Role of serum on cryopreservation and subsequent viability of mouse bone marrow hemopoietic stem cells

Grilli, G; Porcellini, A; Lucarelli, G

doi:10.1016/0011-2240(80)90063-2

Cited by 32 publications

(16 citation statements)

References 8 publications

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“…Previous work has shown that serum is a useful additive for increasing the cryopreservation efficiency of various cells and tissues [45, 46]. However, serum is not suitable for human clinical applications because of its lack of definition and potential to carry harmful agents such as viruses [12, 47].…”

Section: Discussionmentioning

confidence: 99%

Effect of Antioxidants and Apoptosis Inhibitors on Cryopreservation of Murine Germ Cells Enriched for Spermatogonial Stem Cells

Kim

Lee

et al. 2016

PLoS ONE

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Spermatogonial stem cells (SSCs) are germline stem cells that serve as the foundation of spermatogenesis to maintain fertility throughout a male’s lifetime. To treat male infertility using stem cell banking systems and transplantation, it is important to be able to preserve SSCs for long periods of time. Therefore, this study was conducted to develop an optimal cryopreservation protocol for SSCs using antioxidants and apoptosis inhibitors in freezing medium. No differences were observed compared to controls when SSCs were cryopreserved in the presence of apoptosis inhibitors by themselves. However, mouse germ cells cryopreserved in basal medium containing the antioxidant hypotaurine (14 mM) resulted in significantly greater proliferation potential and mitochondrial activity. Furthermore, treatment groups with combinations containing 200 mM trehalose and 14 mM hypotaurine showed higher proliferation rates compared to controls. In addition, several serum free conditions were evaluated for SSC cryopreservation. Treatment media containing 10% or 20% knockout serum replacement resulted in similar cryopreservation results compared to media containing FBS. SSC transplantation was also performed to confirm the functionality of SSCs frozen in 14 mM hypotaurine. Donor SSCs formed normal spermatogenic colonies and sperm in the recipient testis. These data indicate that inclusion of 14 mM hypotaurine in cryopreservation media is an effective way to efficiently cryopreserve germ cells enriched for SSCs and that knockout serum replacement can replace FBS in germ cell cryopreservation media.

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Section: Discussionmentioning

confidence: 99%

Effect of Antioxidants and Apoptosis Inhibitors on Cryopreservation of Murine Germ Cells Enriched for Spermatogonial Stem Cells

Kim

Lee

et al. 2016

PLoS ONE

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“…On the other hand, the usefulness of serum as a component of storage media was emphasized by Grilli et al 20 in the cryopreservation of bone marrow cells. They confirmed that homologous serum is required for optimal stem cell cryopreservation, because macromolecules such as serum proteins can protect stem cells against handling injury during the actual freezing-thawing process.…”

Section: Discussionmentioning

confidence: 99%

Successful liquid storage of peripheral blood stem cells at subzero non-freezing temperature

Matsumoto

Yoshizawa

Kagamu

et al. 2002

Bone Marrow Transplant

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Summary:Although non-frozen storage of peripheral blood stem cells (PBSC) has been extensively studied and utilized clinically, the optimal storage conditions have not been determined. In order to improve the maintenance of clonogenic capacity during storage, we evaluated the feasibility of subzero non-freezing preservation of PBSC and attempted to determine the optimal conditions. Human PBSC were stored in different noncryopreserved conditions. University of Wisconsin (UW) solution was used as the storage medium for PBSC. The stem cell integrity was optimally maintained when PBSC were preserved in a supercooled state at ؊2؇C in UW solution without any cryoprotectants, and the highest values for nucleated cell survival (91.6%), CFU-GM survival (67.3%) and trypan blue viability (92%) were achieved at 72 h. CFU-GM survival in our storage conditions was significantly better than the survival achieved with hypothermic preservation in autologous serum and ACD-A solution at 4؇C (67.3 ؎ 9.2% vs 42.9 ؎ 15.3%; P Ͻ 0.01) or cryopreservation at ؊80؇C (67.3 ؎ 9.2% vs 52.7 ؎ 10.7%; P Ͻ 0.01). Thus, the combination of supercooling and UW solution was the optimal non-freezing method of preserving transplantable PBSC tested here. This method is of clinical utility in peripheral blood stem cell transplantation (PBSCT) for its simplicity and storage efficiency, and has value as a short-term storage method for PBSC to support doseintensive multicyclic chemotherapy.

