Role of serum tumor markers CA125 and CEA in non-small cell lung cancer

Salgia, Ravi; Harpole, David; Herndon, J.A; Pisick, Evan; Elias, Alexander; Skarin, Arthur T.

doi:10.1016/s0169-5002(00)80645-5

Cited by 40 publications

(56 citation statements)

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“…With regard to current biomarkers used in the clinic, this is the expected concentration range, giving us hope that new lung cancer markers should be detectable in serum. Through our method, we successfully identified proteins that are putative or currently used as biomarkers of lung cancer, including CEA (27,28), pro-GRP (29,31), SCC antigen (47,48), tumor M2-PK (49), NCAM (35)(36)(37), chromogranin A (29), and chromogranin B (30). In addition, we identified putative markers previously reported in lung-related proteomics studies such as member C1 of aldo-keto reductase family 1 identified by Huang et al (25) as dihydrodiol dehydrogenase and matrix metallopeptidase 1 found to be overexpressed in lung cancer patients and especially in late stage (18).…”

Section: Discussionmentioning

confidence: 99%

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Planque

Kulasingam

Smith

et al. 2009

Molecular & Cellular Proteomics

133

107

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Detection of lung cancer at an early stage is necessary for successful therapy and improved survival rates. We performed a bottom-up proteomics analysis using a twodimensional LC-MS/MS strategy on the conditioned media of four lung cancer cell lines of different histological backgrounds (non-small cell lung cancer: H23 (adenocarcinoma), H520 (squamous cell carcinoma), and H460 (large cell carcinoma); small cell lung cancer: H1688) to identify secreted or membrane-bound proteins that could be useful as novel lung cancer biomarkers. Proteomics analysis of the four conditioned media allowed identification of 1,830 different proteins (965, 871, 726, and 847 from H1688, H23, H460, and H520, respectively). All proteins were assigned a subcellular localization, and 38% were classified as extracellular or membrane-bound. We successfully identified the internal control proteins (also detected by ELISA), kallikrein-related peptidases 14 and 11, and IGFBP2. We also identified known or putative lung cancer tumor markers such as squamous cell carcinoma antigen, carcinoembryonic antigen, chromogranin A, creatine kinase BB, progastrin-releasing peptide, neural cell adhesion molecule, and tumor M2-PK. To select the most promising candidates for validation, we performed tissue specificity assays, functional classifications, literature searches for association to cancer, and a comparison of our proteome with the proteome of lung-related diseases and serum. Five novel lung cancer candidates, ADAM-17, osteoprotegerin, pentraxin 3, follistatin, and tumor necrosis factor receptor superfamily member 1A were preliminarily validated in the serum of patients with lung cancer and healthy controls. Our results demonstrate the utility of this cell culture proteomics approach to identify secreted and shed proteins that are potentially useful as serologi-

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Section: Discussionmentioning

confidence: 99%

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Planque

Kulasingam

Smith

et al. 2009

Molecular & Cellular Proteomics

133

107

View full text Add to dashboard Cite

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“…Some reports have indicated that CEA levels were significantly higher in patients with adenocarcinoma compared to patients with squamous cell carcinoma [31,34].…”

Section: Discussionmentioning

confidence: 98%

“…Salgia et al [31] stated that serum CEA levels were significantly lower in patients with early stage disease as compared to patients with unrespectable or metastatic disease. Nonaka et al [32] reported that serum CEA level reflected tumor size, but not tumor invasion.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value of CYFRA 21-1 and Carcinoembryonic Antigen in Non-Small Cell Lung Cancer

Said¹,

Abd-Elnaeem²,

Mohamed³

et al. 2015

J Clin Cell Immunol

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“…Several reports have indicated that S-CEA levels are significantly higher in patients with adenocarcinoma as compared to patients with squamous cell carcinoma (26)(27)(28)(29). Okada et al (26) reported that although S-CEA levels were significantly higher in patients with adenocarcinoma than in those with squamous cell carcinoma, but the proportion of CEApositive patients with adenocarcinoma (35.3%) was less than that of CEA-positive patients …”

Section: Discussionmentioning

confidence: 99%

Serum Carcinoembryonic Antigen Level as a Predictive Marker for Distant Metastasis in Non-Small Cell Lung Cancer

et al. 2007

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Aim: Elevated serum CEA (S-CEA) levels are sometimes attributable to the production of CEA by malignant cells, and in turn, the antigen itself can enhance the metastatic potential of malignant cells. We investigated the predictive role of S-CEA level for distant metastasis in nonsmall cell lung cancer (NSCLC).Methods: Consecutive 116 NSCLC patients referred to our department were retrospectively investigated. S-CEA levels was compared according to age, gender, smoking habits, histological type, distant metastases and clinical disease stage in all patients.Results: There were no significant relationship between S-CEA level and age, gender and smoking habits. There was significant difference in S-CEA level between M0 and M1 patients (p<0.001). S-CEA levels were not significantly different between symptomatic and silent metastases NSCLC patients (p=0.103). We could not find significant differences in S-CEA levels between T1-2 and T3-4 (p=0.141), N0-1 and N2-3 (p=0.672). In NSCLC, the area under the ROC curve was 0.728 (p<0.001), S-CEA threshold of 6.4 ng/mL has predictive sensitivity and specificity, for distant metastases as 69.8% and 73.0% respectively. In multivariate analysis including age, gender, smoking status, histologic type and S-CEA level that only S-CEA levels qualified as an independent predictive factor for distant metastases (p=0.001).Conclusion: We conclude that S-CEA levels were significant for predicting distant metastases. High levels of S-CEA may be an indication to perform routine investigation of distant metastases even in the absence of symptoms and signs.

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Role of serum tumor markers CA125 and CEA in non-small cell lung cancer

Cited by 40 publications

References 0 publications

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Prognostic Value of CYFRA 21-1 and Carcinoembryonic Antigen in Non-Small Cell Lung Cancer

Serum Carcinoembryonic Antigen Level as a Predictive Marker for Distant Metastasis in Non-Small Cell Lung Cancer

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