1992
DOI: 10.1128/jb.174.3.743-748.1992
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Role of the heat shock response in stability of mRNA in Escherichia coli K-12

Abstract: The heat shock response in Escherichia coli involves extensive induction of the heat shock proteins, with the concomitant suppression of the synthesis of the non-heat shock proteins. While the induction of the heat shock proteins has been shown to occur primarily at the transcriptional level, the suppression of non-heat shock proteins is poorly understood. We have investigated the possibility that an increased decay of non-heat shock mRNAs is a means of decreasing the synthesis of non-heat shock proteins durin… Show more

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Cited by 25 publications
(17 citation statements)
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“…Thus the use of strains containing mutations in PNPase (pnp), RNase II (rnb) and RNase E (rne) has been very helpful for studying mRNA degradation. In particular, SK5704 (pnp-7 rnb-500 rne-1) and its derivatives have been employed extensively to examine the decay of several monocistronic and portions of polycistronic transcripts (Alifano et al, 1994;Arraiano et al, 1988Arraiano et al, , 1993Hajnsdorf et al, 1994;Henry et al, 1992;Mackie, 1991). In this genetic background, upon shift to elevated temperatures, the strain becomes de®cient in PNPase, RNase II and RNase E and, for many mRNAs, decay intermediates are stabilized.…”
Section: Pyrf-orff Transcript Decaymentioning
confidence: 99%
See 1 more Smart Citation
“…Thus the use of strains containing mutations in PNPase (pnp), RNase II (rnb) and RNase E (rne) has been very helpful for studying mRNA degradation. In particular, SK5704 (pnp-7 rnb-500 rne-1) and its derivatives have been employed extensively to examine the decay of several monocistronic and portions of polycistronic transcripts (Alifano et al, 1994;Arraiano et al, 1988Arraiano et al, , 1993Hajnsdorf et al, 1994;Henry et al, 1992;Mackie, 1991). In this genetic background, upon shift to elevated temperatures, the strain becomes de®cient in PNPase, RNase II and RNase E and, for many mRNAs, decay intermediates are stabilized.…”
Section: Pyrf-orff Transcript Decaymentioning
confidence: 99%
“…In addition, Arraiano et al (1993) were able to demonstrate that the trxA mRNA was degraded by a series of speci®c endonucleolytic cleavages. Other workers have also taken advan-tage of this approach to map decay sites in a variety of mRNAs (Alifano et al, 1994;Hajnsdorf et al, 1994;Henry et al, 1992;Mackie, 1991).…”
Section: Introductionmentioning
confidence: 98%
“…Interestingly, two ORFs encoding ribosomal proteins were also identified as being up- While upregulation is normally due to increased transcriptional activity, either downregulated ORFs may be transcribed at lower rates or the specific mRNAs may undergo more rapid degradation. For E. coli, the downregulation is not due to an increased decay of non-heat shock mRNAs (7). Although the heat shock response has been studied for many years, little has been reported about repressed genes.…”
mentioning
confidence: 99%
“…In Escherichia coli, at least two minor transcription factors, sigma 32 and sigma E, activated upon cellular stress govern two heat shock gene regulons characterized by distinctive responsive sequences (10)(11)(12)35). mRNA coding for hsps appear to be preferentially translated, while synthesis of non-hsps is suppressed (15). The heat shock response is a universal phenomenon by which cells adapt to thermal stress and to a variety of other environmental stresses (22).…”
mentioning
confidence: 99%