Role of pseudorabies virus glycoprotein gI in virus release from infected cells

Abstract

The Bartha vaccine strain of pseudorabies virus has a deletion in the short unique (Us) region of its genome which includes the genes that code for glycoproteins gl and gp63 (E.

Complex between glycoproteins gI and gp63 of pseudorabies virus: its effect on virus replication

“…Virus was titrated by plaque assay in RK or PK cells. The deletion or deletion-M13 insertion mutants defective in the gene encoding gI, gp63, or both, used in this study, have been described (13,14,15). Figure 1, which summarizes previously published data, is included to facilitate the understanding of the paper.…”

“…We showed previously that both glycoproteins gIII and gI are involved in the release of the virus from rabbit kidney (RK) cells but that each of these glycoproteins affects release from these cells, mainly in conjunction with the other (3,13,23). Both glycoproteins gI and gp63 also affect virulence, an effect that is also evident only in conjunction with a defect in glycoprotein gIll (as well as possibly in conjunction with a defect in some other viral function) (14,15).…”

Studies on the immunoreactivity of native and recombinant pseudorabies virus nucleocapsid proteins

“…However, gl has not been found to be absent in any the virulent field strains of PRV tested to date (6,7,101). Glycoprotein gl also plays a role in the release of progeny virus from infected cells (7,48,66). In addition, complement mediated in vitro virus neutralization has been described using gl specific monoclonal antibody (50).…”

“…Functional studies indicate that gp63 and gl play major roles in the release of progeny virions from PRV infected cells (62,66,68,74), The host immune response to gp63 has not been described to date.…”

Estudio comparativo entre cepas del virus de la pseudorrabia aisladas en el país y de referencia mediante pruebas físico-químicas, electroforesis en SDS-PAGE y patrones de restricción del ADN

“…gE: por inmunoprecipitación ha sido demostrado que forma un complejo unido no covalentemente con gl. La ausencia de esta gp altera las características de maduración del virus influenciando en la liberación de los viriones y es específico del tipo celular (19,82,131,135). Induce la producción de gran cantidad de AcN dependientes del C' (57,82), participa en la difusión viral célula a célula y actúa además como determinante de la neurovirulencia en cerdos dependiente de la cepa (82,132,135).…”