Role of surface layer collagen binding protein from indigenous Lactobacillus plantarum 91 in adhesion and its anti-adhesion potential against gut pathogen

Abstract

Human feacal isolates were ascertain as genus Lactobacillus using specific primer LbLMA1/R16-1 and further identified as Lactobacillus plantarum with species specific primers Lpl-3/Lpl-2. 25 L. plantarum strains were further assessed for hydrophobicity following the microbial adhesion to hydrocarbons (MATH) method and colonization potentials based on their adherence to immobilized human collagen type-1. Surface proteins were isolated from selected L. plantarum 91(Lp91) strain. The purified collagen binding protein (Cbp) protein was assessed for its anti-adhesion activity against enteric Escherichia coli 0157:H7 pathogen on immobilized collagen. Four L. plantarum strains displayed high degree of hydrophobicity and significant adhesion to collagen. A 72 kDa protein was purified which reduced 59.71% adhesion of E. coli 0157:H7 on immobilized collagen as compared to control well during adhesion assay. Cbp protein is the major influencing factor in inhibition of E. coli 0157:H7 adhesion with extracellular matrix (ECM) components. Hydrophobicity and adhesion potential are closely linked attributes precipitating in better colonization potential of the lactobacillus strains. Cbp is substantiated as a crucial surface protein contributing in adhesion of lactobacillus strains. The study can very well be the platform for commercialization of indigenous probiotic strain once their functional attributes are clinically explored.

Insight into Potential Probiotic Markers Predicted in Lactobacillus pentosus MP-10 Genome Sequence

“…Furthermore, we predicted the presence of collagen-binding protein specific to L. pentosus MP-10, which could be involved in their adhesion to epithelial cells/extracellular matrix proteins similarly as shown other lactobacilli such as L. reuteri NCIB 11951 (Roos et al, 1996) and L. fermentum RC-14 (Heinemann et al, 2000). Thus, this could be of vital importance for effective colonization and also competitive displacement of gut pathogens (Yadav et al, 2013). …”

Bacteriocin-producing strains of Lactobacillus plantarum inhibit adhesion of Staphylococcus aureus to extracellular matrix: quantitative insight and implications in antibacterial therapy

“…The presence of cnbp suggested the potential of L. plantarum strains to adhere onto collagen. Collagen-binding protein of L. plantarum has been previously reported to be instrumental in adhesion and in reducing the binding of gut pathogens to collagen (Yadav et al, 2013).…”

“…Adhesion to the extracellular matrix (ECM) and intestinal cells is a key attribute of probiotic strains (Lebeer et al, 2010;Lee et al, 2004;Reid & Burton, 2002). Certain lactobacilli have been shown to have an inclination to adhere to ECM molecules such as collagen and mucin, and can thus be applied for inhibition of pathogen adhesion (Lorca et al, 2002;Miyoshi et al, 2006;Muñoz-Provencio et al, 2009;Ouwehand et al, 2001;Vélez et al, 2007;Vesterlund et al, 2005;Yadav et al, 2013). Furthermore, mucin-binding lactobacilli have been shown to inhibit attachment of opportunistic pathogens and this is noteworthy as several enteric pathogens have evolved mechanisms to breach the mucosal barrier (McGuckin et al, 2011;Shanahan, 2010).…”

HIV-induced immunosuppression is associated with colonization of the proximal gut by environmental bacteria

“…The mechanism that protects the host from invasion by exogenous bacteria is termed colonization resistance [1]. Endogenous bacteria can directly preclude exogenous bacteria from stable colonization by competing for essential nutrients [2] and mucosal attachment sites [3], and by inhibiting the growth of exogenous bacteria through the secretion of bacteriocin [4]. Besides endogenous bacteria, the host immune system also plays a critical role in maintaining a stable microbiome.…”