Role of the Endothelial Layer in the Deswelling Process of Organ-Cultured Human Corneas Before Transplantation

Schnitzler, Anne Christine; Salla, Sabine; Hamsley, Nicole; Flammersfeld, Ansgar; Fuest, Matthias; Walter, Peter; Hermel, Martin

doi:10.1097/ico.0000000000000937

Cited by 11 publications

(12 citation statements)

References 17 publications

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“…All three corneas followed the same thickness profile, with an initially rapid decrease for approximately 3 hours caused by the osmotic de-swelling due to the hypertonic dextran solution. After this minimum, there was a slower increase in the thickness of the cornea [ 19 ], which was associated with dextran diffusing into the cornea [ 44 ], changing the osmotic balance. The temporal sampling period of the thickness measurements of de-swelling corneas, by this automated method, is much smaller (1 versus 30 minutes [ 30 ]) than has previously been presented.…”

Section: Resultsmentioning

confidence: 99%

“…To counteract this, donor corneas are “de-swelled” before use in a hypertonic solution, usually containing dextran, which removes the excess water from the cornea by osmosis. The health of the endothelial layer affects the de-swelling rate [ 19 ] and conversely, the de-swelling process can damage the health of the donor tissue [ 20 ]. For these reasons there has been substantial research to optimise the de-swelling time [ 9 , 21 ], improve the method [ 10 ], or use alternative osmotic agents such as poloxamers or PEG [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Deformation velocity imaging using optical coherence tomography and its applications to the cornea

Lawman¹,

Madden²,

Romano³

et al. 2017

Biomed. Opt. Express

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Optical coherence tomography (OCT) can monitor human donor corneas non-invasively during the de-swelling process following storage for corneal transplantation, but currently only resultant thickness as a function of time is extracted. To visualize and quantify the mechanism of de-swelling, we present a method exploiting the nanometer sensitivity of the Fourier phase in OCT data to image deformation velocities. The technique was demonstrated by non-invasively showing during de-swelling that osmotic flow through an intact epithelium is negligible and removing the endothelium approximately doubled the initial flow at that interface. The increased functional data further enabled the validation of a mathematical model of the cornea. Included is an efficient method of measuring high temporal resolution (1 minute demonstrated) corneal thickness, using automated collection and semi-automated graph search segmentation. These methods expand OCT capabilities to measure volume change processes for tissues and materials.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Deformation velocity imaging using optical coherence tomography and its applications to the cornea

Lawman¹,

Madden²,

Romano³

et al. 2017

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…23 Other investigations employed OCT-based methods because they enable the measurement of corneoscleral buttons under sterile conditions. [24][25][26][27] LASIK interfaces could be detected within the reflection pattern of the stromal tissue. Beyond that, Lin et al estimated anterior and posterior curvatures by means of a parabolic fit.…”

Section: Introductionmentioning

confidence: 91%

“…The deswelling of organ-cultured corneal tissue was investigated by Schnitzler et al who found that the condition of the endothelium might have a significant effect on the deswelling aside from the osmotic effect of the culture medium. 27 Thus, measuring corneal thickness before reimmersement and at a standardized time after reimmersement might be one indicative factor for endothelium activity.…”

Section: Corneal Parametersmentioning

confidence: 99%

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Optical coherence tomography-based topography determination of corneal grafts in eye bank cultivation

et al. 2017

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Abstract. Vision loss due to corneal injuries or diseases can be treated by transplantation of human corneal grafts (keratoplasty). However, quality assurance in retrieving and cultivating the tissue transplants is confined to visual and microbiological testing. To identify previous refractive surgery or morphological alterations, an automatic, noncontact, sterile screening procedure is required. Twenty-three corneal grafts have been measured in organ culture with a clinical spectral-domain optical coherence tomographer. Employing a biconic surface fit with 10 degrees of freedom, the radii of curvature and conic constants could be estimated for the anterior and posterior corneal surfaces. Thereupon, central corneal thickness, refractive values, and astigmatism have been calculated. Clinical investigations are required to elaborate specific donor-host matching in the future.

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Anterior Segment OCT: Application to Improve Graft Selection for Corneal Transplantation

Seitz

Asi

Mäurer

et al. 2020

Essentials in Ophthalmology

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Role of the Endothelial Layer in the Deswelling Process of Organ-Cultured Human Corneas Before Transplantation

Abstract: Corneal dehydration after organ culture seems to be a multifactorial process, which not only depends on osmotic effects of the deswelling compound but also requires the presence of an intact endothelial cell layer.

Cited by 11 publications

References 17 publications

Deformation velocity imaging using optical coherence tomography and its applications to the cornea

Deformation velocity imaging using optical coherence tomography and its applications to the cornea

Optical coherence tomography-based topography determination of corneal grafts in eye bank cultivation

Anterior Segment OCT: Application to Improve Graft Selection for Corneal Transplantation

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