em></p>

Abdel-Rhman

Rizk

Abdelmegeed

2020

IDR

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Introduction: Pseudomonas aeruginosa is considered a dangerous pathogen, as it causes many human diseases, besides that it is resistant to almost all types of antibacterial agents. So, new strategies to overcome P. aeruginosa infection have evolved to attenuate its virulence factors and inhibit its quorum-sensing (QS) activity. Purpose: This study investigated the effect of tyrosol and EDTA as anti-quorum-sensing and antivirulence agents against P. aeruginosa PAO1. Methods: Anti-quorum activity of sub-minimum inhibitory concentrations (sub-MICs) of tyrosol and EDTA was tested using Chromobacterium violaceum (CV 12,472) biosensor bioassay. Miller assay was used to assess the inhibition of QS signal molecules by βgalactosidase activity determination. Also, their effects on the production of protease, lipase, lecithinase, and motility were tested. The inhibitory effects of these molecules on QS regulatory genes and exotoxins genes expression were evaluated by real-time PCR. Results: Tyrosol and EDTA at sub-MICs inhibited the production of violacein pigment. Both compounds inhibited QS molecules production and their associated virulence factors (protease, lipase, lecithinase, and motility) (P≤ 0.05). Besides, the expression levels of QS regulatory genes (lasI, lasR, rhƖI, rhIR, pqsA, and pqsR) and exotoxins genes (exoS and exoY) were significantly reduced (P≤ 0.05). Conclusion: Both tyrosol and EDTA can be used to fight P. aeruginosa infection as antiquorum-sensing and antivirulence agents at their sub-MICs.

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Role of Pseudomonas aeruginosa lipopolysaccharides in modulation of biofilm and virulence factors of Enterobacteriaceae

Cited by 12 publications

References 35 publications

Advances in Understanding of the Copper Homeostasis in Pseudomonas aeruginosa

Advances in Understanding of the Copper Homeostasis in Pseudomonas aeruginosa

Nano-Enhanced Microbial Remediation of PAHs Contaminated Soil

<p>Effect of Sub-Minimum Inhibitory Concentrations of Tyrosol and EDTA on Quorum Sensing and Virulence of <em>Pseudomonas aeruginosa</em></p>

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