Role of Reactive Oxygen Species during Cell Expansion in Leaves

Schmidt, Romy; Schippers, Jos H. M.

doi:10.1104/pp.16.00426

Cited by 105 publications

(76 citation statements)

References 91 publications

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“…Increases in apoplastic H 2 O 2 in response to salinity or drought have been linked to CW stiffening and a lignification process mediated by POX activity (Passardi et al ., ), while changes in apoplastic ASC metabolism could have a regulatory effect via the removal of H 2 O 2 (De Pinto and De Gara, ; Ros‐Barceló et al ., ). In addition, POX activity could promote cell elongation via loosening of the by the oxidative cleavage of polymers such as xyloglucan and pectin (Tenhaken, ; Schmidt et al ., ). Wheat cultivars tolerant to drought were shown to increase the content of pectins rhamnogalacturonan I and II in response to drought, potentially by promotion of the formation of hydrated gels which ameliorate cell damage (Leucci et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Indeed the activities of either or both of these could be altered by direct oxidation and formation of secondary radicals (Ye and Steudle, ) or indirectly through lipid membrane oxidation, which would consequently reduce g m . According to this, it has been said that abiotic stress conditions which result in increased H 2 O 2 levels promote low abundance of aquaporins at the plasma membrane in Arabidopsis thaliana , and that this can be prevented by high CAT abundance (Boursiac et al ., ; Schmidt et al ., ).…”

Section: Discussionmentioning

confidence: 97%

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The apoplastic antioxidant system and altered cell wall dynamics influence mesophyll conductance and the rate of photosynthesis

et al. 2019

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Summary Mesophyll conductance (gm), the diffusion of CO2 from substomatal cavities to the carboxylation sites in the chloroplasts, is a highly complex trait driving photosynthesis (net CO2 assimilation, AN). However, little is known concerning the mechanisms by which it is dynamically regulated. The apoplast is considered as a ‘key information bridge’ between the environment and cells. Interestingly, most of the environmental constraints affecting gm also cause apoplastic responses, cell wall (CW) alterations and metabolic rearrangements. Since CW thickness is a key determinant of gm, we hypothesize that other changes in this cellular compartiment should also influence gm. We study the relationship between the antioxidant apoplastic system and CW metabolism and the gm responses in tobacco plants (Nicotiana sylvestris L.) under two abiotic stresses (drought and salinity), combining in vivo gas‐exchange measurements with analyses of antioxidant activities, CW composition and primary metabolism. Stress treatments imposed substantial reductions in AN (58–54%) and gm (59%), accompanied by a strong antioxidant enzymatic response at the apoplastic and symplastic levels. Interestingly, apoplastic but not symplastic peroxidases were positively related to gm. Leaf anatomy remained mostly stable; however, the stress treatments significantly affected the CW composition, specifically pectins, which showed significant relationships with AN and gm. The treatments additionally promoted a differential primary metabolic response, and specific CW‐related metabolites including galactose, glucosamine and hydroxycinnamate showed exclusive relationships with gm independent of the stress. These results suggest that gm responses can be attributed to specific changes in the apoplastic antioxidant system and CW metabolism, opening up more possibilities for improving photosynthesis using breeding/biotechnological strategies.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

The apoplastic antioxidant system and altered cell wall dynamics influence mesophyll conductance and the rate of photosynthesis

et al. 2019

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“…2). In addition to their role in stress 263 responses, extracellular ROS have also been implicated in leaf cell expansion (Schmidt et al, 2016), and the 264 rbohd rbohf double mutant displays reduced rosette size (Torres et al, 2002). Overexpression of CRKs has 265 been associated with increased SA accumulation (Acharya et al, 2007;Chen et al, 2004).…”

Section: Discussion 257mentioning

confidence: 99%

CRK2 and C-terminal phosphorylation of NADPH oxidase RBOHD regulate ROS production in Arabidopsis

