Role of Synadenium grantii latex proteases in nematicidal activity on Meloidogyne incognita and Panagrellus redivivus

Abstract

Synadenium grantii is a Euphorbiaceae plant commonly found in Brazil, known as Janaúba or Leitosinha, whose latex is traditionally used for several purposes. However, it is not known whether the nematicidal action of this plant latex occurs due to the action of proteases. The present work aims to evaluate the nematicidal activity of proteases from Synadenium grantii latex on Meloidogyne incognita and Panagrellus redivivus. S. grantii latex used in the present study was collected from specimens found in Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil. The drained latex was collected in Eppendorf microtubes and immediately stored on ice at 4 °C. After this extraction, the latex was frozen (-20 °C) during 2 hours, thawed at room temperature (25 °C) and centrifuged at 10,000 g at 4 °C for 30 minutes to remove larger particles and concentrate the proteases. After the centrifugation, assays of enzymatic activity were performed in order to know in which of the phases the enzymes were found. S. grantii latex presented protease, but no chitinase activity. The results show that there was a significant difference (p <0.01) between the treated and control groups, with 100% mortality of Meloidogyne incognita and 72% average mortality of Panagrellus redivivus. In addition, it was demonstrated that the nematicidal action occurred due to the action of the proteases, since the control was only differentiated from the treatment by the presence of the enzymes with biological activity.

Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita

“…The efficiency of E. trigona crude extract in nematodes reduction is showed by the high reduction percentage observed, with 96% reduction in 24 hours of treatment, when compared to control (Figure 3). In a similar study, the proteases from Synadenium grantii reduced the number of M. incognita juveniles in 100% after 24 hours of treatment (Gomes et al, 2019). The present study is the first report of E. trigona latex with nematicidal activity, therefore more studies regarding latex proteases action on nematodes are needed.…”

Nematicidal activity of Brazilian honeys

“…These free-living nematodes are excellent models as they are similar to parasitic species (Srinivasan et al, 2013;Burns et al 2015). In previous studies, nematodes of the genus Panagrellus were successfully used as a model for evaluating nematicidal activity (Soares et al, 2015;Gomes et al, 2018;Ferreira et al, 2019). Thus, a significant nematicidal activity against these organisms points to a great potential for use on Research, Society andDevelopment, v. 12, n. 4, e15712440989, 2023 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v12i4.40989 parasitic nematodes.…”

Cost-effective bacteria-based bionematicide formula to control the root-knot nematode Meloidogyne spp. on tomato plants

“…population in the soil is protease. Protease enzyme was reported to be capable of degrading root-knot nematode cuticle and eggs that are composed of proteins (De Souza Gouveia et al 2017;Gomes et al 2019). Safni et al (2018) reported that bacteria with the capability of producing chitinase and protease enzymes could in vitro lyse Meloidogyne spp.…”