Role of Regulatory Proteins Involved in Iron Homeostasis in Pulmonary Tuberculosis Patients and Their Household Contacts

Panda, Sudhasini; Faisal, Shah; Kumar, Kiran; Seelan, Diravya M; Sharma, Alpana; Gupta, Neeraj; Datta, Sudip Kumar; Singh, Archana

doi:10.1007/s12291-020-00947-w

Cited by 5 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, a previous study also determined that transferrin levels were significantly lower in PTB and household contacts compared with HCs . Sun et al also confirmed plasma transferrin levels were significantly decreased in the PTB group samples than that in the and HC group, and the AUC value was 0.723.…”

Section: Discussionmentioning

confidence: 85%

“…Moreover, a previous study also determined that transferrin levels were significantly lower in PTB and household contacts compared with HCs. 37 Sun et al 23 also confirmed plasma transferrin levels were significantly decreased in the PTB group samples than that in the and HC group, and the AUC value was 0.723. Consistent with these studies, our studies found serum transferrin levels were significantly lower in TB than healthy controls, pneumonia and lung cancer patients which indicated TRF may be promising markers to explore the pathogenesis of TB.…”

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

Validation of Serotransferrin in the Serum as Candidate Biomarkers for the Diagnosis of Pulmonary Tuberculosis by Label-Free LC/MS

Gao

et al. 2022

ACS Omega

View full text Add to dashboard Cite

This study aimed to identify secreted protein biomarkers in serum from the label-free LC/MS proteomics of neutrophils in pulmonary tuberculosis (TB) patients for the diagnosis biomarkers of TB label-free LC/MS. The proteomic profiles of neutrophils from 15 active TB patients and 15 healthy controls (HCs) were analyzed using label-free LC/MS. We identified 358 differentially expressed proteins preliminarily, including 279 up-regulated proteins and 79 down-regulated proteins. Thirty-eight differentially expressed secreted proteins involved in the progress of platelet degranulation between TB patients and HCs were focused. Of these, serotransferrin (TRF), alpha-2-macroglobulin (AMG), alpha-1-antitrypsin (AAT), alpha-1-acid glycoprotein 1 (AAG), alpha-1-acid glycoprotein 2 (AGP2), and alpha-1B-glycoprotein (A1BG) were selected for further verification in the serum of additional 134 TB patients and 138 HCs by nephelometry and ELISA in the training set. Statistically significant differences of TRF ( P < 0.0001), AAT ( P < 0.0001), AAG ( P < 0.0001), AGP2 ( P < 0.0001), and A1BG ( P = 0.0003) were observed. The serum concentration of TRF was down-regulated in TB patients compared with healthy controls, which was coincident with the proteomics results. An additional validation of TRF was performed in an independent cohort of patients with active TB ( n = 46), patients with lung cancer ( n = 37), 20 HCs, and patients with pneumonia ( n = 35) in the test set by nephelometry. The serum expression levels of TRF in the TB patients showed lower levels compared with those in patients with pneumonia ( P = 0.0125), lung cancer ( P = 0.0005), HCs ( P < 0.0001), and the non-TB controls ( P < 0.0001). Furthermore, the AUC value of TRF was 0.647 with 90.22% sensitivity and 42.86% specificity in discriminating the TB group from the pneumonia group, 0.702 with 93.48% sensitivity and 47.16% specificity in discriminating the TB group from the lung cancer group, 0.894 with 91.30% sensitivity and 71.62% specificity in discriminating the TB group from all HCs, and 0.792 with 91.30% sensitivity and 58.90% specificity in discriminating the TB group from the non-TB controls. This study obtained the proteomic profiles of neutrophils in the TB patients and HCs, which contribute to a better understanding of the pathogenesis molecules existing in the neutrophils of pulmonary tuberculosis and provide candidate biomarkers for the diagnosis of pulmonary tuberculosis.

show abstract

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 87%

Validation of Serotransferrin in the Serum as Candidate Biomarkers for the Diagnosis of Pulmonary Tuberculosis by Label-Free LC/MS

Gao

et al. 2022

ACS Omega

View full text Add to dashboard Cite

show abstract

“…Increased extracellular and iron stores or inflammatory stimulation can trigger hepcidin expression. Hepcidin expression can be inhibited through hypoxia and erythropoiesis [17]. Hepcidin-modulated erythropoiesis to improve anemia.…”

Section: Resultsmentioning

confidence: 99%

The efficacy of Bloso fish (Glossogobius giuris sp.) in improving hemoglobin, hematocrit, platelet, and albumin levels of Wistar rats with hypoalbuminemia

Anwar

Budiono

Putriningtyas

et al. 2023

Potr. S. J. F. Sci.

