Role of Riboflavin in Induced Resistance against Fusarium Wilt and Charcoal Rot Diseases of Chickpea

Saikia, Ratul; Yadav, Mukesh; Varghese, Saju; Singh, Bhim Pratap; Gogoi, Dhrubajyoti; Kumar, Rohtash; Arora, Dilip K.

doi:10.5423/ppj.2006.22.4.339

Cited by 35 publications

(21 citation statements)

References 28 publications

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“…Plants inoculated with BRp3 exhibited an increase in the activity of POD, CAT, and PAL after 24 and 48 h post inoculation while, PPO activity was observed even after 72 h and up-to 6 days after challenge inoculation. The production of plant defensive enzymes i.e., POD, PPO, CAT, and PAL in response to inoculated PGPR bacteria is associated with ISR in plants against the pathogen (Saikia et al, 2006; Liang et al, 2011). …”

Section: Discussionmentioning

confidence: 99%

Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

et al. 2017

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Xanthomonas oryzae pv. oryzae (Xoo) is widely prevalent and causes Bacterial Leaf Blight (BLB) in Basmati rice grown in different areas of Pakistan. There is a need to use environmentally safe approaches to overcome the loss of grain yield in rice due to this disease. The present study aimed to develop inocula, based on native antagonistic bacteria for biocontrol of BLB and to increase the yield of Super Basmati rice variety. Out of 512 bacteria isolated from the rice rhizosphere and screened for plant growth promoting determinants, the isolate BRp3 was found to be the best as it solubilized 97 μg/ mL phosphorus, produced 30 μg/mL phytohormone indole acetic acid and 15 mg/ L siderophores in vitro. The isolate BRp3 was found to be a Pseudomonas aeruginosa based on 16S rRNA gene sequencing (accession no. HQ840693). This bacterium showed antagonism in vitro against different phytopathogens including Xoo and Fusarium spp. Strain BRp3 showed consistent pathogen suppression of different strains of BLB pathogen in rice. Mass spectrometric analysis detected the production of siderophores (1-hydroxy-phenazine, pyocyanin, and pyochellin), rhamnolipids and a series of already characterized 4-hydroxy-2-alkylquinolines (HAQs) as well as novel 2,3,4-trihydroxy-2-alkylquinolines and 1,2,3,4-tetrahydroxy-2-alkylquinolines in crude extract of BRp3. These secondary metabolites might be responsible for the profound antibacterial activity of BRp3 against Xoo pathogen. Another contributing factor toward the suppression of the pathogen was the induction of defense related enzymes in the rice plant by the inoculated strain BRp3. When used as an inoculant in a field trial, this strain enhanced the grain and straw yields by 51 and 55%, respectively, over non-inoculated control. Confocal Laser Scanning Microscopy (CLSM) used in combination with immunofluorescence marker confirmed P. aeruginosa BRp3 in the rice rhizosphere under sterilized as well as field conditions. The results provide evidence that novel secondary metabolites produced by BRp3 may contribute to its activity as a biological control agent against Xoo and its potential to promote the growth and yield of Super Basmati rice.

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Section: Discussionmentioning

confidence: 99%

Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

et al. 2017

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“…Ryals et al (1996) and Delaney (1997) presented evidence that riboflavin acts as a novel signaling pathway leading to systemic resistance, activating PR-genes in A. thaliana and tobacco and induces resistance to pathogens. Our preliminary studies showed that riboflavin causes induced resistance in chickpea against Fusarium wilt and charcoal rot diseases (Saikia et al, 2006).…”

Section: Introductionmentioning

confidence: 89%

“…Ten seeds were sown in a pot and maintained in a greenhouse. After 15 days of sowing, pots were inoculated with 100 ml homogenate culture (10 3 CFU/ml) prepared before, with inculcation method described by Saikia et al (2006). Four pots were used for each isolate as a replicates; each pot contained 5 seedlings.…”

Section: Pathogenicity Testsmentioning

confidence: 99%

Role of riboflavin and thiamine in induced resistance against charcoal rot disease of soybean

Montaser¹

2011

Afr. J. Biotechnol.

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Charcoal rots (Macrophomina phaseolina (Tassi) Goidanich) of soybean (Glycine max (L.) Merr.) is a disease of economic significance throughout the world. Pathogenicity of 14 isolates of M. phaseolina was tested on soybean cv. Giza 21 under greenhouse conditions. The obtained data indicated that all the obtained isolates were able to attack soybean plants and caused charcoal rot on the basal stem with various degrees of diseases severity. M. phaseolina isolate S13 caused the highest charcoal rot severity (60%) followed by isolates S11 and S8 (57.9 and 56.3%, respectively). The effect of two inducer chemicals, that is, riboflavin (B2) and thiamine (B1) on the induction of systemic resistance in soybean against charcoal rot disease as well as biochemical changes associated with these treatments in soybean plants was investigated. Under greenhouse condition, the dose effect of 0.1 to 15 mM riboflavin and thiamine showed that 2.5 mM of riboflavin and 5 mM of thiamine was sufficient for maximum induction of resistance; higher concentration did not increase the effect. On the other hand, plants treated with riboflavin and thiamine and inoculated with pathogen grew higher than plants treated with sterilized distilled water (SDW) and inoculated with pathogen and increased fresh and dry weight of soybean plants. 10 mM concentration of riboflavin and thiamin recorded the highest dry and fresh weight compared with the control. In time course observation, it was observed that riboflavin and thiamine treated soybean plants induced resistance one day after treatment and reached its maximum level from 5 to 7 days in the case of riboflavin and 6 to 8 days in the case of thiamine and then decreased. Under field conditions, the percentage of damping-off, root rot and/or charcoal rot severity were significantly reduced due to soaking of the seeds in any of riboflavin and thiamine before sowing compared with the control treatment (seed treated with water) in both seasons (2008 to 2009 and 2009 to 2010). Also, these treatments significantly increased nodule numbers per plant, fresh and dry weight of nodules per plant compared with the control in both seasons. Generally, thiamine gave the best results in most cases under greenhouse and field conditions. In physiological studies, activity of defense-related enzymes, including peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), pathogenesis related (PR) protein (chitinase), were increased in the inoculated and non-inoculated plants treated with the thiamine and riboflavin respectively, compared with the control during the experimental period. In general, activity of these enzymes began to accumulate after two days of treatment and reached maximum levels at 8, 6, 8 and 8 days for PO, PPO, PAL and chitinase, respectively then the activities of these enzymes decreased progressively. On the other hand, total phenols and lignin increased in soybean plants inoculated with M. phaseolina and treated with thiamine and riboflavin. The highest accumulation of phenols was r...

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“…Catechol was used as a standard. The amount of phenolic content was expressed as phenol equivalents in mg/g fresh tissue [19].…”

Section: Determination Of Phenolic Compoundsmentioning

confidence: 99%

Induce Systemic Resistance against Root Rot and Wilt Diseases in Fodder Beet (Beta vulgaris L. var. rapacea Koch.) by Using Potassium Salts

Abdel-Monaim¹,

Atwa²

2015

J Plant Pathol Microbiol

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Role of Riboflavin in Induced Resistance against Fusarium Wilt and Charcoal Rot Diseases of Chickpea

Cited by 35 publications

References 28 publications

Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

Role of riboflavin and thiamine in induced resistance against charcoal rot disease of soybean

Induce Systemic Resistance against Root Rot and Wilt Diseases in Fodder Beet (Beta vulgaris L. var. rapacea Koch.) by Using Potassium Salts

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