Role of the host cell cycle in theAgrobacterium-mediated genetic transformation ofPetunia: Evidence of an S-phase control mechanism for T-DNA transfer

Divide and conquer: development and cell cycle genes in plant transformation

“…Several studies have shown that the phase of the plant cell cycle at the time of transformation is a major determinant of transformation and regeneration efficiency. Transformation frequency is positively associated with cell division or dedifferentiation, with higher transformation observed in cells with nuclei at the S and G2 phases of the cycle [8,9,29]. Using flow cytometry, Villemont et al [29] found that the highest stable transgene expression in Petunia hybrida takes place when cells are at S phase during transformation with Agrobacterium.…”

“…Transformation frequency is positively associated with cell division or dedifferentiation, with higher transformation observed in cells with nuclei at the S and G2 phases of the cycle [8,9,29]. Using flow cytometry, Villemont et al [29] found that the highest stable transgene expression in Petunia hybrida takes place when cells are at S phase during transformation with Agrobacterium. No transformation occurred if cells were at G0-G1 (quiescent phase), and only transient transgene expression occurred when cells were at M phase [29].…”

“…Using flow cytometry, Villemont et al [29] found that the highest stable transgene expression in Petunia hybrida takes place when cells are at S phase during transformation with Agrobacterium. No transformation occurred if cells were at G0-G1 (quiescent phase), and only transient transgene expression occurred when cells were at M phase [29]. Flow cytometric studies in citrus showed that a high frequency of actively dividing cells in S phase resulted in high rates of stable integration of DNA when co-cultivated with Agrobacterium in a medium rich in auxins [9].…”

Stimulation of the cell cycle and maize transformation by disruption of the plant retinoblastoma pathway

“…In addition, transformation of synchronized tobacco protoplasts during S-M phase results in increased recovery of selection-resistant colonies (20,22) and in higher copy, more complex transgene integrations (23) when compared with nonsynchronized cells. More recently, Agrobacterium-mediated delivery of DNA was found to require transition through S phase (24). Although cell cycle dynamics can influence transformation, no practical methods have been reported to positively influence transformation by directly manipulating the cell cycle.…”

<i>Agrobacterium</i>-Mediated Transformation in Liliaceous Ornamental Plants

“…orientalis and M. armeniacum, transgenic plants were produced via transformation of embryogenic calli, whereas our preliminary experiments indicated that neither transformed tissues nor plants could be obtained from any organized tissues. Embryogenic calli have generally been found to have high proliferation and regeneration abilities 25 and have been successfully utilized as a target material for Agrobacterium-mediated production of transgenic plants in both monocotyledonous 3 and dicotyledonous plant species 13 . For L. formosanum, no transgenic plants could be obtained following co-cultivation of organogenic calli with Agrobacterium, which may partly be due to the apparently slower growth of these calli than that of the embryogenic calli from the other 2 species.…”