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“…SSCs are presumed to have a comparable size and nucleus/cytoplasm ratio to lymphocytes, for which an optimal cooling rate of 10°C/min has already been defined (Thorpe et al ., 1976; Frederickx et al ., 2004). However, development of a cryopreservation method specific for purified bovine type A spermatogonia resulted in enhanced survival rates when using DMSO and an uncontrolled cooling rate of 1°C/min, which is the optimal rate for hematopoietic stem cells (Grilli et al ., 1980; Donaldson et al ., 1996; Izadyar et al ., 2002b). This study highlighted the advantage of an uncontrolled-rate freezing to the non-linear cooling rate (Izadyar et al ., 2002b).…”

Section: Resultsmentioning

confidence: 99%

Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation

Onofre

Baert

Faes

et al. 2016

Hum. Reprod. Update

167

116

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BACKGROUNDGerm cell depletion caused by chemical or physical toxicity, disease or genetic predisposition can occur at any age. Although semen cryopreservation is the first reflex for preserving male fertility, this cannot help out prepubertal boys. Yet, these boys do have spermatogonial stem cells (SSCs) that able to produce sperm at the start of puberty, which allows them to safeguard their fertility through testicular tissue (TT) cryopreservation. SSC transplantation (SSCT), TT grafting and recent advances in in vitro spermatogenesis have opened new possibilities to restore fertility in humans. However, these techniques are still at a research stage and their efficiency depends on the amount of SSCs available for fertility restoration. Therefore, maintaining the number of SSCs is a critical step in human fertility preservation. Standardizing a successful cryopreservation method for TT and testicular cell suspensions (TCSs) is most important before any clinical application of fertility restoration could be successful.OBJECTIVE AND RATIONALEThis review gives an overview of existing cryopreservation protocols used in different animal models and humans. Cell recovery, cell viability, tissue integrity and functional assays are taken into account. Additionally, biosafety and current perspectives in male fertility preservation are discussed.SEARCH METHODSAn extensive PubMED and MEDline database search was conducted. Relevant studies linked to the topic were identified by the search terms: cryopreservation, male fertility preservation, (immature)testicular tissue, testicular cell suspension, spermatogonial stem cell, gonadotoxicity, radiotherapy and chemotherapy.OUTCOMESThe feasibility of fertility restoration techniques using frozen-thawed TT and TCS has been proven in animal models. Efficient protocols for cryopreserving human TT exist and are currently applied in the clinic. For TCSs, the highest post-thaw viability reported after vitrification is 55.6 ± 23.8%. Yet, functional proof of fertility restoration in the human is lacking. In addition, few to no data are available on the safety aspects inherent to offspring generation with gametes derived from frozen-thawed TT or TCSs. Moreover, clarification is needed on whether it is better to cryopreserve TT or TCS.WIDER IMPLICATIONSFertility restoration techniques are very promising and expected to be implemented in the clinic in the near future. However, inter-center variability needs to be overcome and the gametes produced for reproduction purposes need to be subjected to safety studies. With the perspective of a future clinical application, there is a dire need to optimize and standardize cryopreservation and safety testing before using frozen-thawed TT of TCSs for fertility restoration.

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Role of serum on cryopreservation and subsequent viability of mouse bone marrow hemopoietic stem cells

Cited by 32 publications

References 8 publications

Effect of Antioxidants and Apoptosis Inhibitors on Cryopreservation of Murine Germ Cells Enriched for Spermatogonial Stem Cells

Effect of Antioxidants and Apoptosis Inhibitors on Cryopreservation of Murine Germ Cells Enriched for Spermatogonial Stem Cells

Successful liquid storage of peripheral blood stem cells at subzero non-freezing temperature

Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation

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