Role of serotonin 4 receptor in the growth of hippocampal neurons during the embryonic development in mice

Agrawal, Lokesh; Vimal, Sunil Kumar; Shiga, Takashi

doi:10.1016/j.neuropharm.2019.107712

Cited by 17 publications

(6 citation statements)

References 77 publications

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“…While the brain is formed, GABA does not only serve short-term communication, but it also triggers synaptogenesis (Oh et al 2016), synapse pruning (Wu et al 2012), neuronal migration (Li et al 2018), stem cell fate (Song et al 2012) and neurogenesis (Kriegstein 2005;Tozuka et al 2005). Other neurotransmitters take similar roles, such as spine growth triggered by glutamate (Kwon and Sabatini 2011), and various neurogenic processes affected by serotonin (Schaefer et al 2013;Migliarini et al 2013;Agrawal et al 2019), or nicotine (Slikker Jr et al 2005;Dwyer et al 2009;Slotkin et al 2016;Zahedi et al 2019). The toxicological consequence is that disturbance of neurotransmitter signaling during development can result in an altered brain connectivity in later life.…”

Section: Introductionmentioning

confidence: 99%

Functional alterations by a subgroup of neonicotinoid pesticides in human dopaminergic neurons

et al. 2021

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Neonicotinoid pesticides, originally developed to target the insect nervous system, have been reported to interact with human receptors and to activate rodent neurons. Therefore, we evaluated in how far these compounds may trigger signaling in human neurons, and thus, affect the human adult or developing nervous system. We used SH-SY5Y neuroblastoma cells as established model of nicotinic acetylcholine receptor (nAChR) signaling. In parallel, we profiled dopaminergic neurons, generated from LUHMES neuronal precursor cells, as novel system to study nAChR activation in human post-mitotic neurons. Changes of the free intracellular Ca2+ concentration ([Ca2+]i) were used as readout, and key findings were confirmed by patch clamp recordings. Nicotine triggered typical neuronal signaling responses that were blocked by antagonists, such as tubocurarine and mecamylamine. Pharmacological approaches suggested a functional expression of α7 and non-α7 nAChRs on LUHMES cells. In this novel test system, the neonicotinoids acetamiprid, imidacloprid, clothianidin and thiacloprid, but not thiamethoxam and dinotefuran, triggered [Ca2+]i signaling at 10–100 µM. Strong synergy of the active neonicotinoids (at low micromolar concentrations) with the α7 nAChR-positive allosteric modulator PNU-120596 was observed in LUHMES and SH-SY5Y cells, and specific antagonists fully inhibited such signaling. To provide a third line of evidence for neonicotinoid signaling via nAChR, we studied cross-desensitization: pretreatment of LUHMES and SH-SY5Y cells with active neonicotinoids (at 1–10 µM) blunted the signaling response of nicotine. The pesticides (at 3–30 µM) also blunted the response to the non-α7 agonist ABT 594 in LUHMES cells. These data show that human neuronal cells are functionally affected by low micromolar concentrations of several neonicotinoids. An effect of such signals on nervous system development is a toxicological concern.

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Section: Introductionmentioning

confidence: 99%

Functional alterations by a subgroup of neonicotinoid pesticides in human dopaminergic neurons

et al. 2021

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“… 70 A previous study confirmed that CRMP2-mediated neuroprotection and microtubule dynamics are increased in a resilience model of acute depression-like behaviours. 71 Some studies 72 have suggested a pathway that may interlink the activity of the serotonin 4 receptor (5-HT4R) with CRMP2 expression and dephosphorylation through elevated mRNA expression of brain-derived neurotrophic factor (BDNF) via 5-HT4R. Upregulated expression of neurotrophic factors, including BDNF, inhibits the phosphorylation of CRMP2 protein and increases CRMP2 levels, which promotes the growth of axons and dendrites.…”

Section: Discussionmentioning

confidence: 99%

Brief pup separation during lactation confers resilience in behavioural deficits induced by chronic restraint stress in postpartum C57BL/6J dams

