Role of reproductive fluids and extracellular vesicles in embryo–maternal interaction during early pregnancy in cattle

Cajas, Y. N.; Cañón-Beltrán, K.; Blanca, María Gemma Millán de la; Sánchez, José María; Fernández-Fuertes, Beatriz; González, Esther Sánchez-Pardo; Rizos, Dimitrios

doi:10.1071/rd21275

Cited by 8 publications

(4 citation statements)

References 175 publications

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“…The unique tubal liquid microenvironment contains a variety of molecules, including tubal extracellular vesicles (both exosomes and microvesicles), specific glycoproteins as oviductin, nutrients such as glucose, lactate, pyruvate and amino acids, and bicarbonate ions. All these components provide an optimal environment for sperm survival and capacitation, fertilization and early embryo development in the uterine tube ( Harris et al, 2020 ; Cajas et al, 2021 ; Zhao et al, 2022 ). The volume, chemical composition and even temperature of the tubal fluid is influenced by the cyclically changing level of female sex hormones ( Saint-Dizier et al, 2019 ).…”

Section: The Role Of Tubal Fluid In Immune Privilege Of Uterine Tubementioning

confidence: 99%

Morphology of the immune cells in the wall of the human uterine tube and their possible impact on reproduction—uterine tube as a possible immune privileged organ

Visnyaiová,

Varga,

Feitscherová

et al. 2024

Front. Cell Dev. Biol.

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The uterine tube, as well as other parts of the upper female reproductive system, is immunologically unique in its requirements for tolerance to allogenic sperm and semi-allogenic embryos, yet responds to an array of sexually transmitted pathogens. To understand this dichotomy, there is a need to understand the functional morphology of immune cells in the wall of the uterine tube. Thus, we reviewed scientific literature regarding immune cells and the human uterine tube by using the scientific databases. The human uterine tube has a diverse population of immunocompetent cells representing both the innate and adaptive immune systems. We describe in detail the possible roles of cells of the mononuclear phagocyte system (macrophages and dendritic cells), T and B lymphocytes, natural killer cells, neutrophils and mast cells in association with the reproductive functions of uterine tubes. We are also discussing about the possible “immune privilege” of the uterine tube, as another mechanism to tolerate sperm and embryo without eliciting an inflammatory immune response. In uterine tube is not present an anatomical blood-tissue barrier between antigens and circulation. However, the immune cells of the uterine tube probably represent a type of “immunological barrier,” which probably includes the uterine tube among the immunologically privileged organs. Understanding how immune cells in the female reproductive tract play roles in reproduction is essential to understand not only the mechanisms of gamete transport and fertilization as well as embryo transport through the uterine tube, but also in improving results from assisted reproduction.

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Section: The Role Of Tubal Fluid In Immune Privilege Of Uterine Tubementioning

confidence: 99%

Morphology of the immune cells in the wall of the human uterine tube and their possible impact on reproduction—uterine tube as a possible immune privileged organ

Visnyaiová,

Varga,

Feitscherová

et al. 2024

Front. Cell Dev. Biol.

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“…Un modo de lograr este objetivo sería mediante la adición de fluidos reproductivos de fases específicas del ciclo estral en los medios de cultivo. Los fluidos folicular, oviductal y uterino contienen diversos elementos reguladores específicos, como hormonas, factores de crecimiento y citoquinas (Block et al, 2011;Cajas et al, 2021), carbohidratos, iones, lípidos, fosfolípidos y proteínas (Avilés et al, 2010), que, al ser agregados a los medios de cultivo, son capaces de mejorar el desarrollo de los embriones preimplantatorios obtenidos por FIV (Hamdi et al, 2018).…”

Section: Introductionunclassified

Efecto De Las Secreciones De Organoides De Endometrio Humano Sobre El Cultivo in Vitro De Embriones Bovinos

