Role of Serine/Threonine Phosphatase PP2A Class and Its Regulators in Salinity Stress Tolerance in Plants

Chawla, Srishti; Marothia, Deeksha; Pati, Pratap Kumar

doi:10.1007/978-3-030-48733-1_4

Cited by 3 publications

(3 citation statements)

References 90 publications

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“…Among the key down-regulated genes included Serine/threonine-protein phosphatase. The serine/ threonine phosphatase PP2A family plays an important role in regulating protein dephosphorylation in a highly coordinated manner in association with other regulatory subunits (Chawla et al 2020). Arabidopsis plants with mutations in Protein phosphatase 2A exhibited late flowering compared to wild type (Kataya et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of almond (Prunus dulcis) cultivars with differential flowering time

Mir,

Raina,

Sharma

et al. 2024

IJGPB

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Almond is one of the oldest and major tree nut crops consumed for its nutritious kernels. Since transcriptome analysis is crucial to identifying genes controlling important agronomic, an attempt was made for comparative transcriptomic analysis to identify the genetic differences in almond cultivars Waris and Ferralise, varying in blooming dates. Sequencing data from Illumina TruSeq libraries was processed using the FASTQC-Trimmomatic-HISAT2-Stringtie-Stringtie merge-DESeq2 pipeline. Overall alignment rates for different libraries ranged from 82.04-91.33%. DESeq2 analysis identified 3707 differentially expressed genes, out of which 1558 genes were up-regulated while the 2149 genes were down-regulated in Waris compared to Ferralise. Differential regulation of genes associated with the auxin signaling pathway, ubiquitin ligases, serine/threonine-protein phosphatases, proteasomes, translation initiation factors, phosphatidylinositol signaling and pentose phosphate pathway provides new insights into the molecular regulation of blooming in almonds. These findings will pave the way for molecularly differentiating early and late flowering almond cultivars and have implications in breeding late-blooming almond cultivars

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of almond (Prunus dulcis) cultivars with differential flowering time

Mir,

Raina,

Sharma

et al. 2024

IJGPB

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show abstract

“…Previous research has reported that protein kinases (PKs) and protein phosphatases (PPs) play important roles in determining the magnitude and duration of a signaling event, with PKs catalyzing the transfer of a phosphate moiety from ATP to proteins and PPs acting to remove this phosphate group by hydrolysis [ 36 ]. The protein serine/threonine phosphatases family from plants constitute PP1, PP2A, PP2B, and novel phosphatases, which have multiple biological functions by regulating a wide variety of cellular signal transduction pathways in response to stresses [ 37 ]. The RAD23 is involved in cell cycle regulation, protein quality control, DNA damage response, and cellular metabolism [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomics Reveals the Difference in Root Cold Resistance between Vitis. riparia × V. labrusca and Cabernet Sauvignon in Response to Freezing Temperature

Chen

Xing

et al. 2022

Plants

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Grapevines, bearing fruit containing large amounts of bioactive metabolites that offer health benefits, are widely cultivated around the world. However, the cold damage incurred when grown outside in extremely low temperatures during the overwintering stage limits the expansion of production. Although the morphological, biochemical, and molecular levels in different Vitis species exposed to different temperatures have been investigated, differential expression of proteins in roots is still limited. Here, the roots of cold-resistant (Vitis. riparia × V. labrusca, T1) and cold-sensitive varieties (Cabernet Sauvignon, T3) at −4 °C, and also at −15 °C for the former (T2), were measured by iTRAQ-based proteomic analysis. Expression levels of genes encoding candidate proteins were validated by qRT-PCR, and the root activities during different treatments were determined using a triphenyl tetrazolium chloride method. The results show that the root activity of the cold-resistant variety was greater than that of the cold-sensitive variety, and it declined with the decrease in temperature. A total of 25 proteins were differentially co-expressed in T2 vs. T1 and T1 vs. T3, and these proteins were involved in stress response, bio-signaling, metabolism, energy, and translation. The relative expression levels of the 13 selected genes were consistent with their fold-change values of proteins. The signature translation patterns for the roots during spatio-temporal treatments of different varieties at different temperatures provide insight into the differential mechanisms of cold resistance of grapevine.

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“…For the DEPs involved in bio-signaling, both protein kinases (PKs) and protein phosphatases (PPs) play important roles in determining the magnitude and duration of a signaling event, with PKs catalyzing the transfer of a phosphate moiety from ATP to proteins and PPs acting to remove this phosphate group by hydrolysis [36]. Protein serine/threonine phosphatases family from plants constitute PP1, PP2A, PP2B, and novel phosphatases, which have multiple biological functions by regulating a wide variety of cellular signal transduction pathways in response to stresses [37]. The RAD23 is involved in cell cycle regulation, protein quality control, DNA damage response and cellular metabolism [38].…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomics Reveals Differential Mechanism of Root Cold-resistance between Vitis. Riparia × V. Labrusca and Cabernet Sauvignon

Chen

Xing

et al. 2022

Preprint

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Grapevines, containing large amounts of bioactive metabolites that offer health benefits, are widely cultivated around the world. The cold damage of growing outside with extreme low temperature during overwintering stage limits the expansion of production. Although the levels of morphological, biochemical and molecular in different Vitis species exposure to different temperatures have been investigated, differential expression of proteins in roots is still limited. Here, the roots of cold-resistant (Vitis. riparia × V. labrusca, T1) and cold-sensitive varieties (Cabernet Sauvignon, T3) at −4°C as well as of the former at −15°C (T2) were measured by iTRAQ-based proteomic analysis, expression levels of genes encoding candidate proteins were validated by qRT-PCR. The results showed that the root activity of cold-resistant variety was stronger than that of cold-sensitive variety, and it declined with the decrease of temperature. A total of 25 proteins were differentially co-expressed at T2 versus (vs) T1 and T1 vs T3, and these proteins were involved in stress response (e.g. DHN1, SHSPCP and USPCP), bio-signaling (e.g. PKCP, S/TPP and nsS/TP), metabolism (e.g. GluP, GluBE and PE), energy (e.g. AAC, AAACP and NADCP), and translation (e.g. rpL14, rpS21 and PPI). The relative expression levels of the candidate 13 genes were consistent with their fold-change values of proteins. The signature translation pattern for the roots at spatio-temporal treatments of varieties and temperatures provides insight into the differential mechanism of cold resistance of grapevines.

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Role of Serine/Threonine Phosphatase PP2A Class and Its Regulators in Salinity Stress Tolerance in Plants

Cited by 3 publications

References 90 publications

Transcriptomic analysis of almond (Prunus dulcis) cultivars with differential flowering time

Transcriptomic analysis of almond (Prunus dulcis) cultivars with differential flowering time

Comparative Proteomics Reveals the Difference in Root Cold Resistance between Vitis. riparia × V. labrusca and Cabernet Sauvignon in Response to Freezing Temperature

Comparative Proteomics Reveals Differential Mechanism of Root Cold-resistance between Vitis. Riparia × V. Labrusca and Cabernet Sauvignon

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