Role of SPI-1 in the interactions of Salmonella Typhimurium with porcine macrophages

Boyen, Filip; Pasmans, Frank; Donné, Eef; Immerseel, Filip Van; Adriaensen, Connie; Hernálsteens, Jean-Pierre; Ducatelle, Richard; Haesebrouck, Freddy

doi:10.1016/j.vetmic.2005.10.018

Cited by 29 publications

(36 citation statements)

References 28 publications

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“…As interleukin-8 (IL-8) is a major cytokine in the development of Salmonella-induced intestinal inflammation, 23 one could try to assess the ability of a Salmonella strain to induce an intestinal inflammation response by measuring the IL-8 production using either macrophages or epithelial cells. 12,23 However, as other cytokines also play a role in the attraction of neutrophils to the lumen of the gut, 23 and also because enteritis is the result of an extensive interplay of different receptors on different cell types, 23 the intestinal loop model is a much more physiological and reliable model. Intracellular survival and proliferation inside macrophages have been shown to contribute to persistent infections of Salmonella in mice.…”

Section: Discussionmentioning

confidence: 99%

Porcinein vitroandin vivomodels to assess the virulence ofSalmonella entericaserovar Typhimurium for pigs

et al. 2009

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Salmonella Typhimurium infections in pigs pose an important human health hazard. One promising control measure is the development of live attenuated vaccine strains using defined knockout mutants. Preferably, screening of candidate knockout vaccine strains for attenuation should first be done in models allowing testing of a large number of strains. Thereafter, a limited number of selected strains should be further characterized in an experimental infection model in pigs. The aim of the present study was to develop such models. The invasive and proliferative characteristics of S. Typhimurium were assessed in both a non-polarized and a polarized porcine intestinal epithelial cell line. Neutrophils obtained from porcine blood were used to study the capacity of Salmonella to withstand killing by these phagocytes. The ability to induce an intestinal inflammatory response was investigated in a terminal intestinal loop model. The systemic phase of infection was mimicked by studying the uptake and intracellular survival of S. Typhimurium in porcine pulmonary alveolar macrophages and peripheral blood monocytes. These models should allow screening for attenuated strains. For further characterization, an experimental infection model was established, providing extensive data on the course of an oral infection and the optimal time points for colonization (day 5 postinoculation [ pi]) and persistency (days 21-28 pi) in pigs. In conclusion, screening for virulence of S. Typhimurium strains with subsequent confirmation for a subset of strains in a well-defined experimental infection model would significantly reduce the number of experimental pigs required.

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Section: Discussionmentioning

confidence: 99%

Porcinein vitroandin vivomodels to assess the virulence ofSalmonella entericaserovar Typhimurium for pigs

et al. 2009

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“…Salmonella Typhimurium strain 112910a, phage type 120/ ad, was isolated from a pig stool sample and has been shown to persist in tonsils, intestines and gut-associated lymph nodes of experimentally infected pigs for at least 28 days (unpublished data). The construction and characterization of mutants with non-polar deletion mutations in the genes encoding the major SPI-1 regulatory protein HilA, the SPI-1 translocator/effector protein SipB and the SPI-1 effector protein SipA have been described before [14]. An invasive, spontaneous nalidixic acid resistant derivative of the wild type strain (WT nal ) was used for the mixed infection assay in order to minimise irrelevant bacterial growth when plating out intestinal and faecal samples.…”

Section: Methodsmentioning

confidence: 99%

Salmonella Typhimurium SPI-1 genes promote intestinal but not tonsillar colonization in pigs

Boyen

Pasmans

Immerseel

et al. 2006

Microbes and Infection

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“…To obtain highly invasive late logarithmic cultures for invasion assays [23], 2 lL of a stationary phase culture was inoculated in 5 mL LB and grown for 5 h at 37°C without aeration. The construction and characterization of a deletion mutant in the gene encoding the SPI-1 translocator/effector protein SipB has been described before [5]. Salmonella Typhimurium strains carrying the plasmid containing either the hilA-luxCDABE or the ssrA-luxCDABE transcriptional fusions were used in the virulence gene expression experiments [5].…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

“…The construction and characterization of a deletion mutant in the gene encoding the SPI-1 translocator/effector protein SipB has been described before [5]. Salmonella Typhimurium strains carrying the plasmid containing either the hilA-luxCDABE or the ssrA-luxCDABE transcriptional fusions were used in the virulence gene expression experiments [5]. For fluorescence microscopy, the pFPV25.1 plasmid expressing green fluorescent protein under the constitutive promoter of rpsM was used [6,35,36].…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

The mycotoxin deoxynivalenol promotes uptake ofSalmonellaTyphimurium in porcine macrophages, associated with ERK1/2 induced cytoskeleton reorganization

Vandenbroucke¹,

Croubels²,

Verbrugghe³

et al. 2009

Vet. Res.

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-Both the mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium are major issues in swine production. This study aimed at examining the interaction between DON and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcine macrophages. Incubation with 0.25 lg/mL of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of Salmonella Typhimurium was determined. At low non-cytotoxic concentrations, as can be found in the serum of pigs, DON did not have any effect on either growth or virulence gene expression of Salmonella Typhimurium. However, when the invasion and intracellular survival of Salmonella Typhimurium in macrophages preexposed to 0.025 lg/mL of DON was examined, DON significantly promoted the uptake of Salmonella Typhimurium into macrophages. The enhanced uptake coincided with marked F-actin reorganization of the cells, which was due to the activation of extracellular signal-regulated protein kinase 1/2 (ERK1/2). These results suggest that low but relevant concentrations of DON modulate the innate immune system and could thus increase the susceptibility of pigs to infections with Salmonella Typhimurium.deoxynivalenol / Salmonella Typhimurium / pig / macrophage / cytoskeleton

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Role of SPI-1 in the interactions of Salmonella Typhimurium with porcine macrophages

Cited by 29 publications

References 28 publications

Porcinein vitroandin vivomodels to assess the virulence ofSalmonella entericaserovar Typhimurium for pigs

Porcinein vitroandin vivomodels to assess the virulence ofSalmonella entericaserovar Typhimurium for pigs

Salmonella Typhimurium SPI-1 genes promote intestinal but not tonsillar colonization in pigs

The mycotoxin deoxynivalenol promotes uptake ofSalmonellaTyphimurium in porcine macrophages, associated with ERK1/2 induced cytoskeleton reorganization

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