Masegosa Domínguez,

Romero-Aguirregomezcorta,

Juan

et al. 2024

An. Vet. Murcia

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Over the last 50 years, the fertility of high-producing dairy cows has declined due to the intensity of genetic selection. To overcome this obstacle, assisted reproductive techniques have been used. However, in vitro embryo production is a relatively inefficient process, with maximum blastocyst yield rates of around 35%. To overcome this problem, it was hypothesised that by adding endometrial organoid secretions to the culture medium, embryos produced in vitro would be exposed to a more physiological environment and the yield of the technique would increase. For this purpose, bovine oocytes were collected from a local abattoir and matured in vitro, fertilised in vitro and cultured in vitro. The embryo culture medium was supplemented at 1% (v/v) with i) human endometrial organoid secretions (as bovine secretions are not available), ii) Boretto culture medium, and ii) matrigel drops with Boretto culture medium, as procedural controls. In addition, a control group without any supplementation was included. Embryo development was assessed at days 2, 7 and 8 of culture. The percentage of blastocysts at day 7 and day 8 post insemination was significantly higher (P<0.05) when Boretto culture medium was added. In addition, this treatment increased embryo development kinetics at day 7 post insemination, and blastocyst quality as assessed by total cell number, with 85.8% more than the control. Although human endometrial secretions have not given the expected result, it would be necessary to test whether bovine endometrial-specific organoid secretions, at different concentrations, could increase the yield of bovine embryo production in vitro. Durante los últimos 50 años, la fertilidad de las vacas lecheras de alta producción ha disminuido debido a la intensidad de la selección genética. Para superar este obstáculo, se ha recurrido a las técnicas de reproducción asistida. Sin embargo, la producción de embriones in vitro es un proceso relativamente ineficiente, con tasas máximas de blastocistos obtenidos de alrededor del 35%. Para solventar este problema, se planteó la hipótesis de añadir al medio de cultivo secreciones de organoides de endometrio, así, los embriones producidos in vitro estarían expuestos a un ambiente más fisiológico y el rendimiento de la técnica aumentaría. Para ello, se llevó a cabo la recolección de ovocitos bovinos procedentes de un matadero local, y se procedió a su maduración in vitro, fecundación in vitro y cultivo embrionario in vitro. El medio de cultivo embrionario se suplementó al 1% (v/v) con i) secreciones de organoides de endometrio humano (ya que no existe disponibilidad de secreciones bovinas), ii) medio de cultivo Boretto, y ii) gotas de matrigel con medio de cultivo Boretto, como controles de procedimiento. Además, se incluyó un grupo control sin ningún suplemento. Se evaluó el desarrollo embrionario a los días 2, 7 y 8 de cultivo. El porcentaje de blastocistos a día 7 y a día 8 post inseminación fue significativamente mayor (P<0.05) cuando se añadió el medio de cultivo Boretto. Además, este tratamiento incrementó la cinética de desarrollo de los embriones a día 7 post inseminación, y la calidad de los blastocistos evaluada por el número total de células, con un 85.8% más que el control. Aunque las secreciones de endometrio humano no han dado el resultado esperado, sería necesario comprobar si las secreciones de organoides específicas de endometrio bovino, a distintas concentraciones, podrían incrementar el rendimiento de la producción in vitro de embriones bovinos.

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“…Different approaches have been used to understand the mechanisms of the oviduct's contribution to early reproductive events, such as early embryo development [1]. Initially, it was believed that indirect intercellular communication occurred through the transfer of secreted molecules called embryotropins [2].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway

Cañón-Beltrán,

Cajas,

Almpanis

et al. 2024

Biol Res

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Background Extracellular vesicles (EVs) and their cargoes, including MicroRNAs (miRNAs) play a crucial role in cell-to-cell communication. We previously demonstrated the upregulation of bta-mir-148b in EVs from oviductal fluid of cyclic cows. This miRNA is linked to the TGF-β pathway in the cell proliferation. Our aim was to verify whether miR-148b is taken up by embryos through gymnosis, validate its target genes, and investigate the effect of miR-148b supplementation on early embryo development and quality. Methods Zygotes were cultured in SOF + 0.3% BSA (Control) or supplemented with: 1 µM miR-148b mimics during: D1-D7 (miR148b) or D1-D4 (miR148b-OV: representing miRNA effect in the oviduct) or D4-D7 (miR148b-UT: representing miRNA effect in the uterus) or 1 µM control mimics was used during: D1-D7 (CMimic). Embryos at ≥ 16-cells and D7 blastocysts (BD7) were collected to examine the mRNA abundance of transcripts linked to the TGF-β pathway (TGFBR2, SMAD1, SMAD2, SMAD3, SMAD5, BMPR2, RPS6KB1, POU5F1, NANOG), total cell number (TC), trophectoderm (TE), and inner cell mass (ICM) were also evaluated. One-way ANOVA was used for all analyses. Results We demonstrated that miR-148b can be taken up in both 16-cell embryos and BD7 by gymnosis, and we observed a decrease in SMAD5 mRNA, suggesting it’s a potential target of miR-148b. Cleavage and blastocysts rates were not affected in any groups; however, supplementation of miR-148b mimics had a positive effect on TC, TE and ICM, with values of 136.4 ± 1.6, 92.5 ± 0.9, 43.9 ± 1.3 for miR148b and 135.3 ± 1.5, 92.6 ± 1.2, 42.7 ± 0.8, for miR148b-OV group. Furthermore, mRNA transcripts of SMAD1 and SMAD5 were decreased (P ≤ 0.001) in 16-cell embryos and BD7 from miR148b and miR148b-OV groups, while POU5F1 and NANOG were upregulated (P ≤ 0.001) in BD7 and TGFBR2 was only downregulated in 16-cell embryos. pSMAD1/5 levels were higher in the miR148b and miR148b-OV groups. Conclusions Our findings suggest that supplementation of bta-miR-148b mimics during the entire culture period (D1 - D7) or from D1 - D4 improves embryo quality and influences the TGF-β signaling pathway by altering the transcription of genes associated with cellular differentiation and proliferation. This highlights the importance of miR-148b on embryo quality and development.

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Role of reproductive fluids and extracellular vesicles in embryo–maternal interaction during early pregnancy in cattle

Cited by 8 publications

References 175 publications

Morphology of the immune cells in the wall of the human uterine tube and their possible impact on reproduction—uterine tube as a possible immune privileged organ

Morphology of the immune cells in the wall of the human uterine tube and their possible impact on reproduction—uterine tube as a possible immune privileged organ

Efecto De Las Secreciones De Organoides De Endometrio Humano Sobre El Cultivo in Vitro De Embriones Bovinos

MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway

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