2014
DOI: 10.1021/jp503808z
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Role of Red-Ox Cycle in Structural Oscillations and Solvation Dynamics in the Mitochondria of a Live Cell

Abstract: Structural oscillations and solvation dynamics in the mitochondria of a live cell are studied by time-resolved microscopy using a covalent fluorescence probe. We compared the dynamics in a human breast cancer cell (MCF-7) with that in a normal breast cell MCF-10A. The probe, CPM (7-diethylamino-3-(4-maleimido-phenyl)-4-methylcoumarin), binds with the free thiol groups. In MCF-10A cell, CPM binds with the discrete mitochondria. In MCF-7, CPM labels the clustered mitochondria in the peri-nuclear region. Location… Show more

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Cited by 15 publications
(36 citation statements)
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“…Representative graphs of such frequent oscillation were shown from three randomly selected time windows of 3 sec (Figure B, ii‐iv). The time period of fluorescence oscillation was calculated by determining the peak position by smoothening the intensity vs. time plot using a fast Fourier transform filter . We find that the period of oscillation is not constant and the half‐period varies from 0.35 to 0.55 sec, with an average of 0.45±0.1 sec.…”
Section: Resultsmentioning
confidence: 94%
“…Representative graphs of such frequent oscillation were shown from three randomly selected time windows of 3 sec (Figure B, ii‐iv). The time period of fluorescence oscillation was calculated by determining the peak position by smoothening the intensity vs. time plot using a fast Fourier transform filter . We find that the period of oscillation is not constant and the half‐period varies from 0.35 to 0.55 sec, with an average of 0.45±0.1 sec.…”
Section: Resultsmentioning
confidence: 94%
“…From Equation (1), the λemmax at 510–515 nm corresponds to n ≈13 Au atoms in the NC (assuming E f ≈5.53 eV, as in bulk Au) . The slight (≈5 nm) blueshift of λemmax of Au‐NCs in MCF7 cells relative to that in MCF10A cells might be due to slight variation in the microenvironment between the two cells …”
Section: Resultsmentioning
confidence: 99%
“…[5,6,[13][14][15] The slight( % 5nm) blueshift of l max em of Au-NCsi nM CF7 cells relative to that in MCF10A cells might be due to slight variation in the microenvironment between the two cells. [36] The somewhat drastic change ( % 35 nm redshift in l max em and change in size from Au 8 in bulk water to Au 13 inside the cell) in Au-NCsm ight be due to the interaction of cyt c-capped Au-NCs with different biomoleculesi nside the cell. One such candidate is GSH.…”
Section: Human Breast Cancerc Ells (Mcf7) and Non-cancer Breast Epithmentioning
confidence: 99%
“…The experimental set-up for confocal microscopy has been described in our previous publications. 40,41 To summarize, a combination of confocal microscope (Olympus IX-71) and TCSPC setup (PicoQuant, Micro-Time 200, numerical aperture (NA)≈1.2) and a pulsed picosecond diode laser (PDL 828-S "SEPIA II," Pico Quant, at 470 nm) were used. The diffraction limited spot size is 0.6λ/1.2∼ λ/2.…”
Section: 2c Experimental Set-up For Confocal Microscopymentioning
confidence: 99%
“…The emission spectra of TPPS supramolecular structures were recorded using an electron multiplying charge-coupled device (EMCCD, ANDOR Technology) attached to a spectrograph (ANDOR Technology, Shamrock series). 40,41 2.2c3 Fluorescence lifetime measurement: For recording instrument response function (IRF), we used a bare slide and collected the scattered laser light. The FWHM of the IRF for excitation at 470 nm is ∼70 ps.…”
Section: 2c2 Emission Spectramentioning
confidence: 99%