Role of Rapamycin and 3-MA in oxidative damage of HLECs caused by two doses of UVB radiation

Yang, Hao; Ping, Xiyuan; Cui, Yaqiong; Zheng, Shaokai; Shentu, Xingchao

doi:10.1016/j.aopr.2022.09.002

Cited by 3 publications

(2 citation statements)

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“…Cataracts caused by all environmental stressors involve endoplasmic reticulum stress, which generates severe levels of ROS, thereby inducing apoptosis of LECs [ 34 , 38 , 58 ]. The antioxidant defence system composed of the tear film, cornea, and aqueous humour is essential for maintaining redox homeostasis and preventing oxidative damage in the eye [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Glycolysis Aids in Human Lens Epithelial Cells’ Adaptation to Hypoxia

Huang,

Ping,

Cui

et al. 2023

Antioxidants

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Hypoxic environments are known to trigger pathological damage in multiple cellular subtypes. Interestingly, the lens is a naturally hypoxic tissue, with glycolysis serving as its main source of energy. Hypoxia is essential for maintaining the long-term transparency of the lens in addition to avoiding nuclear cataracts. Herein, we explore the complex mechanisms by which lens epithelial cells adapt to hypoxic conditions while maintaining their normal growth and metabolic activity. Our data show that the glycolysis pathway is significantly upregulated during human lens epithelial (HLE) cells exposure to hypoxia. The inhibition of glycolysis under hypoxic conditions incited endoplasmic reticulum (ER) stress and reactive oxygen species (ROS) production in HLE cells, leading to cellular apoptosis. After ATP was replenished, the damage to the cells was not completely recovered, and ER stress, ROS production, and cell apoptosis still occurred. These results suggest that glycolysis not only performs energy metabolism in the process of HLE cells adapting to hypoxia, but also helps them continuously resist cell apoptosis caused by ER stress and ROS production. Furthermore, our proteomic atlas provides possible rescue mechanisms for cellular damage caused by hypoxia.

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Section: Discussionmentioning

confidence: 99%

Glycolysis Aids in Human Lens Epithelial Cells’ Adaptation to Hypoxia

Huang,

Ping,

Cui

et al. 2023

Antioxidants

Self Cite

View full text Add to dashboard Cite

show abstract

“…3-methyladenine (3-MA) is commonly utilized as an autophagy inhibitor and can alleviate pulmonary microvascular endothelial cell damage by decreasing apoptosis [ 13 ]. In addition, 3-MA was also shown to reduce oxidative damage and apoptosis induced by ultraviolet B radiation in cells [ 14 ]. Because 3-MA can reduce apoptosis, the utilization of 3-MA may alleviate apoptosis in lung epithelial cells, thereby alleviating ALI.…”

Section: Introductionmentioning

confidence: 99%