Role of some fermentation parameters on cyclosporin A production by a new isolate of Aspergillus terreus

Abstract

A local isolate of Aspergillus terreus was selected among different microorganisms as a new cyclosporin A (Cy A) producing culture. The formation of Cy A was investigated under different fermentation conditions (including selection of the cultivation medium, fermentation time course, inoculum nature, medium volume, agitation rate, pH value). Relatively high Cy A productivities were maintained when the fermentation process was carried out using a medium composed of (g/L): glucose, 50; bactopeptone, 10; KH(2)PO(4), 5; KCl, 2.5; pH 5.3, inoculated with 2% standard inoculum of 48 h age, shaken at 200 rpm for 10 days.

Some optimal culture conditions for production of cyclosporin a by Fusarium roseum

“…However we found two papers reported the extraction of Cyc A from the culture filtrates and mycelia of both Tolypocladium species (4) and Aspergillus terreus (28). Our results showed that high concentration of the synthesized Cyc A by F. roseum was produced in the fungal culture filtrate and a lower level was produced in the fungal mycelia.…”

“…Some reports used the incubation temperature at 27 ºC for production of Cyc A (18,28,38). The maximum Cyc A production has been reported to vary with respect to the fermentation time course, where Sakamoto et al (27) found that the best time span for Cyc A production was 10 days, Isaac et al (18) found that 12 days of growth is a good period for Cyc A production from T. inflatum UAMH 2472.…”

“…Tolypocladium inflatum (14), Fusarium solani (29) Neocosmospora varinfecta (24) and Aspergillus terreus (28). In this paper, we briefly describe the optimum fermentation conditions and medium requirements necessary for Cyc A production by Fusarium roseum, a microorganism that has not yet been studied for this target.…”

Studies on the application of immobilization technique for the production of cyclosporin A by a local strain of Aspergillus terreus

“…The yield of immobilized cell culture was up to three-fold higher than for the free cells system. In addition, Lee and Agathos (1991) and Traber (1998) compared the physiological and environmental effects of Cy A production by suspended and immobilized cells of T. inflatum, and found that there was a significant difference in precursor flow between the immobilized and free cell systems particularly in the presence of L-valine. In a previous communication (Sallam et al, 2003), we selected a local isolate of A. terreus as a new Cy A producing fungus. The fermentation of Cy A was investigated under different fermentation and nutritional conditions.…”

Modification of c and n sources for enhanced production of cyclosporin 'a' by Aspergillus Terreus

“…Other soil inhabiting filamentous fungi like Fusarium solani (26) Neocosmospora vasinfecta (24) and Fusarium oxysporum (13) have also been reported to produce lower levels of cyclosporins. Recently, different strains of A. terreus have been identified as new producers of cyclosporine 'A' (27,28).…”