Role of stearoyl-CoA desaturase-1 in skeletal muscle function and metabolism

Stamatikos, Alexis; Paton, Chad M.

doi:10.1152/ajpendo.00268.2013

Cited by 35 publications

(21 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…SCD1 desaturase indices are good biomarkers for actual enzyme activity and have also been proposed as a good clinical indicator of SCD1 gene expression. Hence, to assess SCD1 activity we calculated two different SCD1 indices by dividing the total concentration of 16:1 with 16:0 (SCD-16) and 18:1 with 18:0 (SCD-18) in each lipid fraction from serum and liver extracts [25, 27]. In the HFD group, the SCD-16 and SCD-18 indices were decreased in the liver TAG, DAG, and PL fractions as well as fasting systemic serum TAG (Fig.…”

Section: Resultsmentioning

confidence: 99%

High‐Fat Diet Alters Serum Fatty Acid Profiles in Obesity Prone Rats: Implications for InVitro Studies

Liu

Heden

Morris

et al. 2015

Lipids

View full text Add to dashboard Cite

High-fat diets (HFD) are commonly used in rodents to induce obesity, increase serum fatty acids, and induce lipotoxicity in various organs. In-vitro studies commonly utilize individual free fatty acids (FFA) to study lipid exposure in an effort to model what is occurring in-vivo, however, these approaches are not physiological as tissues are exposed to multiple fatty acids in-vivo. Here we characterize circulating lipids in obese-prone rats fed a HFD in both fasted and fed states with the goal of developing physiologically relevant fatty acid mixtures for subsequent in-vitro studies. Rats were fed a HFD (60% kcal fat) or a control diet (10% kcal fat) for 3 weeks; liver tissue, and both portal and systemic blood was collected. Fatty acid profiles and absolute concentrations of triglycerides (TAG) and FFA in the serum and TAG, diacylglycerol (DAG), and phospholipids (PL) in the liver were measured. Surprisingly, both systemic and portal serum TAG were ~40% lower in HFD-fed compared to controls. Overall, compared to the control diet, HFD feeding consistently induced an increase in the proportion of circulating polyunsaturated fatty acids (PUFA) with a concomitant decline in monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA) in both serum TAG and FFA. The elevations of PUFA were mostly attributed to increases in n-6 PUFA, linoleic acid and arachidonic acid. In conclusion, fatty acid mixtures enriched with linoleic and arachidonic acid in addition to SFA and MUFA should be utilized for in-vitro studies attempting to model lipid exposures that occur during in-vivo HFD condition.

show abstract

Section: Resultsmentioning

confidence: 99%

High‐Fat Diet Alters Serum Fatty Acid Profiles in Obesity Prone Rats: Implications for InVitro Studies

Liu

Heden

Morris

et al. 2015

Lipids

View full text Add to dashboard Cite

show abstract

“…De novo fatty acid synthesis (also referred to as de novo lipogenesis) occurs from the generation of C16:0 by the FASN in cytoplasm [42]. After the formation of palmitate, a series of chain elongations and desaturations occurs involving SCD and ELOVLs, to generate unsaturated or long-chain fatty acids [43,44]. A previous study reported that SCD can have a strong effect on fatty acid composition within skeletal muscle by converting SFA into MUFA [20].…”

Section: Expression Regulation Of Genes Involved In Lipogenesismentioning

confidence: 99%

Dynamic accumulation of fatty acids in duck (Anas platyrhynchos) breast muscle and its correlations with gene expression

