Role of Serum cartilage oligomeric matrix protein (COMP) in the diagnosis of rheumatoid arthritis (RA): A case–control study

Liu, Fengxia; Wang, Xijuan; Zhang, Xude; Ren, Chao; Xiao, Jie

doi:10.1177/0300060516639504

Cited by 13 publications

(12 citation statements)

References 28 publications

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“… 28 The s COMP level also has the potential to be used as a biological marker for differentiating between patients with rheumatoid arthritis (RA) and healthy individuals. 29 , 30 The relevance of COMP and bone development or disease has been identified by previous studies. 31 , 32 Based on our study results, it is necessary to further explore the potential of COMP in the diagnosis and treatment of KBD.…”

Section: Discussionmentioning

confidence: 89%

The molecular mechanism study of COMP involved in the articular cartilage damage of Kashin-Beck disease

Liang

Wang

et al. 2020

Bone & Joint Research

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Aims Kashin-Beck disease (KBD) is a kind of chronic osteochondropathy, thought to be caused by environmental risk factors such as T-2 toxin. However, the exact aetiology of KBD remains unclear. In this study, we explored the functional relevance and biological mechanism of cartilage oligosaccharide matrix protein (COMP) in the articular cartilage damage of KBD. Methods The articular cartilage specimens were collected from five KBD patients and five control subjects for cell culture. The messenger RNA (mRNA) and protein expression levels were detected by quantitative reverse transcription PCR (qRT-PCR) and western blot. The survival rate of C28/I2 chondrocyte cell line was detected by MTT assay after T-2 toxin intervention. The cell viability and mRNA expression levels of apoptosis related genes between COMP-overexpression groups and control groups were examined after cell transfection. Results The mRNA and protein expression levels of COMP were significantly lower in KBD chondrocytes than control chondrocytes. After the T-2 toxin intervention, the COMP mRNA expression of C28/I2 chondrocyte reduced and the protein level of COMP in three intervention groups was significantly lower than in the control group. MTT assay showed that the survival rate of COMP overexpression KBD chondrocytes were notably higher than in the blank control group. The mRNA expression levels of Survivin, SOX9, Caspase-3, and type II collagen were also significantly different among COMP overexpression, negative control, and blank control groups. Conclusion Our study results confirmed the functional relevance of COMP with KBD. COMP may play an important role in the excessive chondrocytes apoptosis of KBD patients. Cite this article: Bone Joint Res 2020;9(9):578–586.

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Section: Discussionmentioning

confidence: 89%

The molecular mechanism study of COMP involved in the articular cartilage damage of Kashin-Beck disease

Liang

Wang

et al. 2020

Bone & Joint Research

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“…As a cartilage-derived marker of cartilage breakdown, COMP is undoubtedly considered as a prognostic factor in RA, especially at early stage. For instance, serum COMP level is signi cantly higher in patients with RA compared with control subjects, and its predictive accuracy is much higher than elevated ACPA level as a biomarker [11] .…”

Section: Discussionmentioning

confidence: 99%

Systemic Proteomic Analysis Reveals Distinct Exosomal Proteins Profiles in Rheumatoid Arthritis

Qin¹,

Song²,

Du³

et al. 2020

Preprint

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BackgroundRheumatoid arthritis (RA) is a heterogeneous and complex disease characterized by autoantibodies production and inflammation of the synovium. Its underlying mechanisms remain elusive. In recent years, exosomes have emerged as non-invasive biomarkers in diverse diseases. Due to its various inflammatory and immunological functions, some studies highlighted abnormalities of exosomes in RA, but fewer have paid attention to the broad spectrum of potential systemic markers of RA. The aim of this study was to identify exosomal candidate proteins in the pathogenesis of RA using systemic proteomic method. MethodsTotally 12 specimens of plasma from 6 RA patients and 6 age-and gender-matched controls from the Chinese population were obtained for nanoscale liquid chromatography coupled to tandem mass spectrometry (nano-LC-MS/MS) analysis to identify differentially expressed proteins (DEPs) in exosomes. Gene ontology (GO), protein interaction network (PPI) and KEGG pathway analyses were used in subcellular localization analysis. ResultsA total of 278 exosomal proteins were detected. Among them, 32 proteins were significantly upregulated (FC≥2.0 and P<0.05) and 5 proteins were downregulated (FC≤0.5 and P<0.05). Most of these DEPs were immunoglobullins (Igs). Bioinformatics analysis indicated that these Igs are involved in inflammatory response of RA through diverse immune regulatory pathways, such as NF-kappa B signaling pathway. PPI analysis revealed that transthyretin (TTR, P02766), angiotensinogen (AGT, P01019), lipopolysaccharide-binding protein (LBP, P18428), monocyte differentiation antigen CD14 (CD14, P08571), cartilage oligomeric matrix protein (COMP, P49747), serum amyloid P (SAP/APCS, P02743) and tenascin (TNC, P24821) can interact with each other or may form a regulatory networks. Subsequently, these cross-linked proteins may mainly involve in the NF-kappa B signaling pathway or inflammatory-related pathways to mediate the onset of RA. Noteworthy, among these exosomal DEPs, the complex composed of LBP and CD14 is involved in NF-kappa B signaling pathway, resulting in promoted expression of IL-8, TNF-α, and eventually leading to the development of RA.ConclusionsOur findings suggest distinct plasmic exosomal protein profiles in RA patients. These proteins not only take important parts in the vicious circle in the pathogenic process of RA, but also serve as novel biomarkers in RA diagnosis and prognosis.

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“…Serum cartilage oligomeric matrix protein (COMP) has been found to be a strong predictive biomarker for response to abatacept treatment in RA [103]. Furthermore, serum COMP level correlated to disease activity of RA [104] and had superior sensitivity, specificity, and accuracy for diagnosis of RA [105]. Serum collagen type I (TXI) was also associated with RA disease activity [106].…”

Section: Utility Of Chondrocyte Products As Diagnostic and Prognosticmentioning

confidence: 99%

Dual Role of Chondrocytes in Rheumatoid Arthritis: The Chicken and the Egg

Tseng

Chen

Chang

et al. 2020

IJMS

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Rheumatoid arthritis (RA) is one of the inflammatory joint diseases that display features of articular cartilage destruction. The underlying disturbance results from immune dysregulation that directly and indirectly influence chondrocyte physiology. In the last years, significant evidence inferred from studies in vitro and in the animal model offered a more holistic vision of chondrocytes in RA. Chondrocytes, despite being one of injured cells in RA, also undergo molecular alterations to actively participate in inflammation and matrix destruction in the human rheumatoid joint. This review covers current knowledge about the specific cellular and biochemical mechanisms that account for the chondrocyte signatures of RA and its potential applications for diagnosis and prognosis in RA.

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Role of Serum cartilage oligomeric matrix protein (COMP) in the diagnosis of rheumatoid arthritis (RA): A case–control study

Cited by 13 publications

References 28 publications

The molecular mechanism study of COMP involved in the articular cartilage damage of Kashin-Beck disease

The molecular mechanism study of COMP involved in the articular cartilage damage of Kashin-Beck disease

Systemic Proteomic Analysis Reveals Distinct Exosomal Proteins Profiles in Rheumatoid Arthritis

Dual Role of Chondrocytes in Rheumatoid Arthritis: The Chicken and the Egg

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