Kimura

Hunter

Vaahtera

et al. 2019

Preprint

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40Reactive oxygen species (ROS) are important messengers in eukaryotic organisms and their production is 41 tightly controlled. Active extracellular ROS production by NADPH oxidases in plants is triggered by 42 receptor-like protein kinase (RLK)-dependent signaling networks. Here we show that the cysteine-rich RLK 43 CRK2 kinase activity is required for plant growth and CRK2 exists in a preformed complex with the 44 NADPH oxidase RBOHD in Arabidopsis. Functional CRK2 is required for the full elicitor-induced ROS 45 burst and consequently the crk2 mutant is impaired in defense against the bacterial pathogen Pseudomonas 46 syringae pv. tomato DC3000. Our work demonstrates that CRK2 regulates plant innate immunity. We 47 identified in vitro CRK2-dependent phosphorylation sites in the C-terminal region of RBOHD. 48Phosphorylation of S703 RBOHD is enhanced upon flg22 treatment and substitution of S703 with alanine 49 reduced ROS production in Arabidopsis. Phylogenetic analysis suggests that phospho-sites in C-terminal 50 region of RBOHD are conserved throughout the plant lineage and between animals and plants. We propose 51

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“…Mitochondria produce structures that are partly homologous to the chloroplast stromules, in 349 (Schmidt et al, 2016) response to light and other stimuli in an endoplasmic reticulum (ER)-350 mediated manner. The protrusion-driven movement and positioning is considered to promote 351 the inter-compartmental trafficking of metabolites and proteins but there remains a paucity of 352 data on which proteins are trafficked and the mechanisms involved.…”

Section: Redox-dependent Formation Of Of Stromules Matrixules and Pementioning

confidence: 99%

“…The protrusion-driven movement and positioning is considered to promote 351 the inter-compartmental trafficking of metabolites and proteins but there remains a paucity of 352 data on which proteins are trafficked and the mechanisms involved. ROS and redox cues 353 modify microtubule orientation and behaviour within cells, as well as the operation of protein 354 import and export machineries (Schmidt et al, 2016). So far, it remains to be determined if 355 these organelle-derived tubular structures are involved in direct exchange of metabolites or 356 macromolecules between compartments, or might rather have a supportive function in the 357 communication between organelles by acting as an cellular anchor to temporary fix their 358 relative position to each other.…”

Section: Redox-dependent Formation Of Of Stromules Matrixules and Pementioning

confidence: 99%

On the move: Redox –dependent protein relocation

Foyer

Baker

Wright

et al. 2019

Preprint

Self Cite

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Compartmentation of proteins and processes is a defining feature of eukaryotic cells. The growth and development of organisms is critically dependent on the accurate sorting of proteins within cells. The mechanisms by which cytosol-synthesized proteins are delivered to the membranes and membrane compartments have been extensively characterised. However, the protein complement of any given compartment is not precisely fixed and some proteins can move between compartments in response to metabolic or environmental triggers. The mechanisms and processes that mediate such relocation events are largely uncharacterized. Many proteins can in addition perform multiple functions, catalyzing alternative reactions or performing structural, non-enzymatic functions. These alternative functions can be equally important functions in each cellular compartment. Such proteins are generally not dual targeted proteins in the classic sense of having targeting sequences that directde novosynthesised proteins to specific cellular locations. Accumulating evidence suggests that redox post-translational modifications (PTMs) can control the compartmentation of many such proteins, including antioxidant and/or redox associated enzymes.

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Role of Reactive Oxygen Species during Cell Expansion in Leaves

Cited by 105 publications

References 91 publications

The apoplastic antioxidant system and altered cell wall dynamics influence mesophyll conductance and the rate of photosynthesis

The apoplastic antioxidant system and altered cell wall dynamics influence mesophyll conductance and the rate of photosynthesis

CRK2 and C-terminal phosphorylation of NADPH oxidase RBOHD regulate ROS production in Arabidopsis

On the move: Redox –dependent protein relocation

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