Role of putrescine metabolic pathways in the differentiation process of maize meristematic callus

Bernet, Esmaragda; Claparols, I.; Santos, M. Teresa; Torné, J. M.

doi:10.1016/s0981-9428(98)80027-2

Cited by 11 publications

(12 citation statements)

References 37 publications

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“…Fresh weight gain of callus pretreated with mannitol and CdCl 2 on putrescine treatment demonstrated that exogenous application of putrescine increased the growth of callus, which we speculate to be directly related to the uptake of putrescine. Bernet et al (1998) reported that about 70% of putrescine was rapidly taken up by the maize callus in putrescine-containing medium; these findings can explain our observations of fresh weight gain to a great extent. The use of the term "rapid" in this study is only in context with the time frame of 10 d of regeneration, which otherwise takes 20-25 d in culture conditions.…”

Section: Resultssupporting

confidence: 74%

Culture surface and exogenous putrescine-altered shoot growth pattern in mannitol- and cadmium chloride-pretreated callus of Cymbidium Via del Playa “Yvonne”

Guha

Rao

2010

In Vitro Cell.Dev.Biol.-Plant

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Evidence for the link between the surface of culture medium and abnormal shoot formation in the presence of exogenous putrescine is presented. Little is known about the role of putrescine in rapid shoot regeneration in the callus of orchids pretreated with mannitol and cadmium chloride (CdCl 2 ). Ultrastructural analysis of putrescine-altered CdCl 2 -and mannitol-treated callus after 4 wk of culture showed the presence of numerous oil droplets, rough endoplasmic reticulum, and dictyosomes. Callus pretreated with mannitol and CdCl 2 but without exogenous putrescine showed the presence of amyloplasts, condensed mitochondria, and active vacuole within 12 h of pretreatment. This study not only establishes a new method to achieve rapid regeneration of Cymbidium callus but also describes different types of shoots on the basis of its contact with the surface of the culture medium. Histologically, two types of cells in the apical meristem are defined. One type of cells has compromised volume which was lost upon division and is represented by small deeply stained cells of the shoots regenerated from the upper portion of the callus and is considered normal. The other type is represented by highly vacuolated and less deeply stained cells of the shoots regenerated from the lower portion of the callus in contact with the medium and is considered abnormal in this study. To avoid such abnormalities, frequent subculture of callus halves which were obtained by a median transverse cut before the regenerants (PLBs) were converted to shoots is recommended. This new methodology was successful in achieving rapid regeneration of uniform shoots from callus.

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Section: Resultssupporting

confidence: 74%

Culture surface and exogenous putrescine-altered shoot growth pattern in mannitol- and cadmium chloride-pretreated callus of Cymbidium Via del Playa “Yvonne”

Guha

Rao

2010

In Vitro Cell.Dev.Biol.-Plant

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“…The addition of polyamines increased plant regeneration in different species (Shoeb et al, 2001;Bernet et al, 1998;Chi et al, 1994). Cellular polyamine levels and their Put:Spd ratio have even been suggested as important determinants (biomarkers) of plant regeneration ability in indica rice (Shoeb et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…(Evans and Malmberg, 1989;Kakkar et al, 2000). The effect of exogenous polyamines on the shoot regeneration in different species (Chi et al, 1994;Pua et al, 1996;Bernet et al, 1998;Bais et al, 2001;Shoeb et al, 2001) and the synergistic effect of ethylene inhibitors and putrescine on shoot regeneration have also been studied (Pua et al, 1996;Bais et al, 2001). Polyamines have been regarded as a new class of plant growth regulators and hormonal second-messengers, at least in cultured tissues (see review by Kakkar et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Auxin pulses and a synergistic interaction between polyamines and ethylene inhibitors improve adventitious regeneration from apricot leaves and Agrobacterium-mediated transformation of leaf tissues

Petri

Alburquerque

Pérez‐Tornero³

et al. 2005

Plant Cell Tiss Organ Cult

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The effect, on adventitious regeneration from apricot leaf explants and transformation of leaf tissues, of auxins pulses with NAA and 2, 4-D D was tested. Addition of the polyamines putrescine and spermidine to the regeneration medium, alone or in combination with the ethylene inhibitors silver thiosulphate and aminoethoxyvinylglycine, were also tested to design a procedure that improved transformation efficiency. Spermidine at 2 mM in combination with 0.5 lM aminoethoxyvinylglycine and four-day pulses with two different concentrations of 2, 4-D D increased significantly shoot regeneration. Spermidine at the same concentration but in combination with 60 lM silver thiosulphate and four-day pulses with 9 lM 2, 4-D D also increased stable transformation events and GFP-expressing calluses probably by inducing a larger amount of dividing cells where Agrobacterium transferred its T-DNA. Since regeneration from apricot leaves occurs mostly from developing calluses, it is important to obtain many GFP-expressing calluses and, given that transformation efficiencies (number of transformed shoots per total number of explants) in woody plants are generally very low, approaches that allow the optimization of T-DNA transfer and total number of transformed cells obtained, will improve probabilities of obtaining transformed shoots.

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“…Maize (Zea mays L.) TGase was immunodetected in meristematic calli and their isolated chloroplasts, as a unique 58 kDa band. The activity was shown to be light sensitive, affected by hormone deprivation and with a light/dark rhythm (Bernet 1997;Bernet et al 1999). Subcellular localization studies showed that, in adult plants, the enzyme was specifically localized in the chloroplast grana-appressed thylakoids and close to LHCII and its abundance depended on the degree of grana development (Villalobos et al 2001;Villalobos 2007;Santos et al 2007).…”

Section: Transglutaminases and Polyaminesmentioning

confidence: 99%

“…Transglutaminases in thylakoids and photosynthetic implications Transglutaminase activity depends on Ca 2+ , GTP and light (Villalobos et al 2004;Del Duca & Fracassini 2008), which are key factors for chloroplast energetics. Transglutaminase activity was shown to be light sensitive, affected by hormone deprivation and with a light/dark rhythm (Bernet 1997;Bernet et al 1999). Subcellular localization studies showed that, the enzyme was specifically localized in the chloroplast grana-appressed thylakoids and close to LHCII (Villalobos et al 2001;Villalobos 2007;Santos et al 2007).…”

Section: Insight Into the Role Of Thylakoid Bound Polyaminesmentioning

confidence: 99%

Transglutaminase is Involved in the Remodeling of Tobacco Thylakoids

Ioannidis¹,

Torné²,

Kotzabasis³

et al. 2012

Advances in Photosynthesis - Fundamental Aspects

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Role of putrescine metabolic pathways in the differentiation process of maize meristematic callus

Cited by 11 publications

References 37 publications

Culture surface and exogenous putrescine-altered shoot growth pattern in mannitol- and cadmium chloride-pretreated callus of Cymbidium Via del Playa “Yvonne”

Culture surface and exogenous putrescine-altered shoot growth pattern in mannitol- and cadmium chloride-pretreated callus of Cymbidium Via del Playa “Yvonne”

Auxin pulses and a synergistic interaction between polyamines and ethylene inhibitors improve adventitious regeneration from apricot leaves and Agrobacterium-mediated transformation of leaf tissues

Transglutaminase is Involved in the Remodeling of Tobacco Thylakoids

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