Role of salivary leukocyte protease inhibitor in periodontal disease progression

Abstract

Context:Proteases play a major role in the tissue destruction involved in periodontal disease. It is known that the balance between proteases and their inhibitors is a major determinant in maintaining tissue integrity. The association between the proteases and periodontitis is well established, but not many studies have been carried out to know the role played by a protease inhibitor like salivary leukocyte protease inhibitor (SLPI) in periodontitis.Aim:The aim of the present study was to correlate SLPI with periodontitis.Settings and Design:Case-control study.Materials and Methods:Seventy-five clinically confirmed cases of periodontitis and 20 controls were included in the study. A detailed case history and periodontal index (PI) were recorded. Two milliliters of unstimulated saliva samples was obtained and subjected to quantification of SLPI leaves using SLPI in enzyme-linked immunosorbent assay (ELISA) kit. Based on the periodontal index score of the individuals, the cases and controls were divided into groups A, B and C, and the obtained SLPI levels were compared among the groups.Statistical Analysis:Mann-Whitney U test and correlation coefficient test.Results:The results showed that in the initial stages of periodontitis there is a tendency of SLPI levels to be raised. The SLPI levels were found to be reduced in the terminal stages of periodontitis.Conclusion:It appears that SLPI accumulates in the local environment, at least in the initial stages of the periodontal disease, probably to inhibit the action of increased elastic activity.

Neutrophil elastase and endogenous inhibitors in Behçet's disease saliva

“…This suggests that SLPI is depleted in BDq patients despite being orally asymptomatic. A similar inverse NE/SLPI dynamic has been previously reported in active periodontitis saliva [38], and reduced SLPI has been associated with periodontal disease progression [39].…”

Salivary secretory leukocyte protease inhibitor levels in patients with stage 3 grade C periodontitis: a comparative cross-sectional study

“…To date, there is very limited data on the salivary SLPI levels in systemically healthy periodontitis patients and also about the diagnostic capacity of salivary levels of this inhibitor in periodontal diseases 20,21 . Therefore, in this study, whole saliva was used to evaluate SLPI levels of individuals with periodontitis.…”

“…Increased levels of neutrophile proteases (elastase, protease 3 and cathepsin B) can contribute to significant depletion of SLPI in the infected periodontitis sites in those patients 18,32,36 . Pateel et al 21 speculated that lower salivary SLPI levels in chronic periodontitis may be explained by the degradation of SLPI by cathepsins and bacterial cysteine proteases. Therefore, in the present study, when compared to periodontal health and gingivitis, reduced normalized salivary SLPI levels in generalized stage 3 grade C periodontitis might be due to the proteolytic cleavage of this inhibitor by P. gingivalis gingipain and neutrophil proteases.…”

“…To date, little is known about the SLPI levels in saliva of patients with different forms of periodontal disease 20,21 . Previously, Cox et al 20 demonstrated that SLPI is present in whole saliva of patients with severe chronic periodontitis and reported that the levels of SLPI in saliva were significantly lower than in GCF obtained from the same patients.…”

Efeito da dislipidemia sobre a capacidade de defesa - estudo experimental

“…Sua presença na região inflamada também está relacionada à sua ação antimicrobiana, anti-inflamatória e de cicatrização. No entanto, nas fases terminais da doença periodontal (destruição avançada, perda da função) o SLPI está reduzido, provavelmente devido à sua degradação pelas proteases catepsina L, cisteínas bacterianas (gingipains) e devido à interação na formação do complexo com elastase (Kantyka et al, 2009;Pateel et al, 2010).…”