View full text Add to dashboard Cite

Tuberculosis (TB) is an infectious disease worldwide that causes death. Common clinical manifestations of patients with TB include anemia, hypoalbuminemia, and malnutrition. Most patients with TB are infected with coccus bacteria, such as Staphylococcus aureus, that commonly attack the respiratory tract. However, the consumption of heme protein sources could improve the nutritional status of patients with TB. Fish comprise one of the most widely consumed sources of heme. The bloso fish (Glossogobius giuris sp.), considered a fish without economic value is a new alternative source of heme protein. This study aimed to develop supplements using bloso fish (Glossogobius giuris sp.). This study used an experimental pretest-post-test control group design. Seven male Wistar rats were used as the negative control group. Twenty- eight male Wistar rats were administered S. aureus, fed a protein-deficient diet, and divided into the positive control group, the K1 group, which received up to 675 mg/200 g of bloso fish flour, the K2 group, which received up to 67.5 mg/200 g of bloso fish oil, and the K3 group, which received up to 675 mg/200 g of bloso fish fluor from oil extraction dregs. Treatment was administered for 28 days. The hemoglobin (Hb), hematocrit (Ht), platelet, and albumin levels in blood serum from the retroorbital vein were measured. Data were processed using a paired t-test and one-way analysis of variance. The results showed differences in Hb, Ht, platelet, and albumin levels were observed before and after treatment. Additionally, differences in Hb, Ht, platelet, and albumin levels were observed in the groups that received bloso fish flour and bloso fish oil. Bloso fish flour and bloso fish oil increased the Hb, Ht, platelet, and albumin levels of rats with hypoalbuminemia.

show abstract

“…Given that host iron status impacts TB disease severity, indicators of host iron metabolism may be useful for predicting disease progression. Panda et al observed plasma iron and hemoglobin levels were low in PTB patients compared to HHC and HCs, suggesting anemia of inflammation due to active disease (21). In an earlier study, Minchella et al (70) observed higher ferritin and hepcidin concentrations among early TB-progressors compared to delayed progressors (mean ferritin 50.2 vs. 26.2 ng/ml; p = 0.027; mean hepcidin 37.7 vs. 5.6 ng/ml; p = 0.036), while low transferrin around the time of Mtb exposure was identified as a risk factor for all progressors (70).…”

Section: The Diagnostic Potential Of Host Iron Metabolism Indicatorsmentioning

confidence: 99%

“…Ferroportin (FPN-1) exports iron from macrophages into the bloodstream and is therefore central to maintaining iron homeostasis. During infection, inflammatory cytokines, primarily IL-6, induce expression of the hormone hepcidin to trigger FPN-1 internalization, preventing iron export (21); a response which can lead to anemia in chronic infections like TB (22). While macrophages represent a significant iron reservoir in the host (23,24), inflammatory cytokines also induce ferritin synthesis in macrophages thereby reducing the labile iron pool.…”

Section: Iron Homeostasis and Mtb Pathogenesismentioning

confidence: 99%

Host and Bacterial Iron Homeostasis, an Underexplored Area in Tuberculosis Biomarker Research

2021

View full text Add to dashboard Cite

Mycobacterium tuberculosis (Mtb) “a human adapted pathogen” has found multiple ways to manipulate the host immune response during infection. The human immune response to Mtb infection is a highly complex cascade of reactions, with macrophages as preferred intracellular location. Interaction with the host through infection gives rise to expression of specific gene products for survival and multiplication within the host. The signals that the pathogens encounter during infection cause them to selectively express genes in response to signals. One strategy to identify Mtb antigens with diagnostic potential is to identify genes that are specifically induced during infection or in specific disease stages. The shortcomings of current immunodiagnostics include the failure to detect progression from latent infection to active tuberculosis disease, and the inability to monitor treatment efficacy. This highlights the need for new tuberculosis biomarkers. These biomarkers should be highly sensitive and specific diagnosing TB infection, specifically distinguishing between latent infection and active disease. The regulation of iron levels by the host plays a crucial role in the susceptibility and outcome of Mtb infection. Of interest are the siderophore biosynthetic genes, encoded by the mbt-1 and mbt-2 loci and the SUF (mobilization of sulphur) operon (sufR-sufB-sufD-sufC-csd-nifU-sufT), which encodes the primary iron-sulphur cluster biogenesis system. These genes are induced during iron limitation and intracellular growth of Mtb, pointing to their importance during infection.

show abstract

Role of Regulatory Proteins Involved in Iron Homeostasis in Pulmonary Tuberculosis Patients and Their Household Contacts

Cited by 5 publications

References 21 publications

Validation of Serotransferrin in the Serum as Candidate Biomarkers for the Diagnosis of Pulmonary Tuberculosis by Label-Free LC/MS

Validation of Serotransferrin in the Serum as Candidate Biomarkers for the Diagnosis of Pulmonary Tuberculosis by Label-Free LC/MS

The efficacy of Bloso fish (Glossogobius giuris sp.) in improving hemoglobin, hematocrit, platelet, and albumin levels of Wistar rats with hypoalbuminemia

Host and Bacterial Iron Homeostasis, an Underexplored Area in Tuberculosis Biomarker Research

Contact Info

Product

Resources

About