Fan

Liu

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

Background: Fatty acid composition contributes greatly to the quality and nutritional value of meat. However, the molecular regulatory mechanisms underlying fatty acid accumulation in poultry have not yet been cleared. The aims of this study were to characterize the dynamics of fatty acid accumulation in duck breast muscle and investigate its correlations with gene expression. Results: Here, we analyzed the fatty acid profile and transcriptome of breast muscle derived from Pekin ducks and mallards at the ages of 2 weeks, 4 weeks, 6 weeks and 8 weeks. Twenty fatty acids were detected in duck breast muscle, with palmitic acid (C16:0, 16.6%~21.1%), stearic acid (C18:0, 9.8%~17.7%), oleic acid (C18:1n-9, 15.7%3 3.8%), linoleic acid (C18:2n-6, 10.8%~18.9%) and arachidonic acid (C20:4n-6, 11.7%~28.9%) as the major fatty acids. Our results showed that fatty acid composition was similar between the two breeds before 6 weeks, but the compositions diverged greatly after this point, mainly due to the stronger capacity for C16:0 and C18:1n-9 deposition in Pekin ducks. By comparing the multistage transcriptomes of Pekin ducks and mallards, we identified 2025 differentially expressed genes (DEGs). Cluster analysis of these DEGs revealed that the genes involved in oxidative phosphorylation, fatty acid degradation and the PPAR signaling pathway were upregulated in mallard at 8 weeks. Moreover, correlation analysis of the DEGs and fatty acid composition traits suggested that the DEGs involved in lipogenesis, lipolysis and fatty acid β-oxidation may interact to influence the deposition of fatty acids in duck breast muscle. Conclusions: We reported the temporal progression of fatty acid accumulation and the dynamics of the transcriptome in breast muscle of Pekin ducks and mallards. Our results provide insights into the transcriptome regulation of fatty acid accumulation in duck breast muscle, and will facilitate improvements of fatty acid composition in duck breeding.

show abstract

“…Reduced CD36 expression has been observed in the muscles of muscular dystrophy patients (Forst et al, 1998). SCD1 and SCD2 are integral membrane proteins of the endoplasmic reticulum and are implicated in the defective regulation of energy metabolism present in dystrophic muscle (Stamatikos et al, 2013). The increased expression of PPARg following tadalafil treatment seems to be specific to muscle tissue; it was also observed in an in vitro analysis on cultured myogenic cells.…”

Section: Discussionmentioning

confidence: 89%

Pathways Implicated in Tadalafil Amelioration of Duchenne Muscular Dystrophy

Arcangelis

Strimpakos

Gabanella

et al. 2015

Journal Cellular Physiology

View full text Add to dashboard Cite

Numerous therapeutic approaches for Duchenne and Becker Muscular Dystrophy (DMD and BMD), the most common X-linked muscle degenerative disease, have been proposed. So far, the only one showing a clear beneficial effect is the use of corticosteroids. Recent evidence indicates an improvement of dystrophic cardiac and skeletal muscles in the presence of sustained cGMP levels secondary to a blocking of their degradation by phosphodiesterase five (PDE5). Due to these data, we performed a study to investigate the effect of the specific PDE5 inhibitor, tadalafil, on dystrophic skeletal muscle function. Chronic pharmacological treatment with tadalafil has been carried out in mdx mice. Behavioral and physiological tests, as well as histological and biochemical analyses, confirmed the efficacy of the therapy. We then performed a microarray-based genomic analysis to assess the pattern of gene expression in muscle samples obtained from the different cohorts of animals treated with tadalafil. This scrutiny allowed us to identify several classes of modulated genes. Our results show that PDE5 inhibition can ameliorate dystrophy by acting at different levels. Tadalafil can lead to (1) increased lipid metabolism; (2) a switch towards slow oxidative fibers driven by the up-regulation of PGC-1α; (3) an increased protein synthesis efficiency; (4) a better actin network organization at Z-disk.

show abstract

Role of stearoyl-CoA desaturase-1 in skeletal muscle function and metabolism

Abstract: Stamatikos AD, Paton CM. Role of stearoyl-CoA desaturase-1 in skeletal muscle function and metabolism. Am J Physiol Endocrinol Metab 305: E767-E775,

Cited by 35 publications

References 91 publications

High‐Fat Diet Alters Serum Fatty Acid Profiles in Obesity Prone Rats: Implications for InVitro Studies

High‐Fat Diet Alters Serum Fatty Acid Profiles in Obesity Prone Rats: Implications for InVitro Studies

Dynamic accumulation of fatty acids in duck (Anas platyrhynchos) breast muscle and its correlations with gene expression

Pathways Implicated in Tadalafil Amelioration of Duchenne Muscular Dystrophy

Contact Info

Product

Resources

About