Role of SKD1 Regulators LIP5 and IST1-LIKE1 in Endosomal Sorting and Plant Development

Buono, Rafael Andrade; Páez-Valencia, Julio; Miller, Nathan D.; Goodman, Kaija; Spitzer, Christoph; Spalding, Edgar P.; Otegui, Marisa S.

doi:10.1104/pp.16.00240

Cited by 62 publications

(56 citation statements)

References 104 publications

(150 reference statements)

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“…7F). Accumulation of PM proteins in the tonoplast usually results from a failure to sort proteins targeted for vacuolar degradation into ILVs, as in the case of ESCRT mutants (Spitzer et al, 2009;Buono et al, 2016). Therefore, to investigate the potential role of VPS38 in the degradation of endocytosed membrane proteins, we incubated PIN2-GFP-and BRI1-GFP-expressing seedlings in the dark and in the presence of ConA, both of which were used to monitor GFP fluorescence in the vacuolar lumen (Kleine-Vehn et al, 2008).…”

Section: Vps38 Is Crucial For Efficient Vacuolar Degradation Of Endocmentioning

confidence: 99%

Vacuolar Trafficking Protein VPS38 Is Dispensable for Autophagy

et al. 2017

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Phosphatidylinositol 3-P (PI3P) is a signaling molecule that controls a variety of processes in endosomal, autophagic, and vacuolar/lysosomal trafficking in yeasts and mammals. Vacuolar protein sorting 34 (Vps34) is a conserved PI3K present in multiple complexes with specific functions and regulation. In yeast, the PI3K complex II consists of Vps34p, Vps15p, Vps30p/ Atg6p, and Vps38p, and is essential for vacuolar protein sorting. Here, we describe the Arabidopsis (Arabidopsis thaliana) homolog of yeast Vps38p and human UV radiation resistance-associated gene protein. Arabidopsis VPS38 interacts with VPS30/ATG6 both in yeast and in planta. Although the level of PI3P in Arabidopsis vps38 mutants is similar to that in wild type, vps38 cells contain enlarged multivesicular endosomes and fewer organelles enriched in PI3P than the wild type. The vps38 mutants are defective in the trafficking of vacuolar cargo and its receptor VACUOLAR SORTING RECEPTOR2;1. The mutants also exhibit abnormal cytoplasmic distributions of endocytic cargo, such as auxin efflux carriers PINFORMED1 (PIN1) and PIN2. Constitutive autophagy is normal in the mutants but starvation-induced autophagy was slightly inhibited. We conclude that Arabidopsis VPS38 is dispensable for autophagy but essential for efficient targeting of biosynthetic and endocytic cargo to the vacuole.

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Section: Vps38 Is Crucial For Efficient Vacuolar Degradation Of Endocmentioning

confidence: 99%

Vacuolar Trafficking Protein VPS38 Is Dispensable for Autophagy

et al. 2017

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“…SERK2 is Leu-rich repeat receptor-like kinase that has been shown to be involved in brassinosteroid signaling (Gou et al, 2012). The identified loci also included regulators of ABA signaling (SOAR1; Mei et al, 2014) and auxin biosynthesis (YUC4; Cheng et al, 2006) as well as genes engaged with sugar signaling (TPPC; Schluepmann et al, 2004), cell division (CYCA2;2; Vandepoele et al, 2002), microtubule functioning (At2g188876), mRNA metabolism (At5g28220 and At5g11412), and endosomal sorting (ISTL1; Buono et al, 2016).…”

Section: Identification Of the Genetic Components Of Responses To Doumentioning

confidence: 99%

Phosphate-dependent root system architecture responses to salt stress

et al. 2016

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Nutrient availability and salinity of the soil affect the growth and development of plant roots. Here, we describe how inorganic phosphate (Pi) availability affects the root system architecture (RSA) of Arabidopsis (Arabidopsis thaliana) and how Pi levels modulate responses of the root to salt stress. Pi starvation reduced main root length and increased the number of lateral roots of Arabidopsis Columbia-0 seedlings. In combination with salt, low Pi dampened the inhibiting effect of mild salt stress (75 mM) on all measured RSA components. At higher salt concentrations, the Pi deprivation response prevailed over the salt stress only for lateral root elongation. The Pi deprivation response of lateral roots appeared to be oppositely affected by abscisic acid signaling compared with the salt stress response. Natural variation in the response to the combination treatment of salt and Pi starvation within 330 Arabidopsis accessions could be grouped into four response patterns. When exposed to double stress, in general, lateral roots prioritized responses to salt, while the effect on main root traits was additive. Interestingly, these patterns were not identical for all accessions studied, and multiple strategies to integrate the signals from Pi deprivation and salinity were identified. By genome-wide association mapping, 12 genomic loci were identified as putative factors integrating responses to salt stress and Pi starvation. From our experiments, we conclude that Pi starvation interferes with salt responses mainly at the level of lateral roots and that large natural variation exists in the available genetic repertoire of accessions to handle the combination of stresses.

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“…The peak SNP S4_30201599 resides within a microRNA locus (zma-MIR2275a) and is located 1,146 bp from a gene ( Zm00001d049479 ) encoding an INCREASED SALT TOLERANCE1-LIKE1 (ISTL1) protein (Figure 2; Table S8). ISTL1 is the most probable candidate gene underlying this association signal because it is predicted to function together with the endosomal sorting complex required for transport machinery (ESCRT) and ATPase VACUOLAR PROTEIN SORTING4 (VPS4) in the formation of multivesicular bodies (MVBs) (Hill and Babst 2012; Buono et al . 2016).…”

Section: Resultsmentioning

confidence: 99%

“…Another membrane-trafficking-related candidate encodes an IST1 family protein closely related to Arabidopsis ISTL1 (Buono et al . 2016).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study for maize leaf cuticular conductance identifies candidate genes involved in the regulation of cuticle development

Gore

Smith

Meng

et al. 2019

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The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study of gc of adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA in 2016 and 2017). Five genomic regions significantly associated with gc were resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction of gc in locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control of gc and have the potential to help breeders more effectively develop drought-tolerant maize for target environments. Article summary The cuticle serves as the major barrier to water loss when stomata are closed at night and under water-limited conditions and potentially relevant to drought tolerance in crops. We performed a genome-wide association study to elucidate the genetic architecture of natural variation for maize leaf cuticular conductance. We identified epidermally expressed candidate genes that are potentially involved in cuticle biosynthesis, trafficking and deposition, cutin polymerization, and cell wall modification. Finally, we observed moderately high predictive abilities for whole-genome prediction of leaf cuticular conductance. Collectively, these findings may help breeders more effectively develop drought-tolerant maize.

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Role of SKD1 Regulators LIP5 and IST1-LIKE1 in Endosomal Sorting and Plant Development

Cited by 62 publications

References 104 publications

Vacuolar Trafficking Protein VPS38 Is Dispensable for Autophagy

Vacuolar Trafficking Protein VPS38 Is Dispensable for Autophagy

Phosphate-dependent root system architecture responses to salt stress

Genome-wide association study for maize leaf cuticular conductance identifies candidate genes involved in the regulation of cuticle development

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