Role of Sonography for Implantation and Sequential Evaluation of a VX2 Rabbit Liver Tumor Model

Luo, Wen; Zhou, Xiaodong; Zheng, Xiaoying; He, Guangbin; Yu, Ming; Li, Qiuyang; Liu, Qing

doi:10.7863/jum.2010.29.1.51

Cited by 15 publications

(15 citation statements)

References 27 publications

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“…12 young NZW rabbits (each 3.0 to 3.5 kg) had Vx2 tumors surgically implanted (1.0 – 1.5 mm 3 ) in an anterior segment of their liver under sterile conditions via a small laparotomy incision according to well developed protocols (Gannon, Cherukuri et al 2007; Luo, Zhou et al 2009). Two weeks later, an intrahepatic VX2 tumor 1.0–1.5 cm in diameter was present in all animals.…”

Section: Methodsmentioning

confidence: 99%

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Biodistribution and acute toxicity of naked gold nanoparticles in a rabbit hepatic tumor model

Glazer¹,

Zhu²,

Hamir

et al. 2010

Nanotoxicology

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There is a paucity of data regarding the safety of administering solid gold nanoparticles (AuNPs) in large animal tumor models. We assessed the acute toxicity and biodistribution of 5 nm and 25 nm solid AuNPs in New Zealand White rabbits (n = 6 in each) with implanted liver Vx2 tumors 24 hours after intravenous injection. Gold concentration was determined by inductively coupled plasma atomic emission spectrometry (ICP) and imaged with transmission electron microscopy (TEM). There was no clinico-pathologic evidence of renal, hepatic, pulmonary, or other organ dysfunction. After 25 nm AuNP administration, the concentration of white blood cells increased after treatment (p = 0.001). Most other blood studies were unchanged. AuNPs were distributed to the spleen, liver, and Vx2 tumors, but not to other tissues. The urinary excretion of AuNPs was bimodal as measured by ICP. 25 nm AuNPs were more evenly distributed throughout tissues and may be better tools for medical therapy.

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Section: Methodsmentioning

confidence: 99%

“…Briefly, Vx2 tumors are very aggressive with nearly 100% uptake. Nearly all tumors are friable and hypervascular with frank central necrosis by 15–20 days after implantation (Luo, Zhou et al 2009). In our experience, most animals are euthanized for symptomatic pulmonary metastases within 30 to 45 days after implantation.…”

Section: Methodsmentioning

confidence: 99%

Biodistribution and acute toxicity of naked gold nanoparticles in a rabbit hepatic tumor model

Glazer¹,

Zhu²,

Hamir

et al. 2010

Nanotoxicology

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“…Namely, the particles were used to enhance US imaging contrast, as well as to enable US-mediated delivery of targeted gene and drug. For this demonstration, we established a rabbit renal tumor model by implanting VX2 tumor cells on the rabbit renal parenchyma (see Methods) 38. The implanted tumor expressed HER2 as measured by immunohistochemistry and Western blotting (Supplementary Material: Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultrasound-Mediated Gene and Drug Delivery Using a Microbubble-Liposome Particle System

Yoon¹,

Kwon²,

Cho³

et al. 2014

Theranostics

102

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Theranostic agents present a promising clinical approach for cancer detection and treatment. We herein introduce a microbubble and liposome complex (MB-Lipo) developed for ultrasound (US) imaging and activation. The MB-Lipo particles have a hybrid structure consisting of a MB complexed with multiple Lipos. The MB components are used to generate high echo signals in US imaging, while the Lipos serve as a versatile carrier of therapeutic materials. MB-Lipo allows high contrast US imaging of tumor sites. More importantly, the application of high acoustic pressure bursts MBs, which releases therapeutic Lipos and further enhances their intracellular delivery through sonoporation effect. Both imaging and drug release could thus be achieved by a single US modality, enabling in situ treatment guided by real-time imaging. The MB-Lipo system was applied to specifically deliver anti-cancer drug and genes to tumor cells, which showed enhanced therapeutic effect. We also demonstrate the clinical potential of MB-Lipo by imaging and treating tumor in vivo.

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“…The degree to which VX2 tumors mimic the morphology and behaviour of pancreatic tumors found in humans is unknown and since the VX2 cell line is a naturally occurring rabbit squamous cell carcinoma derived from virus-induced papilloma (33), it cannot be assumed to have any structural or molecular similarity to human pancreatic cancer. In fact, previous work by our team has found VX2 tumors implanted into the liver and thigh to have a soft, diffuse consistency, which is in contrast to the classical description of human ductal adenocarcinomas of the pancreas as firm, fibrous and marble-like in nature (34–36).…”

Section: Discussionmentioning

confidence: 99%

Perfusion CT Estimates Photosensitizer Uptake and Biodistribution in a Rabbit Orthotopic Pancreatic Cancer Model

et al. 2015

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Objectives It was hypothesized that perfusion computed tomography (CT) blood flow (BF), blood volume (BV) and vascular permeability surface-area (PS) product parameters would be predictive of therapeutic anti-cancer agent uptake in pancreatic cancer, facilitating image-guided interpretation of human treatments. The hypothesis was tested in an orthotopic rabbit model of pancreatic cancer, by establishing the model, imaging with endoscopic ultrasound and contrast CT, and spatially comparing the perfusion maps to the ex vivo uptake values of injected photosensitizer, vertepofin. Materials and Methods Nine New Zealand White rabbits underwent direct pancreas implantation of VX2 tumors and CT perfusion or endoscopic ultrasound was performed 10 days post-implantation. Verteporfin was injected during CT imaging and tissue was removed 1 h post-injection for frozen tissue fluorescence scanning. Region-of-interest comparisons of CT data with ex vivo fluorescence and histopathological staining were performed. Results DCE-CT showed enhanced BF, BV, and PS in the tumor rim, and decreased BF, BV and PS in the tumor core. Significant correlations were found between ex vivo verteporfin concentration and each of BF, BV, and PS. Conclusions The efficacy of verteporfin delivery in tumors is estimated by perfusion CT, providing a non-invasive method of mapping photosensitizer dose.

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Role of Sonography for Implantation and Sequential Evaluation of a VX2 Rabbit Liver Tumor Model

Abstract: Sonographically guided implantation achieved a good success rate with convenient inoculation performance. Conventional gray scale, CD, CE PIH, and CE CD sonography were useful in sequential evaluation of tumor growth and characteristic vascularity.

Cited by 15 publications

References 27 publications

Biodistribution and acute toxicity of naked gold nanoparticles in a rabbit hepatic tumor model

Biodistribution and acute toxicity of naked gold nanoparticles in a rabbit hepatic tumor model

Ultrasound-Mediated Gene and Drug Delivery Using a Microbubble-Liposome Particle System

Perfusion CT Estimates Photosensitizer Uptake and Biodistribution in a Rabbit Orthotopic Pancreatic Cancer Model

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