Role of putrescine in cell proliferation in a colon carcinoma cell line

Farriol, M.; Segovia-Silvestre, Toni; Castellanos, J.M.; Venereo, Y.; Orta, Xavi

doi:10.1016/s0899-9007(01)00670-0

Cited by 34 publications

(23 citation statements)

References 22 publications

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“…( 43 ) Previous experiments with different carcinoma tissues have demonstrated a marked increase in the levels of polyamines and ornithine decarboxylase (ODC) activity, confirming their correlation with neoplastic growth and high rates of cell proliferation. ( 44,45 ) In the present study, the levels of polyamines were found to be increased in cancer‐bearing group II animals. Paclitaxel and W. somnifera combination treatment reduced the level of polyamine synthesis in cancer‐bearing animals.…”

Section: Discussionsupporting

confidence: 59%

Chemotherapeutic efficacy of paclitaxel in combination with Withania somnifera on benzo(a)pyrene‐induced experimental lung cancer

et al. 2006

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Lung cancer is one of the leading causes of cancer death in the world and is notoriously difficult to treat effectively. In the present study, male Swiss albino mice were divided into five groups of six animals each: group I animals received corn oil orally and served as a control; group II cancer-induced animals received benzo(a)pyrene (50 mg/kg bodyweight dissolved in corn oil, orally) twice weekly for four successive weeks; group III cancer-bearing animals (after 12 weeks of induction) were treated with paclitaxel (33 mg/kg bodyweight, i.p.) once weekly for 4 weeks; group IV cancer-bearing animals were treated with paclitaxel along with Withania somnifera (400 mg/kg bodyweight) orally once weekly for 4 weeks; and group V animals constituted the drug control treated with paclitaxel along with W. somnifera. The serum, lung and liver were investigated biochemically for aryl hydrocarbon hydroxylase, γ γ γ γ-glutamyl transpeptidase, 5′ ′ ′ ′-nucleotidase, lactate dehydrogenase and proteinbound carbohydrate components (hexose, hexosamine and sialic acid). These enzyme activities were increased significantly in cancer-bearing animals compared with control animals. The elevation of these in cancer-bearing animals was indicative of the persistent deteriorating effect of benzo(a)pyrene in cancerbearing animals. Our data suggest that paclitaxel, administered with W. somnifera, may extend its chemotherapeutic effect through modulating protein-bound carbohydrate levels and marker enzymes, as they are indicators of cancer. The combination of paclitaxel with W. somnifera could effectively treat the benzo(a)pyrene-induced lung cancer in mice by offering protection from reactive oxygen species damage and also by suppressing cell proliferation. (Cancer Sci 2006; 97: 658-664) L ung cancer is a major cause of morbidity and mortality worldwide both in men and women, accounting for 29% of all cancers. The incidence of lung cancer remains very high. The World Health Organization estimates that lung cancer is the most frequent cancer in the world today, and the global incidence of lung cancer is increasing at a rate of 0.5% per year due to the fact that the smoking epidemic continuous to spread to developing countries. Lung cancer is strongly related to smoking. We know that 85-95% of lung cancer patients are smokers. In many developed countries smoking prevalence is approximately the same among women and men.(1) Polycyclic aromatic hydrocarbons (PAH), many of which are carcinogenic, are widespread environmental contaminants produced by incomplete combustion. Mammalian metabolism of carcinogenic PAH mediated by cytochromes P450 and epoxide hydrolase generates, among other metabolites, bay region diol epoxides. The tumorigenic potential of these metabolites most likely results from their reaction with cellular DNA to form miscoding adducts.(2) The radical cationic forms of benzo(a)pyrene (B[a]P) may be involved in both the mechanism and metabolic activation leading to the formation of DNA adducts, which are key components of the tumo...

