Role of Stromal-Derived Factor-1 in Mesenchymal Stem Cell Paracrinemediated Tissue Repair

Luo, Qing; Zhang, Bingyu; Kuang, Dongdong; Song, Guanbin

doi:10.2174/1574888x11666160614102629

Cited by 36 publications

(24 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 ). High-density cell injection at the topical region is also an obstacle for homing, thus using a vasodilator like heparin, culturing the cells under hypoxic condition, maintaining a lower confluence, or the addition of IL-3, IL-6, IGF-1, tumor necrosis factor alpha (TNF-α), or interferon-gamma (IFN-γ), can be used to increase C-X-C chemokine receptor type 4 (CXCR-4) [ 38 ], which is a specific receptor for stromal-derived-factor-1 (SDF-1, also called CXCL12), an MSC chemotactic factor that could improve homing efficiency [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model

et al. 2018

View full text Add to dashboard Cite

BackgroundMultipotent mesenchymal stem cells (MSCs) are used clinically in regenerative medicine. Our previous report showed systemically injected MSCs improved peri-implant sealing and accelerated tissue healing. However, the risks of systemic MSC administration, including lung embolism, must be considered; therefore, their local application must be assessed for clinical safety and efficacy. We investigated differences in treatment effect between local and systemic MSC application using a rat oral implantation model.MethodsRat bone marrow-derived MSCs were isolated and culture-expanded. The rat’s right maxillary first molars were extracted and replaced with experimental titanium implants. After 24 h, MSCs (1 × 106/ml) were systemically or locally injected into recipient rats via the tail vein (systemic group) or buccal subcutaneous tissue (local group), respectively. Rats treated in the absence of MSCs were included as a control (control group). The maxillary epithelium was assessed histologically after 4 weeks to evaluate laminin-332 (Ln-332) distribution and horseradish peroxidase invasion, as indicators of peri-implant epithelium (PIE) formation and PIE sealing to the implant surface, respectively. The effect of MSCs on rat oral epithelial cell (OEC) morphology was determined by coculture.ResultsSystemic group MSCs accumulated early at the peri-implant mucosa, while local group MSCs were observed in various organs prior to later accumulation around the implant surface. PIE formation and Ln-332-positive staining at the implant interface were enhanced in the systemic group compared with the local and control groups. Furthermore, OEC adherence on implants was reduced in high-density compared with low-density MSC cocultures.ConclusionsLocal MSC injection was more ineffective than systemic MSC injection at enhancing PIE sealing around titanium implants. Thus, although local MSC administration has a wide range of applications, further investigations are needed to understand the exact cellular and molecular mechanisms of this approach prior to clinical use.

show abstract

Section: Resultsmentioning

confidence: 99%

The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model

et al. 2018

View full text Add to dashboard Cite

show abstract

“…TSG-6 has anti-inflammatory action and, furthermore, a specific inhibitory function against MMPs [ 30 ]. Finally, SDF-1 and its receptor are key players in the recruitment of MSCs and endothelial cells in inflamed, as well as in damaged tissues [ 31 , 32 ]. Equine aMSCs express STC-1, IL1-Ra, TSG-6, and SDF-1.…”

Section: Discussionmentioning

confidence: 99%

The Contribution of Adipose Tissue-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma to the Treatment of Chronic Equine Laminitis: A Proof of Concept

Angelone

Conti

Biacca

et al. 2017

IJMS

View full text Add to dashboard Cite

Laminitis, a highly debilitating disease of the foot in ungulates, is characterized by pathological changes of the complex lamellar structures that maintain the appendicular skeleton within the hoof. Laminitis is a multifactorial disease that involves perturbation of the vascular, hematological, and inflammatory homeostasis of the foot. Interestingly, the pathogenesis of the disease resembles what is observed in metabolic syndromes and sepsis-induced organ failure in humans and animals. We hypothesized that local administration of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) might contribute to establishing an anti-inflammatory and pro-angiogenic environment, and could stimulate the injured tissue in order to restore its functional integrity. According to this assumption, an experimental protocol based on the local intravenous administration of adipose tissue-derived MSCs (aMSCs) in combination with PRP was developed for the treatment of horses affected by chronic laminitis. Nine horses with severely compromised venograms (showing grade III and IV laminitis) that had been unsuccessfully treated with conventional therapies were enrolled. aMSCs and PRP (15 × 106 cells resuspended in 15 mL of PRP) were injected into the lateral or medial digital vein three times, at one-month intervals. The first administration was performed with allogeneic aMSCs, while for the following administrations, autologous aMSCs were used. There was no adverse short-term reaction to the intravenous injection of aMSCs. In the long term, venograms outlined, in all subjects, a progressive amelioration of the vascularization of the foot. An improvement in the structure and function of the hoof was also observed. No adverse events were reported during the follow-up, and the horses returned to a comfortable quality of life. Although the number of animals enrolled in the study is limited, both clinical observations and venography demonstrated an enhancement in the condition of all horses, suggesting that the regenerative therapies in chronic laminitis could be useful, and are worthy of further investigation.

