Role of specific immunoglobulin E in diagnosis of acute toxoplasma infection and toxoplasmosis

Wong, Serre-Yu; Hajdú, Melinda; Ramirez, Raymund; Thulliez, P.; McLeod, Rima; Remington, Jack S.

doi:10.1128/jcm.31.11.2952-2959.1993

Cited by 90 publications

(47 citation statements)

References 16 publications

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“…IgA known for its role in neutralization of toxins and pathogenic microbes, (29,30), while IgM had been associated with neutrophils phagocytic activity and complement cascade activation, which might result in killing of the T. gondii parasite (31,32). In this study, no significant change in IgE was observed after vaccination with TgMDH, although previous investigations showed the existence of this antibody during diagnosis of T. gondii infection (33,34).…”

Section: Discussioncontrasting

confidence: 66%

Immunological response and protection of mice immunized with plasmid encoding Toxoplasma gondii glycolytic enzyme malate dehydrogenase

Hassan

Wang

et al. 2014

Parasite Immunology

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Toxoplasma gondii Malate dehydrogenase (TgMDH) plays an important role as part of the energy production cycle. In this investigation, immunological changes and protection efficiency of this protein delivered as a DNA vaccine have been evaluated. Mice were intramuscularly immunized with pTgMDH, followed by challenge with virulent T. gondii RH strain, 2 weeks after the booster immunization. Compared to the control groups, the results showed that pTgMDH has stimulated specific humoral response as demonstrated by significant high titers of total IgG and subclasses IgG1 and IgG2a , beside IgA and IgM, but not IgE. Analysis of cytokine profiles revealed significant increases of IFN-γ, IL-4 and IL-17, while no significant changes were detected in TGF-β1. In cell-mediated response, both T lymphocytes subpopulations CD4(+) and CD8(+) were positively recruited as significant percentages were recorded in response to immunization with TgMDH. Significant long survival rate, 17 days, has been observed in the TgMDH vaccinated group, in contrast with control groups which died within 8-9 days after challenge. These results demonstrated that TgMDH could induce significant immunological responses leading to a considerable level of protection against acute toxoplasmosis infection.

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Section: Discussioncontrasting

confidence: 66%

Immunological response and protection of mice immunized with plasmid encoding Toxoplasma gondii glycolytic enzyme malate dehydrogenase

Hassan

Wang

et al. 2014

Parasite Immunology

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“…IgE ELISA shows lower sensitivity than IgM assays [29][30][31], but the present study shows that, as with IgA, decreased sensitivity is not necessarily caused by failure to form these antibodies, and can also be caused by delayed or early cessation of antibody production, resulting in IgE antibodies being undetected during sample collection. Of all the antibody classes, IgE has the shortest duration, with positive results for up to 6 months following onset of symptoms, but this period can vary from 3 -5 months to 11 months [31][32][33][34]. Foudrinier et al [30] described two different kinetic patterns in asymptomatic seroconverters (short duration) and in seroconverters with overt toxoplasmosis, in which high IgE levels persist for a number of months.…”

contrasting

confidence: 57%

Evaluation of a commercial IgE ELISA in comparison with IgA and IgM ELISAs, IgG avidity assay and complement fixation for the diagnosis of acute toxoplasmosis

Kodym

Machala

Roháčová

et al. 2007

Clinical Microbiology and Infection

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A panel of sera from patients with known case histories representative of acute toxoplasmosis (primarily lymphadenopathy, n = 106), latent toxoplasmosis (asymptomatic, n = 368) and negative samples (n = 54) was used to evaluate the capacity of five serological tests to differentiate among patients with acute or latent toxoplasmosis and non-infected individuals. Positive IgA, IgE and IgM ELISA results and low IgG avidity and complement fixation test (CFT) titres of >or=256 were considered to be indicative of acute toxoplasmosis. The most sensitive methods were IgM ELISA (98.1%) and CFT (97.1%), albeit with low specificity (65.0% and 64.5%, respectively) and positive predictive values (43.3% and 42.7%, respectively). IgG avidity assay and IgE ELISA had the highest specificity (97.7% and 91.7%, respectively) and the highest positive predictive values (89.4% and 75.6%, respectively). The best association between serological results and clinical findings was obtained with IgE ELISA (86%, as expressed via Youden's index). In a subset of 259 samples categorised by the period between the onset of clinical symptoms and sampling, >50% of patients had enlarged lymph nodes for <4 months, despite a broad range of differences. However, IgM remained positive for 12-18 months, IgA for 6-9 months and IgE for 4-6 months. IgG avidity remained low for a maximum of 4 months, after which avidity increased despite the persistence of enlarged lymph nodes and a positive IgE assay. Detection of IgE appears to be a highly specific test for confirming the acute nature of Toxoplasma infections that have been detected by other sensitive methods.