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Section: Discussionsupporting

confidence: 59%

Chemotherapeutic efficacy of paclitaxel in combination with Withania somnifera on benzo(a)pyrene‐induced experimental lung cancer

et al. 2006

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“…In this regard, putrescine activates T. cruzi S-adenosylmethionine decarboxylase (Clyne et al 2002). Furthermore, putrescine was shown to be required for the proliferation of carcinoma cells (Farriol et al 2001) and insect neuroblasts (Cayre et al 1997). In addition, hypoxic rat smooth muscle cells specifically require increased putrescine uptake for p38 mitogen-activated protein kinase activation (Ruchko et al 2003), and this diamine can regulate the function of several genes (Pastorian et al 2000;Fujimoto et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Putrescine analogue cytotoxicity against Trypanosoma cruzi

et al. 2005

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Trypanosoma cruzi is the etiological agent of American trypanosomiasis. Most of the available data on trypanosomatid parasites were obtained from African trypanosomes. Parasitic protozoa polyamine metabolism and transport pathways comprise valuable targets for chemotherapy. T. cruzi cannot synthesize putrescine, but its uptake from the extracellular milieu can promote parasite survival. Nevertheless, little is known about the cell biology of this diamine in T. cruzi. Here we notice that the putrescine analogue 1,4-diamino-2-butanone (DAB) inhibited T. cruzi epimastigotes' in vitro proliferation and produced remarkable mitochondrial destruction and cell architecture disorganization, as assessed by transmission electron microscopy. Mitochondrial damage was confirmed by MTT reduction. We decided to analyze the oxidative stress undergone by DAB-treated parasites. Thiobarbituric-acid-reactive substances were measured to assess lipid peroxidation. Analogue effects were dose-dependent; 5 mM DAB only slightly enhanced peroxidation, whereas 10 mM DAB significantly (P < 0.05) diminished it. These data indicate that putrescine uptake by this diamine auxotrophic parasite may be important for epimastigote axenic growth and cellular organization.

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“…Because of high proliferation rates, intestinal and colonic mucosa has a special demand for putrescine (Löser et al, 1999). The growth of murine colon tumour cells is also stimulated by newly incorporated putrescine (Farriol et al, 2001). …”

Section: Physiological Role In Humansmentioning

confidence: 99%

Scientific Opinion on risk based control of biogenic amine formation in fermented foods

Publication¹

2011

EFSA Journal

677

276

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A qualitative risk assessment of biogenic amines (BA) in fermented foods was conducted, using data from the scientific literature, as well as from European Union‐related surveys, reports and consumption data. Histamine and tyramine are considered as the most toxic and food safety relevant, and fermented foods are of particular BA concern due to associated intensive microbial activity and potential for BA formation. Based on mean content in foods and consumer exposure data, fermented food categories were ranked in respect to histamine and tyramine, but presently available information was insufficient to conduct quantitative risk assessment of BA, individually and in combination(s). Regarding BA risk mitigation options, particularly relevant are hygienic measures to minimize the occurrence of BA‐producing microorganisms in raw material, additional microbial controls and use of BA‐nonproducing starter cultures. Based on limited published information, no adverse health effects were observed after exposure to following BA levels in food (per person per meal): a) 50 mg histamine for healthy individuals, but below detectable limits for those with histamine intolerance; b) 600 mg tyramine for healthy individuals not taking monoamino oxidase inhibitor (MAOI) drugs, but 50 mg for those taking third generation MAOI drugs or 6 mg for those taking classical MAOI drugs; and c) for putrescine and cadaverine, the information was insufficient in that respect. Presently, only high‐performance liquid chromatography (HPLC)‐based methods enable simultaneous and high sensitivity quantification of all BA in foods, hence are best suited for monitoring and control purposes. Monitoring of BA concentrations in fermented foods during the production process and along the food chain would be beneficial for controls and further knowledge. Further research on BA in fermented foods is needed; particularly on toxicity and associated concentrations, production process‐based control measures, further process hygiene and/or food safety criteria development, and validation of analysis methods.

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Role of putrescine in cell proliferation in a colon carcinoma cell line

Cited by 34 publications

References 22 publications

Chemotherapeutic efficacy of paclitaxel in combination with Withania somnifera on benzo(a)pyrene‐induced experimental lung cancer

Chemotherapeutic efficacy of paclitaxel in combination with Withania somnifera on benzo(a)pyrene‐induced experimental lung cancer

Putrescine analogue cytotoxicity against Trypanosoma cruzi

Scientific Opinion on risk based control of biogenic amine formation in fermented foods

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