Role of Silver Nanoparticles in Treatment of Plant Diseases

Sharma, Jyotsna; Singh, Vivek K.; Kumar, Anil; Shankarayan, Raju; Mallubhotla, Sharada

doi:10.1007/978-981-10-7140-9_20

Cited by 11 publications

(5 citation statements)

References 55 publications

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“…Chemical control is currently the most prevalent method of control, despite being hazardous to humans and the environment. In recent years, AgNPs have gradually been applied in the control of agricultural diseases due to their high efficiency and broad-spectrum antimicrobial activities, low resistance, and high safety ( Sharma et al, 2018 ; Malandrakis et al, 2019 ; Chen et al, 2020 ). This report firstly indicated the antifungal activity of AgNPs against four pathogens causing kiwifruit post-harvest rot from physiological, cytological, and transcriptomic levels.…”

Section: Discussionmentioning

confidence: 99%

The antifungal activity and mechanism of silver nanoparticles against four pathogens causing kiwifruit post-harvest rot

Pan

Deng

et al. 2022

Front. Microbiol.

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Post-harvest rot causes enormous economic loss to the global kiwifruit industry. Currently, there are no effective fungicides to combat the disease. It is unclear whether silver nanoparticles (AgNPs) are effective in controlling post-harvest rot and, if so, what the underlying antifungal mechanism is. Our results indicated that 75 ppm AgNPs effectively inhibited the mycelial growth and spore germination of four kiwifruit rot pathogens: Alternaria alternata, Pestalotiopsis microspora, Diaporthe actinidiae, and Botryosphaeria dothidea. Additionally, AgNPs increased the permeability of mycelium’s cell membrane, indicating the leakage of intracellular substance. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations revealed that AgNPs induced pathogen hypha shrinkage and distortion, as well as vacuolation in hypha cells, implying that AgNPs caused cellular and organelle structural degradation. The transcriptome sequencing of mycelium treated with AgNPs (24 h / 48 h) was performed on the Illumina Hiseq 4000 sequencing (RNA-Seq) platform. For the time points of 24 h and 48 h, AgNPs treatment resulted in 1,178 and 1,461 differentially expressed genes (DEGs) of A. alternata, 517 and 91 DEGs of P. microspora, 1,287 and 65 DEGs of D. actinidiae, 239 and 55 DEGs of B. dothidea, respectively. The DEGs were found to be involved in “catalytic activity,” “small molecule binding,” “metal ion binding,” “transporter activity,” “cellular component organization,” “protein metabolic process,” “carbohydrate metabolic process,” and “establishment of localization.” Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis also revealed that “carbohydrate metabolism,” “amino acid metabolism,” “energy metabolism,” and “xenobiotics biodegradation and metabolism” of “metabolism processes” were the most highly enriched pathways for these DEGs in four pathogens, with “cellular processes” being particularly enriched for B. dothidea. Furthermore, quantitative polymerase chain reactions (qPCRs) were used to validate the RNA-seq results. It was also confirmed that AgNPs could significantly reduce the symptoms of kiwifruit rot without leaving any Ag+ residue on the peel and flesh of kiwifruit. Our findings contributed to a better understanding of the antifungal effect and molecular mechanisms of AgNPs against pathogens causing kiwifruit post-harvest rot, as well as a new perspective on the application of this novel antifungal alternative to fruit disease control.

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Section: Discussionmentioning

confidence: 99%

The antifungal activity and mechanism of silver nanoparticles against four pathogens causing kiwifruit post-harvest rot

Pan

Deng

et al. 2022

Front. Microbiol.

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“…They offer controlled release and can stabilize active ingredients without the need for organic solvents. However, they have limitations, including low loading efficiency and potential leakage during storage (Sharma et al 2018).…”

Section: Solid Lipid Nanoparticles (Slns)mentioning

confidence: 99%

POTENTIAL ROLE OF SILVER NANOPARTICLES (AgNPs) AND ZINC NANOPARTICLES (ZnNPs) FOR PLANT DISEASE MANAGEMENT

2023

Agrobiol. Rec.

