Role of several Staphylococcus aureus virulence factors on the inflammatory response in bovine mammary gland

Zecconi, A.; Cesaris, L.; Liandris, Emmanouil; Daprà, Valentina; Piccinini, R.

doi:10.1016/j.micpath.2006.01.001

Cited by 118 publications

(122 citation statements)

References 39 publications

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“…The aim of this study was to investigate the distribution of bacterial virulence determinants in the most prevalent S. aureus strain types causing lesions in rabbits that could possibly explain this host specificity. In the present study, the diversity of analysed virulence genes was high, as previously described in cows (Akineden et al, 2001;Zecconi et al, 2006) and humans (Peacock et al, 2002). Adhesins coa, spa, clfA, clfB, fib, sdrC and map/eap were positive in all the rabbit S. aureus isolates analysed in this study.…”

Section: Discussionsupporting

confidence: 84%

“…The rest of the adhesins showed variable prevalence: cna (94.2%), sdrD (89.9%), sdrE (78.3%), bbp (75.4%) and fnbB (59.4%). The high prevalence of cna was unexpected, as it was remarkably higher in this study than in isolates from humans (Nashev et al, 2004), other animals (Zecconi et al, 2006) or even rabbits (Vancraeynest et al, 2004). Most of the isolates were positive for 3 sdr adhesins (78%), which is the most common result described in the bibliography (Peacock et al, 2002), although some isolates are only positive to sdrC (7%) (Sabat et al, 2006).…”

Section: Discussionmentioning

confidence: 49%

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Screening of virulence genes in Staphylococcus aureus isolates from rabbits

Martín

Selva²,

Penadés³

et al. 2015

World Rabbit Sci.

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Staphylococcus aureus is a versatile pathogen able to cause disease in both humans and animals. In rabbits, this bacterium infects animals of different ages, producing several purulent lesions. The ability of S. aureus to cause disease depends on a combination of virulence factors. The aim of this study was therefore to investigate the distribution of bacterial virulence determinants in 69 S. aureus isolates from rabbits. Some virulence factors (7 adhesins, 1 toxin and 1 protease) were positive in all rabbit S. aureus isolates analysed, while others (1 adhesin and 10 toxins) were always negative. The remaining virulence factors were more variable among isolates. An association between genotype and the different profiles of virulence factors was observed, but not with the type of lesion (P<0.05). One strain of each genotype was further analysed by multilocus sequence typing, generating ST121, ST96 and ST2951, determining a greater number of enterotoxins in ST121 isolates compared to ST96 and ST2951 isolates, which could justify the different pathogenicity between strains.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 49%

Screening of virulence genes in Staphylococcus aureus isolates from rabbits

Martín

Selva²,

Penadés³

et al. 2015

World Rabbit Sci.

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“…Among the various pathogens isolated as causative agents of bovine mastitis, Staphylococcus aureus is considered to be a frequent cause affecting both the yield and quality of milk [1][2][3][4] . In numerous locations worldwide, mastitis has been found to respond poorly to antibiotic treatment 5,6 .…”

Section: Introductionmentioning

confidence: 99%

Untitled

Intrakamhaeng¹,

Komutarin²

2012

ScienceAsia

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Methicillin resistant Staphylococcus aureus (MRSA) strains are a global health concern in both human and veterinary medicine. In addition to β-lactam resistance, MRSA can carry resistance to other commonly used antibiotics. The present study sought to determine the antibiotic susceptibility profile and genetic relatedness of strains emerging in Thailand. A total of 229 isolates of S. aureus obtained from 598 mastitis cases were investigated for their susceptibility to several antibiotics. Among 229 isolates of S. aureus, 27 were found to be methicillin resistant and multidrug resistant. Multidrug resistant MRSA infection was detected in 4 provinces including Lopburi, Udon Thani, Khon Kaen and Saraburi. Multidrug resistant strains exhibited several antibiogram patterns (antibiotypes I to XII). The most frequent pattern was antibiotype VII, i.e., resistance to most commonly used antibiotics. Based on random amplification of polymorphic DNA (RAPD) technique and a combined amplicon-profile obtained with three primers, all isolates were grouped according to genetic similarity. Five different RAPD patterns (types I to V) were identified with 90% genetic similarity. The close genetic relatedness within RAPD type I was interesting because most RAPD type I isolates had antibiogram pattern III or VII. In addition, some isolates from different regions were identical in both antibiogram pattern and RAPD type. This revealed that some of the antibiotic-resistant isolates in our study were epidemic strains.

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“…SEs are known to function as potent superantigens that stimulate non-specific clonal T-cell proliferation, followed by a state of hyporesponsiveness to subsequent antigen stimulation and, thus, hinder the development of protective immunity, while promoting the persistence of bacteria in the host (11). SEs may also aggravate bovine intramammary infections throughout local cytokine release (40). SEs are produced by S. aureus after agr activation quorum-sensing system (QS), which consists of a sophisticated system to ensure that some bacteria functions only take place when a specific population density has been reached (3).…”

mentioning

confidence: 99%

Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity

Carlos¹,

Amaral²,

Vieira³

et al. 2010

Braz. J. Microbiol.

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Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains in the presence of 1.2 µg.mL -1 RGE, a significant inhibition of cell growth was observed using both spectrophotometry and ELISA assays. Twelve drugs were evaluated for their antimicrobial effects on culture RGE-treated cells using the disk diffusion method. Penicillin resistant strains became sensitive to the drug after RGE treatment. Furthermore, enterotoxin production by RGE-treated S. aureus was evaluated using a standardized ELISA method. Although staphylococcal LSA 88 bovine strain cells remained viable after exposure to the extract, enterotoxin production was precluded in 20% after RGE treatment. Significant interference in staphylococci cell density, drug sensitivity and enterotoxin secretion was observed after treatment. The study highlights the necessity to find new methods of disease prevention and new antibiotic therapies against staphylococcal infections.

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Role of several Staphylococcus aureus virulence factors on the inflammatory response in bovine mammary gland

Cited by 118 publications

References 39 publications

Screening of virulence genes in Staphylococcus aureus isolates from rabbits

Screening of virulence genes in Staphylococcus aureus isolates from rabbits

Untitled

Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity

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