Role of root and shoot tissues of excluders and hyperaccumulators in nickel transport and accumulation

Серегин, И. В.; Кожевникова, А. Д.; Давыдова, М. А.; Bystrova, E. I.; Ivanov, V. B.

doi:10.1134/s0012496607040138

Cited by 10 publications

(19 citation statements)

References 5 publications

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“…Once again, the data from the present study regarding the distribution of Ni in fresh, hydrated roots would tend to support this hypothesis, although further work is clearly required. Interestingly, this observation regarding the distribution of Ni is similar to that reported by Seregin et al [21] who studied several plant species exposed to toxic levels of Ni and reported that Ni often accumulates at the endodermis in non-hyperaccumulating plants. The 2D maps indicate that Zn uptake was greater than that of Ni, this result is in line with measured Zn concentrations reaching 1.1 µmol/g and Ni reaching 0.73 µmol/g (fresh root basis).…”

Section: Resultssupporting

confidence: 89%

Fast X-Ray Fluorescence Microtomography of Hydrated Biological Samples

et al. 2011

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Metals and metalloids play a key role in plant and other biological systems as some of them are essential to living organisms and all can be toxic at high concentrations. It is therefore important to understand how they are accumulated, complexed and transported within plants. In situ imaging of metal distribution at physiological relevant concentrations in highly hydrated biological systems is technically challenging. In the case of roots, this is mainly due to the possibility of artifacts arising during sample preparation such as cross sectioning. Synchrotron x-ray fluorescence microtomography has been used to obtain virtual cross sections of elemental distributions. However, traditionally this technique requires long data acquisition times. This has prohibited its application to highly hydrated biological samples which suffer both radiation damage and dehydration during extended analysis. However, recent advances in fast detectors coupled with powerful data acquisition approaches and suitable sample preparation methods can circumvent this problem. We demonstrate the heightened potential of this technique by imaging the distribution of nickel and zinc in hydrated plant roots. Although 3D tomography was still impeded by radiation damage, we successfully collected 2D tomograms of hydrated plant roots exposed to environmentally relevant metal concentrations for short periods of time. To our knowledge, this is the first published example of the possibilities offered by a new generation of fast fluorescence detectors to investigate metal and metalloid distribution in radiation-sensitive, biological samples.

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Section: Resultssupporting

confidence: 89%

Fast X-Ray Fluorescence Microtomography of Hydrated Biological Samples

et al. 2011

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“…1). These findings are in accordance with the results of Lombi et al (2011) who conducted tomography on a fresh, hydrated cowpea root exposed to 5 mM Ni for 24 h. Similarly, Seregin et al (2007) studied several plant species exposed to 10 to 400 mM Ni and reported that Ni moves readily into the root meristem, often accumulating at the endodermis in nonhyperaccumulators.…”

Section: Discussionsupporting

confidence: 80%

In Situ Distribution and Speciation of Toxic Copper, Nickel, and Zinc in Hydrated Roots of Cowpea

et al. 2011

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The phytotoxicity of trace metals is of global concern due to contamination of the landscape by human activities. Using synchrotron-based x-ray fluorescence microscopy and x-ray absorption spectroscopy, the distribution and speciation of copper (Cu), nickel (Ni), and zinc (Zn) was examined in situ using hydrated roots of cowpea (Vigna unguiculata) exposed to 1.5 μm Cu, 5 μm Ni, or 40 μm Zn for 1 to 24 h. After 24 h of exposure, most Cu was bound to polygalacturonic acid of the rhizodermis and outer cortex, suggesting that binding of Cu to walls of cells in the rhizodermis possibly contributes to the toxic effects of Cu. When exposed to Zn, cortical concentrations remained comparatively low with much of the Zn accumulating in the meristematic region and moving into the stele; approximately 60% to 85% of the total Zn stored as Zn phytate within 3 h of exposure. While Ni concentrations were high in both the cortex and meristem, concentrations in the stele were comparatively low. To our knowledge, this is the first report of the in situ distribution and speciation of Cu, Ni, and Zn in hydrated (and fresh) plant tissues, providing valuable information on the potential mechanisms by which they are toxic.

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“…Strontium was only observed in the surface cell layers, whereas Ni penetrated deep into the meristem [32,33]. Similar observations were reported for maize seedlings grown on the Hoagland solution [45]. The Ni distribution became increasingly nonuniform in the presence of Ca compared to the distribution pattern in the absence of Ca.…”

Section: Root Capsupporting

confidence: 60%

“…A substantial amount of Pb in spherical aggregates on the external side of the plasmalemma was also found in onion roots [81]. These results provide additional evidence for the existence of two pathways of Ni transport along the root cortical cells: through the protoplasts (i.e., symplast) and through the periplasmic space (i.e., apoplast) [12,36,45]. In plasmolyzed cells, Ni was observed inside both the protoplast and the periplasmic space.…”

Section: Primary Cortexmentioning

confidence: 76%

“…A question arises whether the exodermis performs the barrier function in the transport of heavy metals. In the case of Ni, the intense staining of exodermal cells in the basal root part was observed after 5 to 7 days of incubation, whereas the metal content in the cortical layer located under the exodermis was substantially lower [45]. Taking into account the limitation of vertical transport of heavy metals in the cortex, one may suppose that the metals are able to pass partially through the modified cell walls of the exodermis.…”

Section: Primary Cortexmentioning

confidence: 95%

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10.1007/s11183-008-1001-8

2010

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The review considers the roles of root and shoot tissues in transport and accumulation of heavy metals in plants of two contrast groups, i.e., excluders and hyperaccumulators. The regularities in distribution of cadmium, lead, nickel, and strontium are summarized. Effects of other cations, calcium in particular, on accumulation and distribution of heavy metals are analyzed. Specific patterns of metal distribution in hyperaccumulator plants are discussed together with morphological and functional features underlying the ability of plants to accumulate heavy metals in the aboveground organs. Based on the data available, the root and shoot tissues are classified according to their roles in transport and distribution of the metals examined.

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Role of root and shoot tissues of excluders and hyperaccumulators in nickel transport and accumulation

Cited by 10 publications

References 5 publications

Fast X-Ray Fluorescence Microtomography of Hydrated Biological Samples

Fast X-Ray Fluorescence Microtomography of Hydrated Biological Samples

In Situ Distribution and Speciation of Toxic Copper, Nickel, and Zinc in Hydrated Roots of Cowpea

10.1007/s11183-008-1001-8

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