This study aims to investigate the influence of welding process on the elastic lattice deformation and its effects on fatigue and fracture behavior of S355 G10+M steel, which is widely used in fabrication of offshore wind turbine monopile structures. In situ neutron diffraction measurements were taken on cross-weld test samples at room temperature to monitor the evolution of intergranular strains under static and cyclic loading conditions. Both static and cyclic test results have shown that the {200} orientation exhibits the least load carrying capacity while {211} had the maximum stiffness. The hkl-specific response predicted using Reuss and Kröner model were found to agree well with experimental values obtained for the heat-affected zone for all the orientations; however, discrepancies between the experimental and model predictions have been observed for the base metal and weld metal. Moreover, the microstructural differences between the weld metal and heat-affected zone resulted in the maximum elastic–plastic strain mismatch at the interface of the two regions. The results from this experiment would be useful to understand the role of crystal-specific microstrains and lattice deformation on fatigue and fracture behavior of thick-walled monopile weldments.