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“…PAMF serological tests. The Sabin-Feldman dye test (14), direct agglutination test (6), double-sandwich IgM ELISA (10), IgA ELISA (15), IgE ISAGA (18), IgE ELISA (18), and AC/HS (4) were performed as previously described. The Sabin-Feldman dye test is considered the "gold standard" for detection of IgG antibodies against T. gondii.…”

Section: Methodsmentioning

confidence: 99%

“…Comparisons were performed both retrospectively on selected sera that had previously been tested in our laboratory and prospectively on consecutive sera from adults that had been received by our laboratory for diagnostic testing. In order to investigate the significance of detection of IgM antibodies by either method, results obtained in additional serological tests, including IgA (15) and IgE ELISAs (18), IgE immunosorbent agglutination assay (ISAGA) (18), and differential agglutination (AC/HS) tests (4), as well as results of follow-up serological tests, were analyzed.…”

mentioning

confidence: 99%

Study of Abbott Toxo IMx system for detection of immunoglobulin G and immunoglobulin M toxoplasma antibodies: value of confirmatory testing for diagnosis of acute toxoplasmosis

et al. 1996

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We compared the Abbott Toxo immunoglobulin G (IgG) and IgM IMx assays with the Sabin-Feldman dye test and an IgM enzyme-linked immunosorbent assay (ELISA) in 398 serum samples previously tested in our laboratory (retrospective group) and 1,000 consecutive serum samples, tested as they were received in our laboratory in 1995 (prospective group). In the retrospective group, the IgG IMx had a sensitivity of 100%, specificity of 99.0%, positive predictive value of 99.0%, negative predictive value of 100%, and overall agreement of 99.5%. The percentages for the IgM IMx were 97.8, 99.0, 98.9, 98, and 98.4%, respectively. In the prospective group, the IgG IMx had a sensitivity of 97.8%, specificity of 98.7%, positive predictive value of 97.8%, negative predictive value of 98.7%, and overall agreement of 98.4%. The percentages for the IgM IMx were 88.3, 95.9, 74.7, 98.3, and 95%. A serological profile (IgA and IgE antibodies and the differential agglutination [AC/HS] test) was performed in an attempt to resolve discrepancies. Of 21 serum samples that were positive in the IgM IMx and negative in the IgM ELISA, 19 had serological profiles which were most compatible with an infection acquired in the distant past. Of 8 serum samples which were positive in the IgM ELISA and negative in the IgM IMx, 5 had serological profiles which were most compatible with an infection acquired in the distant past. Of the 55 serum samples that were positive in both IgM tests, 21 were from patients who had serological profiles which were most compatible with an infection acquired in the distant past. In conclusion, our data highlight the importance of confirmatory testing for the diagnosis of recently acquired infection with Toxoplasma gondii. When compared with the dye test and IgM ELISA, the Toxo IgG and IgM IMx assays, respectively, revealed high overall agreement in the retrospective and prospective study.

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Role of specific immunoglobulin E in diagnosis of acute toxoplasma infection and toxoplasmosis

Cited by 90 publications

References 16 publications

Immunological response and protection of mice immunized with plasmid encoding Toxoplasma gondii glycolytic enzyme malate dehydrogenase

Immunological response and protection of mice immunized with plasmid encoding Toxoplasma gondii glycolytic enzyme malate dehydrogenase

Evaluation of a commercial IgE ELISA in comparison with IgA and IgM ELISAs, IgG avidity assay and complement fixation for the diagnosis of acute toxoplasmosis

Study of Abbott Toxo IMx system for detection of immunoglobulin G and immunoglobulin M toxoplasma antibodies: value of confirmatory testing for diagnosis of acute toxoplasmosis

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