Zhou,

Wu,

Zhao

et al. 2023

jpn

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Background: The postpartum period is a complex time for females that affects health recovery. Stress during this period is one of the main risk factors for depression. Therefore, preventing stress-induced depression in the postpartum period is of great importance. Pup separation (PS) is a natural paradigm of postpartum care; however, the effect of different PS protocols during lactation on stress-induced depressive behaviours in dams is unknown. Methods: Lactating C57BL/6J mice were subjected to no pup separation (NPS), brief PS (15 min/day, PS15) or long PS (180 min/day, PS180) from postpartum day 1 to postpartum day 21 and were then subjected to chronic restraint stress (CRS) for 21 days. Behavioural tests, specifically the open field test (OFT), elevated plus maze (EPM) test and tail suspension test (TST), were performed. The expression of mRNA and protein in the hippocampus and microbiota composition were also assessed. Results: We observed CRS-induced anxiety- and depression-like behaviours in NPS dams. In addition, in NPS dams, microglial activation and the levels of NOD-like receptor pyrin domain containing 3, caspase-1 and interleukin-1β were increased, whereas expression levels of collapsing response mediator protein 2 (CRMP2) and α-tubulin were decreased. However, immobility time in the TST was lower in PS15+CRS dams than in NPS+CRS dams, and time spent in the centre during the OFT and in the open arms during the EPM test was higher in PS15+CRS dams, indicating resilience. Expression of hippocampal biomarkers of neuroinflammation was inhibited and levels of CRMP2-mediated neuroplasticity were increased in PS15+CRS dams. Notably, we observed taxonomic changes in the cecal microbiota across different PS groups, as well as relationships between gut microbiota composition and some biomarkers of hippocampal neuroinflammation and neuroplasticity. Limitations: The sample size for gut microbiota analysis in this study was small. Conclusion: Collectively, the results of this study confirm that brief PS confers stress resilience in CRS-induced behavioural deficits and reverses hippocampal neuroinflammation–neuroplasticity injury and gut microbiota imbalance.

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“…On the other hand, the Htr2, Htr3, Htr4, Htr6, and Htr7 receptor families are excitatory. Furthermore, the stimulation of 5-HT4R with the agonist RS67333 had increased hippocampal neurons dendritic growth ( Agrawal et al, 2019 ) as it upregulates the expression of the neurotrophic factors BDNF, NT-3, NGF as also the TRK-A receptor. Moreover, Htr3 is ionotropic and could depolarize the plasma membrane, resulting in cations influx.…”

Section: Extracellular Molecular Signals Regulating Dendritic Growth:...mentioning

confidence: 99%

Extracellular molecular signals shaping dendrite architecture during brain development

Hamad,

Emerald,

Kumar

et al. 2023

Front. Cell Dev. Biol.

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Proper growth and branching of dendrites are crucial for adequate central nervous system (CNS) functioning. The neuronal dendritic geometry determines the mode and quality of information processing. Any defects in dendrite development will disrupt neuronal circuit formation, affecting brain function. Besides cell-intrinsic programmes, extrinsic factors regulate various aspects of dendritic development. Among these extrinsic factors are extracellular molecular signals which can shape the dendrite architecture during early development. This review will focus on extrinsic factors regulating dendritic growth during early neuronal development, including neurotransmitters, neurotrophins, extracellular matrix proteins, contact-mediated ligands, and secreted and diffusible cues. How these extracellular molecular signals contribute to dendritic growth has been investigated in developing nervous systems using different species, different areas within the CNS, and different neuronal types. The response of the dendritic tree to these extracellular molecular signals can result in growth-promoting or growth-limiting effects, and it depends on the receptor subtype, receptor quantity, receptor efficiency, the animal model used, the developmental time windows, and finally, the targeted signal cascade. This article reviews our current understanding of the role of various extracellular signals in the establishment of the architecture of the dendrites.

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Role of serotonin 4 receptor in the growth of hippocampal neurons during the embryonic development in mice

Cited by 17 publications

References 77 publications

Functional alterations by a subgroup of neonicotinoid pesticides in human dopaminergic neurons

Functional alterations by a subgroup of neonicotinoid pesticides in human dopaminergic neurons

Brief pup separation during lactation confers resilience in behavioural deficits induced by chronic restraint stress in postpartum C57BL/6J dams

Extracellular molecular signals shaping dendrite architecture during brain development

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