show abstract

“…Studies have demonstrated the potential role of MSCs in various biological processes like contributing to cell generation, survival, and alteration of cellular phenotype [27][28][29] . MSCs govern the repair mechanisms occurring at cellular and molecular levels by communication through the release of different factors mostly proteins 30 . In this study, we investigated the potential role of MSCs in the repair of the plasma membrane and chromatin material of dog sperm following cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

Improved viability and fertility of frozen-thawed dog sperm using adipose-derived mesenchymal stem cells

Qamar

Fang

Kim

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Cryopreservation procedures negatively affect the quality traits of sperm, causing certain changes at structural and molecular levels due to thermal, mechanical, osmotic, and oxidative damage. The objective of this study was to examine the potential of canine adipose-derived mesenchymal stem cells (Ad-MSCs) for providing protection to the dog sperm against cryo-damage. Canine Ad-MSCs were selected on the basis of the significantly higher gene expression for different proteins actively involved in the cell repair including annexin 1 (ANX1), histone H3 (H3) and high mobility group B (HMGB) protein compared to skin fibroblasts. Semen was collected from four healthy dogs by digital manipulation. The washed pooled ejaculates were diluted with buffer 2 (extender) supplemented without Ad-MSCs (Control), with 2.5 × 10 6 Ad-MSCs/mL (Group 1) or with 5 × 10 6 Ad-MSCs/mL (Group 2). Group 1 exhibited significantly higher post-thaw motility, live sperm, intact plasma membrane and normal acrosomes than the other groups. Additionally, Group 1 showed significantly higher expression levels of genes related to the repair of membranes (ANX1, dysferlin; DYSF, and fibronectin; FN1) and chromatin material (H3 and HMGB). Protein expression of ANX1, H 3, and FN1 was also statistically more in Group 1 than in Control. The results confirm that canine Ad-MSCs can effectively preserve the quality of frozenthawed sperm by a reduction in cryoinjury. At an appropriate concentration, Ad-MSCs significantly improve the quality of post-thaw dog sperm. Semen cryopreservation is regarded as the most important step of artificial insemination which is the most widely adopted assisted reproductive technology (ART) in canine practice 1. It can facilitate the storage of the genetic material for an extended period, conserve the elite individual's fertility and serve as a useful tool for preserving the endangered species. In addition, it has greatly benefited the animal-based industry by reducing the cost and the stress associated with transportation, overcoming the quarantine restrictions and breeding issues (e.g., aggressive behavior and size issues) 2. Despite many attempts to improve freezing agents and techniques, reduction in semen quality still remains the major issue associated with freezing procedures. During freezing the quality-related traits of sperm are highly compromised 3. Sperm undergoes certain detrimental changes at the structural and molecular levels as a result of thermal, mechanical, osmotic, and oxidative damage 4,5. The sperm that survives during freezing procedure suffers a reduction in fertility 6 and this has been linked with damage that adversely affects viability, motility, plasma membrane, acrosome and chromatin material 7. In addition, the activation of apoptotic pathways results in the fragmentation of sperm cell DNA 8. These changes ultimately contribute to an overall reduction in the fertility of sperm. Current protective modifications employed to minimize the above mentioned damaging effects involve the use of different ty...

show abstract

Role of Stromal-Derived Factor-1 in Mesenchymal Stem Cell Paracrinemediated Tissue Repair

Cited by 36 publications

References 0 publications

The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model

The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model

The Contribution of Adipose Tissue-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma to the Treatment of Chronic Equine Laminitis: A Proof of Concept

Improved viability and fertility of frozen-thawed dog sperm using adipose-derived mesenchymal stem cells

Contact Info

Product

Resources

About