Role of the equine CYP3A94, CYP3A95 and CYP3A97 in ketamine metabolism in presence of medetomidine, diazepam and methadone studied by enantioselective capillary electrophoresis

Sandbaumhüter, Friederike Andrea; Vimercati, Sara Laura; Thormann, Wolfgang; Mevissen, Meike

doi:10.1016/j.tiv.2018.03.016

Cited by 7 publications

(10 citation statements)

References 35 publications

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“…No reaction was observed in the presence of canine or human liver microsomes as well as purified uridine‐5'‐diphospho‐glucuronosyl‐transferase. A chiral CE method was used for the investigation of the kinetics of the in vitro demethylation of ketamine to norketaime by the equine cytochrome P450 (CYP) isoenzymes CYP3A94, CYP3A95, and CYP3A97 as well as equine liver microsomes [142]. The effect of the benzodiazepine diazepam, the α 2 ‐receptor agonist medetomidine, and the opiate methadone on the ketamine metabolism was also studied.…”

Section: Ce In Stereoisomer Analysis Of Pharmaceuticalsmentioning

confidence: 99%

Advances of capillary electrophoresis enantioseparations in pharmaceutical analysis (2017–2020)

2021

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Capillary electrophoresis is a powerful technique for the analysis of polar chiral compounds and has been widely accepted for analytical enantioseparations of drug compounds in pharmaceuticals and biological media. In addition, many mechanistic studies have been conducted in an attempt to rationalize enantioseparations in combination with spectroscopic and computational techniques. The present review will focus on recent examples of mechanistic aspects and summarize recent applications of stereoselective pharmaceutical and biomedical analysis published between January 2017 and November 2020. Various separation modes including electrokinetic chromatography in combination with several detection modes including laser-induced fluorescence, mass spectrometry and contactless conductivity detection will be discussed. A general trend also observed in other analytical techniques is the application of quality by design principles in method development and optimization.

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Section: Ce In Stereoisomer Analysis Of Pharmaceuticalsmentioning

confidence: 99%

Advances of capillary electrophoresis enantioseparations in pharmaceutical analysis (2017–2020)

2021

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“…Drug metabolism studies in presence of fungi were undertaken with albendazole [66], risperidone [67], propranolol [68], midodrine [69], zopiclone [70], hydroxyzine [71], and oxcarbazepine [72]. The fate of ketamine [46,60,76,79–81,83,84] and tetrabenazine [82] were also studied in presence of inhibitors or drugs that are typically coadministrated in clinical practice.…”

Section: Applicationsmentioning

confidence: 99%

“…The use of a recombinant single CYP enzyme instead of organ microsomes provides information about the possible involvement of a specific enzyme in a metabolic step together with its stereospecificity when an appropriate chiral assay is used. Using chiral CE, this was successfully shown for CYP enzymes involved in the metabolism of fluoxetine [74], verapamil [75], ketamine [76][77][78][79][80][81]83], and norketamine [78,81]. Liver microsomes were incubated with ketamine [46,47,60,73,78,80,83,84], norketamine [47,78], tetrabenazine [82], and fenbendazole [62].…”

Section: Assessment Of Drug Metabolism and Inhibition In Vitromentioning

confidence: 99%

“…In this article, CE‐based assays and achievements reported in the 2010 to 2020 literature are comprehensively but not exhaustively reviewed. The main focus is on real samples including enantiomer analysis of drugs and metabolites in body fluids and tissues of humans and animals (Tables 1 and 2, [31–58]) and in in vitro preparations (Tables 3 and 4, [46,47,59–84]). In contrast to previous reviews from our laboratory [26,30], articles dealing with enantiomer analysis in fortified body fluids and tissues are not listed.…”

Section: Introductionmentioning

confidence: 99%

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Bioanalysis of drugs and their metabolites by chiral electromigration techniques (2010–2020)

Caslavska

Thormann

2021

Electrophoresis

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The further development and application of capillary electromigration techniques for the enantioselective determination of drugs and their metabolites in body fluids, tissues, and in vitro preparations during the 2010 to 2020 time period continued to proof their usefulness and attractiveness in bioanalysis. This review discusses the principles and important aspects of capillary electrophoresis‐ based chiral drug bioassays, provides a survey of the assays reported during the past 10 years and presents an overview of the key achievements encountered in that time period. For systems with charged chiral selectors, special attention is paid on assays that feature field‐amplified sample injection to enable the determination of ppb levels of analytes and optimized online incubation procedures for the rapid assessment of a metabolic pathway. Applications discussed encompass the pharmacokinetics of drug enantiomers in vivo and in vitro, the impact of inhibitors on metabolic steps, the elucidation of the stereoselectivity of drug metabolism in vivo and in vitro, and drug enantiomers in toxicological, forensic, and doping analysis.

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“…Therefore, the development of an efficient and reliable method for the analysis of the drugs in different samples like pharmaceutical preparations, clinical or criminal examinations and biological fluids is crucial for the analysts. 6 Up to now, diverse approaches such as capillary electrophoresis, 7,8 gas chromatography (GC), 9,10 and high performance liquid chromatography [11][12][13][14][15] have been developed for the determination of these types of drugs. Among these methods, chromatographic techniques are preferred due to the possibility of simultaneous determination of several anti-seizures in different samples.…”

Section: Introductionmentioning

confidence: 99%

Development of Salt Induced Liquid–Liquid Extraction Combined with Amine Based Deep Eutectic Solvent-Dispersive Liquid–Liquid Microextraction; An Efficient Analytical Method for Determination of Three Anti-Seizures in Urine Samples

et al. 2020

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Background: A simple, fast, efficient, and environmentally friendly microextraction technique named salt induced liquid-liquid extraction combined with amine based deep eutectic solventdispersive liquid-liquid microextraction method followed by gas chromatography-flame ionization detector was applied for the determination of some anti-seizure drugs in urine samples. Methods: In this method, sodium chloride (30% w/v) was dissolved in the sample solution (adjusted at pH=10) as phase separation agent and iso-propanol was added to the solution. After manually shaking, the solution was centrifuged and the supernatant phase was removed and mixed with choline chloride: benzyl ethylenediamine (85 µL) and the mixture was rapidly dispersed into a deionized water containing 5.0 % w/v, sodium chloride and adjusted at pH=10. The cloudy solution was centrifuged and the sedimented phase was removed and 1 µL of it was injected into the determination system. Results: Under final conditions, the limits of detection and quantification at the ranges of 3.4-6.9, and 11.5-23.3 ng mL -1 were obtained, respectively. The relative standard deviations for intraand inter day assays were lower than 10.9%. The effect of exogenous and endogenous effect on the performance of the method was studied and the results showed that these factors were nearly effect less on the efficiency. Conclusion:The introduced method was satisfactorily utilized to determinate the selected benzodiazepines drugs in the patients' urine samples.

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Role of the equine CYP3A94, CYP3A95 and CYP3A97 in ketamine metabolism in presence of medetomidine, diazepam and methadone studied by enantioselective capillary electrophoresis

Cited by 7 publications

References 35 publications

Advances of capillary electrophoresis enantioseparations in pharmaceutical analysis (2017–2020)

Advances of capillary electrophoresis enantioseparations in pharmaceutical analysis (2017–2020)

Bioanalysis of drugs and their metabolites by chiral electromigration techniques (2010–2020)

Development of Salt Induced Liquid–Liquid Extraction Combined with Amine Based Deep Eutectic Solvent-Dispersive Liquid–Liquid Microextraction; An Efficient Analytical Method for Determination of Three Anti-Seizures in Urine Samples

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