Role of serum myeloperoxidase, CPK, CK-MB, and cTnI tests in early diagnosis of myocardial ischemia during ERCP

Yıldız, Kemalettin; İnce, Ali Tüzün; Kemik, Ahu Sarbay; Sert, Selin; Isen, Hayati Can; Tozlu, Mukaddes; Baysal, Birol; Akyüz, Ümit; Kocaman, Orhan; Danalıoğlu, Ahmet

doi:10.5152/tjg.2014.4716

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…CK-MB and cTnI are two important indicators for the dysfunction of myocardial cells and are widely used in the diagnosis of acute myocardial infarction. 18,19 We found that the expressions of CK-MB and cTnI in H9c2 myocardial cells were significantly elevated by stimulation with LPS, indicating that the dysfunction of myocardial cells was induced by LPS. The LPS-induced dysfunction of myocardial cells was dramatically alleviated by dulaglutide, indicating a promising protective effect of dulaglutide against sepsisinduced myocardial dysfunction.…”

Section: ■ Discussionmentioning

confidence: 79%

Dulaglutide Alleviates LPS-Induced Injury in Cardiomyocytes

Wang

Han

et al. 2021

ACS Omega

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Background and purpose: Sepsis is a severe infection-induced disease with multiple organ failure, and sepsis-induced cardiomyopathy is a fatal condition. Inflammatory response and oxidative stress are reported to be involved in the development of sepsis-induced cardiomyopathy. Dulaglutide is a novel antidiabetic agent that is currently reported to exert an anti-inflammatory effect. The present study aims to explore the potential protective property of dulaglutide on lipopolysaccharide (LPS)-induced injury on cardiomyocytes. Methods: LPS was used to induce an in vitro injury model on cardiomyocytes. The mitochondrial reactive oxygen species (ROS) level was detected using MitoSOX red, and reduced glutathione (GSH) was measured to evaluate the status of oxidative stress in H9c2 myocardial cells. The expressions of NADPH oxidase-1 (NOX-1) and inducible nitric oxidesynthase (iNOS) were determined using real-time PCR and western blot analysis. Real-time PCR and enzyme-linked immunosorbent assay (ELISA) were both used to detect the expressions and concentrations of tumor necrosis factor-α, interleukin-1β, interleukin-17, matrix metalloproteinase-2, and matrix metalloproteinase-9 in H9c2 myocardial cells, respectively. The production of nitric oxide (NO) was measured using the Griess reagent. The levels of creatine kinase isoenzyme-MB (CK-MB) and cardiac troponin I (cTnI) were detected using ELISA. Western blot was utilized to determine the expressions of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), and p-NF-κB p65 in H9c2 myocardial cells in the nucleus. Results: First, dulaglutide ameliorated LPS-induced oxidative stress by suppressing the production of mitochondrial ROS and elevating the level of reduced GSH, as well as downregulating NOX-1. Second, the LPS-induced cardiomyocyte injury was alleviated by dulaglutide through downregulating CK-MB and cTnI, accompanied by inhibiting iNOS expression and NO production. Lastly, the production of inflammatory factors and upregulation of MMPs induced by LPS were both significantly reversed by dulaglutide through suppressing the TLR4/Myd88/NF-κB signaling pathway. Conclusions: Dulaglutide alleviated LPS-induced injury in cardiomyocytes by inhibiting inflammation and oxidative stress.

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Section: ■ Discussionmentioning

confidence: 79%

Dulaglutide Alleviates LPS-Induced Injury in Cardiomyocytes

Wang

Han

et al. 2021

ACS Omega

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“…CK‐MB and cTnI are classic markers of cardiac injury that are mainly distributed in the myocardium and rapidly released in large amounts in the blood when myocardial injury occurs (Yildiz et al, 2014). The results of the Elisa assay for CK‐MB and cTnI in serum showed that both were significantly lower in the LT group than in the SA group (Figure 3), and cTnI levels were significantly higher in the IBA group as compared with the LT group.…”

Section: Discussionmentioning

confidence: 99%

Integrated transcriptomics and metabolomics reveal protective effects on heart of hibernating Daurian ground squirrels

Yang,

Hao,

et al. 2023

Journal Cellular Physiology

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Hibernating mammals are natural models of resistance to ischemia, hypoxia‐reperfusion injury, and hypothermia. Daurian ground squirrels (spermophilus dauricus) can adapt to endure multiple torpor‐arousal cycles without sustaining cardiac damage. However, the molecular regulatory mechanisms that underlie this adaptive response are not yet fully understood. This study investigates morphological, functional, genetic, and metabolic changes that occur in the heart of ground squirrels in three groups: summer active (SA), late torpor (LT), and interbout arousal (IBA). Morphological and functional changes in the heart were measured using hematoxylin‐eosin (HE) staining, Masson staining, echocardiography, and enzyme‐linked immunosorbent assay (ELISA). Results showed significant changes in cardiac function in the LT group as compared with SA or IBA groups, but no irreversible damage occurred. To understand the molecular mechanisms underlying these phenotypic changes, transcriptomic and metabolomic analyses were conducted to assess differential changes in gene expression and metabolite levels in the three groups of ground squirrels, with a focus on GO and KEGG pathway analysis. Transcriptomic analysis showed that differentially expressed genes were involved in the remodeling of cytoskeletal proteins, reduction in protein synthesis, and downregulation of the ubiquitin‐proteasome pathway during hibernation (including LT and IBA groups), as compared with the SA group. Metabolomic analysis revealed increased free amino acids, activation of the glutathione antioxidant system, altered cardiac fatty acid metabolic preferences, and enhanced pentose phosphate pathway activity during hibernation as compared with the SA group. Combining the transcriptomic and metabolomic data, active mitochondrial oxidative phosphorylation and creatine‐phosphocreatine energy shuttle systems were observed, as well as inhibition of ferroptosis signaling pathways during hibernation as compared with the SA group. In conclusion, these results provide new insights into cardio‐protection in hibernators from the perspective of gene and metabolite changes and deepen our understanding of adaptive cardio‐protection mechanisms in mammalian hibernators.

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“…From this, we obtained that sepsis fundamentally affected the systolic and diastolic functions of the myocardium. cTnI and CK-MB are commonly used in clinical evaluation of myocardial status and are specific markers of myocardial injury [ 29 , 30 ]. We noticed that the levels of cTnI and CK-MB were increased in the serum of CLP group, suggesting the cardiac dysfunction of sepsis rat was accompanied by the occurrence of myocardial injury.…”

Section: Discussionmentioning

confidence: 99%