Role of T cell subsets during the recall of immunologic memory to <i>Leishmania major</i>

Müller, Ingrid

doi:10.1002/eji.1830221206

Cited by 56 publications

(45 citation statements)

References 56 publications

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“…[21][22][23] These cells respond to recall antigens and can be detected in two ways: 1) by production of IFN-γ, 21 which can be assessed in vitro, and 2) by the LST, which shows an in vivo DTH reaction induced by intradermal injection of leishmanin. Although these reactions can be observed after curing of leishmaniasis when the host is generally immune, these responses do not always indicate protection because abundance of the Th2-type response in the presence of the Th1-response leads to persistent disease, as in the chronic non-healing form of CL or mucosal leishmaniasis, where IFN-γ and LST reactivities are high.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Interferon-γ Levels and Leishmanin Skin Test Results in Persons Recovered for Leishmaniasis

Alimohammadian

Jones²,

Darabi³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Patients who recover from leishmaniasis usually show development of strong immunity and induction of interferon-γ (IFN-γ) and delayed type hypersensitivity. In a randomized trial, we analyzed the IFN-γ response by using a Quantiferon-Leishmania assay against three Leishmania peptide antigens and compared it with results of the leishmanin skin test (LST) in persons residing in areas in Iran to which zoonotic cutaneous leishmaniasis (ZCL, 181 persons), anthroponotic cutaneous leishmaniasis (ACL, 104 persons), and zoonotic visceral leishmaniasis (ZVL, 67 persons) are endemic. The percentage of persons with an IFN-γ-positive response ( 0.2 IU/mL) to three antigens and the mean concentration of IFN-γ induced by the antigens were higher for persons from areas endemic for ZVL than for persons from areas endemic for ZCL and ACL. The percentage of persons with LST-positive results ( 5 mm indurations) was 99%, 94%, and 70% for areas with ZCL, ACL, and ZVL, respectively. Our data indicate that the LST is significantly more sensitive than IFN-γ levels in persons who have been cured of cutaneous leishmaniasis than in persons who have been cured of ZVL.

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Section: Discussionmentioning

confidence: 99%

Assessment of Interferon-γ Levels and Leishmanin Skin Test Results in Persons Recovered for Leishmaniasis

Alimohammadian

Jones²,

Darabi³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…The biological consequence of these different abilities of DC subtypes to present MHC class I-bound Ags is currently not easy to define. Even though adaptive immunity against L. major parasites is mediated by CD4 ϩ Th1 cells, CD8 ϩ T cells are likely involved in promoting protection toward secondary infections (46). Furthermore, CD8…”

Section: Discussionmentioning

confidence: 99%

Priming of CD8+ and CD4+ T Cells in Experimental Leishmaniasis Is Initiated by Different Dendritic Cell Subtypes

Brewig

Kissenpfennig

Malissen

et al. 2009

The Journal of Immunology

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The biological role of Langerin+ dendritic cells (DCs) such as Langerhans cells and a subset of dermal DCs (dDCs) in adaptive immunity against cutaneous pathogens remains enigmatic. Thus, we analyzed the impact of Langerin+ DCs in adaptive T cell-mediated immunity toward Leishmania major parasites in a Lang-DTR mouse model that allows conditional diphtheria toxin (DT)-induced ablation of Langerin+ DCs in vivo. For the first time, infection experiments with DT-treated Lang-DTR mice revealed that proliferation of L. major-specific CD8+ T cells is significantly reduced during the early phase of the immune response following depletion of Langerin+ DCs. Consequently, the total number of activated CD8+ T cells within the draining lymph node and at the site of infection is diminished. Furthermore, we show that the impaired CD8+ T cell response is due to the absence of Langerin+ dDCs and not Langerhans cells. Nevertheless, the CD4+ T cell response is not altered and the infection is cleared as effectively in DT-treated Lang-DTR mice as in control mice. This clearly demonstrates that Langerin+ DCs are, in general, dispensable for an efficient adaptive immune response against L. major parasites. Thus, we propose a novel concept that, in the experimental model of leishmaniasis, priming of CD4+ T cells is mediated by Langerin− dDCs, whereas Langerin+ dDCs are involved in early priming of CD8+ T cells.

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“…Furthermore, reconstitution of resistance in RAG Ϫ/Ϫ mice using T cells from naive C57BL/6 mice was optimal only when both CD4 ϩ and CD8 ϩ T cells were transferred. In the conventional high dose, s.c. infection model, a role for CD8 ϩ T cells in resistance to reinfection by L. major has already been established (12). CD8…”

Section: Discussionmentioning

confidence: 99%

“…Acquired resistance in this model has been shown to require CD4 ϩ T cells, activated in an IL-12-driven and CD40L-dependent manner, for the production of high levels of IFN-␥ that induce NO-dependent killing by infected macrophages (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). Although CD8 ϩ T cells were shown to be essential to immunity to reinfection in resistant mice that healed their primary lesion (11)(12)(13)(14), the role of CD8 ϩ T cells in the control of primary infection remains unclear. A higher frequency of parasite-specific CD8 ϩ T cells was found in resistant (C57BL/6) compared with susceptible (BALB/c) mice (2,11).…”

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confidence: 99%

CD8+ T Cells Are Required for Primary Immunity in C57BL/6 Mice Following Low-Dose, Intradermal Challenge withLeishmania major

Belkaid

Stebut

Méndez

et al. 2002

The Journal of Immunology

281

253

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Standard murine models of cutaneous leishmaniasis, involving s.c. inoculation of large numbers of Leishmania major promastigotes, have not supported an essential role for CD8+ T cells in the control of primary infection. Recently, a L. major model combining two main features of natural transmission, low parasite dose and inoculation into a dermal site, has been established in resistant C57BL/6 mice. In the present studies, C57BL/6 mice with CD8+ T cell deficiencies, including CD8−/− and CD8-depleted mice, failed to control the growth of L. major following inoculation of 100 metacyclic promastigotes into the ear dermis. The resulting dermal pathology was minor and delayed. Lesion formation in wild-type mice was coincident with the killing of parasites in the inoculation site. Both events were associated with the accumulation of CD8+ T lymphocytes in the skin and with the capacity of CD8+ T cells recovered from draining lymph nodes or infected dermis to release IFN-γ following coculture with infected dendritic cells. Reconstitution of resistance to L. major in RAG−/− mice using T cells from naive donors was optimal when both CD4+ and CD8+ T cells were transferred. Primed CD8+ T lymphocytes obtained from C57BL/6 mice during the acute stage of infection were able to mediate both pathology and immunity when transferred alone. The low dose, intradermal challenge model reveals that CD8+ T cells play an essential role in both pathogenesis of and immunity to primary infection with L. major in the skin.

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Role of T cell subsets during the recall of immunologic memory to Leishmania major

Cited by 56 publications

References 56 publications

Assessment of Interferon-γ Levels and Leishmanin Skin Test Results in Persons Recovered for Leishmaniasis

Assessment of Interferon-γ Levels and Leishmanin Skin Test Results in Persons Recovered for Leishmaniasis

Priming of CD8+ and CD4+ T Cells in Experimental Leishmaniasis Is Initiated by Different Dendritic Cell Subtypes

CD8+ T Cells Are Required for Primary Immunity in C57BL/6 Mice Following Low-Dose, Intradermal Challenge withLeishmania major

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