Role of suture diameter and vessel insertion position in the establishment of the middle cerebral artery occlusion rat model

Abstract

The aim of the present study was to explore the role of suture diameter and vessel insertion position in the preparation of the middle cerebral artery occlusion (MCAO) rat model. A total of 84 Sprague-Dawley rats (weighing 250–300 g) were randomly divided to three groups: group A (type 1.0, suture diameter 0.16–0.17 mm and tip 0.21–0.22 mm); group B (type 2.0; suture diameter, 0.22–0.23 mm; tip, 0.27–0.28 mm); and group C (type 3.0; suture diameter, 0.28–0.29 mm; and tip, 0.33–0.34 mm). The animals in each group were then subdivided into two subgroups, one of which received a nylon line inserted through the external carotid artery (ECA insertion), while the other received the nylon line through the common carotid artery (CCA insertion) subsequent to a middle or lateral neck incision. The neurological deficit score was evaluated at 4, 8, 24, 48 and 72 h post-surgery. The ischemic brain tissue was stained by 2,3,5-triphenyltetrazolium chloride (TTC) to evaluate the extent of the infarct volume. The cerebral edema rate, cerebral infarction volume rate, relative standard deviation (RSD) of the cerebral infarction rate and the success rate were also assessed. The rectal temperature, PaO2, PaCO2, pH, blood pressure and blood glucose levels were controlled and did not vary between the group types. The results suggested that suture diameter and insertion route affected the infarct volume and success rate in the establishment of the suture MCAO rat model. Furthermore, the MCAO model with a 0.22–0.23 mm diameter suture and CCA insertion route provided the highest success rate in the SD rats.

Pulling wire to avoid misinsertion of filament and vascular damage improves ischemic stroke models' success rate and repeatability

“…28 The depth of the plug also affects the success rate of the model, and in our experience, a depth of 18-20 mm is appropriate for the insertion of the plug, which is just sufficient to occlude the MCA. 29 Therefore, a silicone coated filament with a 2 cm marker point in the middle section (3400A Western Thickening) was used to easily determine the depth of filament insertion and improve the success rate of modeling. It is worth noting that the time of ischemia-reperfusion also plays an important role in the success of the MCAO model, which is usually 60, 90, or 120 minutes.…”

Comprehensive Analysis of the Effect of 20(R)-Ginsenoside Rg3 on Stroke Recovery in Rats via the Integrative miRNA–mRNA Regulatory Network

“…In order to reduce animal mortality and ensure the stability and reliability of the MCAO/R model, we selected rats with a body weight of 280–320 g [ 20 ]. Male grade SPF Sprague–Dawley rats were purchased from the Laboratory Animal Center of Kunming Medical University (license number: SCXK (Yunnan) k2015-0002, Yunnan, China).…”

Dynamic assessment of brain perfusion in a middle cerebral artery occlusion rat model by contrast-enhanced ultrasound imaging: a pilot study

“…To date, inconsistencies in ischemic severity or lesion volume have greatly limited the potential utility of the model in preclinical studies. Numerous factors may affect the consistency of middle cerebral artery occlusion (MCAo), such as animal strain, surgery procedure, physiological variables, premature perfusion, subarachnoid hemorrhage, or inadvertent occlusion of other arteries, such as anterior choroidal artery (4)(5)(6)(7)(8)(9). Individual differences of collateral compensation in different rats is another important reason (6,(9)(10)(11).…”