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Plant diseases pose a significant threat to global agriculture, impacting crop yield and food security. Traditional methods of disease management often involve the application of chemical pesticides, which raise environmental and health concerns. In recent years, nanotechnology has emerged as a promising avenue for sustainable and effective plant disease management. This review provides an overview of the applications and mechanisms of action of silver nanoparticles (AgNPs) and zinc nanoparticles (ZnNPs) in combating plant pathogens. AgNPs and ZnNPs have garnered attention due to their antimicrobial properties, biocompatibility, and versatility. They exhibit multifaceted mechanisms of action, including membrane disruption, generation of reactive oxygen species (ROS), interference with enzymatic processes, and inhibition of microbial growth. These nanoparticles can effectively target a wide range of plant pathogens, including bacteria, fungi, and viruses. Moreover, they can be used as carriers for the controlled release of antimicrobial agents and nutrients, further enhancing their utility in agriculture. Furthermore, AgNPs and ZnNPs have demonstrated compatibility with environmentally friendly and sustainable agricultural practices. Their targeted delivery reduces chemical waste and minimizes environmental contamination. Additionally, these nanoparticles can stimulate the plant's innate defense mechanisms, enhancing its resilience against diseases. This review highlights the potential of AgNPs and ZnNPs as innovative tools for plant disease management, offering sustainable and effective alternatives to conventional pesticides. The ongoing research in this field aims to optimize nanoparticle formulations, assess their safety, and develop integrated disease management strategies to enhance agricultural sustainability and food production.

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“…Due to the low degradability of nanoparticles in the natural environment [49], the full development of this sector still requires in-depth studies on the impacts of the different active ingredients used as nanoparticles to determine the long-term effects of nanoparticles on the environment and their residual content in food. The field applications of silver nanoparticles retained as effective antimicrobials are the most striking example of this cautious approach [124]. The need to formulate precise guidelines for nanoparticle utilisation in the field is also required [114,125].…”

Section: Nanotechnologymentioning

confidence: 99%

Sustainable Management of Diseases in Horticulture: Conventional and New Options

Scortichini¹

2022

Horticulturae

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To reduce the impact of chemical pesticides on the environment, there are relevant efforts to enhance the possibility of controlling plant diseases using environmentally friendly biocontrol agents or natural products that show pathogen control capacity. The European Union, FAO, and the United Nations largely promote and finance projects and programs in order to introduce crop protection principles that can attain sustainable agriculture. Preventive measures related to the choice of cultivars, soil fertility, integrated pest management (IPM), and organic farming strategies are still the basis for obtaining satisfactory crop yields and reducing classical pesticide utilisation through the application of commercially available and ecofriendly control agents. Effective pathogen detection at borders to avoid quarantine pathogens is mandatory to reduce the risk of future epidemics. New technical support for the development of sustainable pathogen control is currently being provided by forecasting models, precision farming, nanotechnology, and endotherapy. New biocontrol agents and natural products, disease management through plant nutrition, systemic resistance inducers, and gene-silencing technology will provide solutions for obtaining satisfactory disease control in horticulture. The “multi-stakeholder partnership” strategy can promote the implementation of sustainable crop protection.

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Role of Silver Nanoparticles in Treatment of Plant Diseases

Cited by 11 publications

References 55 publications

The antifungal activity and mechanism of silver nanoparticles against four pathogens causing kiwifruit post-harvest rot

The antifungal activity and mechanism of silver nanoparticles against four pathogens causing kiwifruit post-harvest rot

POTENTIAL ROLE OF SILVER NANOPARTICLES (AgNPs) AND ZINC NANOPARTICLES (ZnNPs) FOR PLANT DISEASE MANAGEMENT

Sustainable Management of Diseases in Horticulture: Conventional and New Options

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