Role of Salicylic Acid in Combating Heat Stress in Plants: Insights into Modulation of Vital Processes

Sangwan, Suman; Shameem, Nowsheen; Yashveer, Shikha; Tanwar, Hemender; Parray, Javid A.; Jatav, Hanuman Singh; Sharma, Sushma; Punia, Himani; Sayyed, R. Z.; Almalki, Waleed H.; Poczai, Péter

doi:10.31083/j.fbl2711310

Cited by 25 publications

(7 citation statements)

References 98 publications

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“…P19-specific upregulation of these genes is thus consistent with high accumulation and localization of the VSR in the cytosol. Induced HSP gene expression is also a mark of SA signaling (Snyman and Cronjé, 2008;Sangwan et al, 2022), consistent with enhanced SA accumulation and induction of SA response genes in P19 samples (Figure 7).…”

Section: Discussionsupporting

confidence: 61%

Molecular responses of agroinfiltratedNicotiana benthamianaleaves expressing suppressor of silencing P19 and influenza virus-like particles

Hamel,

Tardif,

Poirier-Gravel

et al. 2023

Preprint

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The production of influenza vaccines in plants is achieved through transient Agrobacterium-mediated expression of viral hemagglutinins (HAs). These proteins are produced and matured through the secretory pathway of plant cells, before being trafficked to the plasma membrane where they induce formation of virus-like particles (VLPs). Production of VLPs unavoidably impacts plant cells, as do viral suppressors of RNA silencing (VSRs) that are often co-expressed to increase protein yields. However, little information is available on host molecular responses to these foreign proteins. The present work provides a comprehensive overview of transcriptomic, metabolic, and signaling changes occurring in Nicotiana benthamiana leaf cells transiently expressing the VSR P19, or co-expressing P19 and an influenza HA. Our data identifies generic responses to Agrobacterium-mediated expression of foreign proteins, including shutdown of chloroplast gene expression, activation of oxidative stress responses, and reinforcement of the plant cell wall through lignification. Our results also indicate that P19 expression promotes salicylic acid (SA) signaling, a process apparently antagonized by co-expression of HA. As the latter induces specific signatures, with effects on lipid metabolism, lipid distribution, and oxylipin signaling, dampening of P19 responses suggests crosstalk between SA and oxylipin pathways. Consistent with the upregulation of oxidative stress-related genes and proteins, we finally show that reduction of oxidative stress damage through exogenous application of ascorbic acid improves plant biomass quality during production of VLPs.

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Section: Discussionsupporting

confidence: 61%

Molecular responses of agroinfiltratedNicotiana benthamianaleaves expressing suppressor of silencing P19 and influenza virus-like particles

Hamel,

Tardif,

Poirier-Gravel

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Upregulation of these genes in P19 samples was thus consistent with higher accumulation of the VSR (Figure 1c), and with its sub-cellular localization in the cytosol. Upregulation of HSP genes is also a well-known mark of SA signalling (Sangwan et al, 2022;Snyman and Cronj e, 2008), consistent with enhanced SA accumulation and induction of other SA response genes in P19 samples (Figure 7). Interestingly, no expression of HSP genes was seen in H5 samples (Figure 3), despite confirmed accumulation of the P19 protein in this condition (Figure 1c).…”

Section: Discussionsupporting

confidence: 71%

Molecular responses of agroinfiltrated Nicotiana benthamiana leaves expressing suppressor of silencing P19 and influenza virus‐like particles

Hamel,

Tardif,

Poirier‐Gravel

et al. 2023

Plant Biotechnology Journal

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SummaryThe production of influenza vaccines in plants is achieved through transient expression of viral hemagglutinins (HAs), a process mediated by the bacterial vector Agrobacterium tumefaciens. HA proteins are then produced and matured through the secretory pathway of plant cells, before being trafficked to the plasma membrane where they induce formation of virus‐like particles (VLPs). Production of VLPs unavoidably impacts plant cells, as do viral suppressors of RNA silencing (VSRs) that are co‐expressed to increase recombinant protein yields. However, little information is available on host molecular responses to foreign protein expression. This work provides a comprehensive overview of molecular changes occurring in Nicotiana benthamiana leaf cells transiently expressing the VSR P19, or co‐expressing P19 and an influenza HA. Our data identifies general responses to Agrobacterium‐mediated expression of foreign proteins, including shutdown of chloroplast gene expression, activation of oxidative stress responses and reinforcement of the plant cell wall through lignification. Our results also indicate that P19 expression promotes salicylic acid (SA) signalling, a process dampened by co‐expression of the HA protein. While reducing P19 level, HA expression also induces specific signatures, with effects on lipid metabolism, lipid distribution within membranes and oxylipin‐related signalling. When producing VLPs, dampening of P19 responses thus likely results from lower expression of the VSR, crosstalk between SA and oxylipin pathways, or a combination of both outcomes. Consistent with the upregulation of oxidative stress responses, we finally show that reduction of oxidative stress damage through exogenous application of ascorbic acid improves plant biomass quality during production of VLPs.

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“…Effect of SA and AsA on photosynthetic activity in heatstressed apple leaves: The positive effect of SA in plants and its significant role in preventing heat stress has already been reported (Sangwan et al 2022). Although some previous studies showed that SA inhibits photosynthetic activity, others found that photosynthesis increases when SA is present in small concentrations (Gao et al 2018, Janda et al 2014.…”

Section: Effect Of High Temperature On Photosynthetic Activity In App...mentioning

confidence: 94%

Photosynthetic responses of heat-stressed apple leaves to foliar application of salicylic and ascorbic acid

MIHALJEVIĆ,

VILJEVAC VULETIĆ,

TOMAŠ

et al. 2024

Photosynt.

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Role of Salicylic Acid in Combating Heat Stress in Plants: Insights into Modulation of Vital Processes

Cited by 25 publications

References 98 publications

Molecular responses of agroinfiltratedNicotiana benthamianaleaves expressing suppressor of silencing P19 and influenza virus-like particles

Molecular responses of agroinfiltratedNicotiana benthamianaleaves expressing suppressor of silencing P19 and influenza virus-like particles

Molecular responses of agroinfiltrated Nicotiana benthamiana leaves expressing suppressor of silencing P19 and influenza virus‐like particles

Photosynthetic responses of heat-stressed apple leaves to foliar application of salicylic and ascorbic acid

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