Industries are moving towards finding a natural source of functionally active constituents which is better and safer materials to fulfill customers’ demand. Marine algae contain a huge variety of biologically active compounds and express a promising role in different applications. Hence, the present study was carried out to characterize different biochemicals from brown alga Sargassum tenerrimum by FTIR, GCMS, HRLCMS Q-TOF, and ICP AES technique. First, the macroalga was collected from the Beyt Dwarka sea site, the Western coast of Gujarat, India. In FTIR, different types of bioactive functional groups were characterized as accountable for different beneficial components whereas ethanolic and methanolic extracts of S. tenerrimum reported fourteen and nineteen different beneficial phycocompounds in GCMS analysis, respectively. In HRLCMS Q-TOF analysis, two compounds were found carbohydrate derivatives and fifty-nine different compounds were determined to be different types of phycocompounds. Additionally, in the ICP AES study, Silicon was found to be high up in amount whereas Copper remained the minimum among studied elements. Moreover, the HRLCMS-QTOF study of amino acids reported that Glutamic acid (132.13 nmol/mL) was found to be the highest whereas Isoleucine (0.70 nmol/mL) was found to be the least amidst measured twenty-one amino acids. Bioactive potentials of these compounds have been reported in many previous studies. The inclusion of macroalgae-derived constituents in different applications has been broadly rising due to their bioactive potentials.