Role of the insulin-like growth factor system in gonad sexual maturation in Pacific oyster Crassostrea gigas

Moon, Ji‐Sung; Choi, Youn‐Hee

doi:10.1186/s41240-020-00152-z

Cited by 6 publications

(3 citation statements)

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“…FxRIamide, GGNG and insulin-like peptide genes were found at relatively high levels in the mantle, while the foot contained a notable level of myomodulin. In molluscs, the insulin-like peptide has been studies in different oysters ( Crasstostera gigas and Pinctada fucata martensii ) where it has been associated with a role in controlling development, growth, reproduction (Moon and Choi 2020 ) and involved in cell activity, glycogen metabolism and other physiological processes (Zhang and He 2020 ; Antonova et al 2012 ). Pedal peptides are known to be involved in the control of locomotion and ciliated activity in molluscs (Hall and Lloyd 1990 ).…”

Section: Resultsmentioning

confidence: 99%

Development and Interrogation of a Transcriptomic Resource for the Giant Triton Snail (Charonia tritonis)

et al. 2021

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Gastropod molluscs are among the most abundant species that inhabit coral reef ecosystems. Many are specialist predators, along with the giant triton snail Charonia tritonis (Linnaeus, 1758) whose diet consists of Acanthaster planci (crown-of-thorns starfish), a corallivore known to consume enormous quantities of reef-building coral. C. tritonis are considered vulnerable due to overexploitation, and a decline in their populations is believed to have contributed to recurring A. planci population outbreaks. Aquaculture is considered one approach that could help restore natural populations of C. tritonis and mitigate coral loss; however, numerous questions remain unanswered regarding their life cycle, including the molecular factors that regulate their reproduction and development. In this study, we have established a reference C. tritonis transcriptome derived from developmental stages (embryo and veliger) and adult tissues. This was used to identify genes associated with cell signalling, such as neuropeptides and G protein-coupled receptors (GPCRs), involved in endocrine and olfactory signalling. A comparison of developmental stages showed that several neuropeptide precursors are exclusively expressed in post-hatch veligers and functional analysis found that FFamide stimulated a significant (20.3%) increase in larval heart rate. GPCRs unique to veligers, and a diversity of rhodopsin-like GPCRs located within adult cephalic tentacles, all represent candidate olfactory receptors. In addition, the cytochrome P450 superfamily, which participates in the biosynthesis and degradation of steroid hormones and lipids, was also found to be expanded with at least 91 genes annotated, mostly in gill tissue. These findings further progress our understanding of C. tritonis with possible application in developing aquaculture methods.

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Section: Resultsmentioning

confidence: 99%

Development and Interrogation of a Transcriptomic Resource for the Giant Triton Snail (Charonia tritonis)

et al. 2021

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“…Growth hormone (GH) is the activator for the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway (Bergan-Roller & Sheridan, 2018). IGF1 has been linked to growth, Frontiers in Genetics frontiersin.org metabolism (Castillo et al, 2004), development (Moon & Choi, 2020), reproduction (Reinecke, 2010), and osmoregulation (Chandhini et al, 2021) in fish and shellfish. The IGF-binding proteins promote IGF binding to the IGF receptor.…”

Section: Gh-igf System Pi3k/akt and Mtor Pathways Hint Elevated Prote...mentioning

confidence: 99%

Identification of differentially expressed genes and SNPs linked to harvest body weight of genetically improved rohu carp, Labeo rohita

Nandanpawar

Sahoo

et al. 2023

Front. Genet.

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In most of the aquaculture selection programs, harvest body weight has been a preferred performance trait for improvement. Molecular interplay of genes linked to higher body weight is not elucidated in major carp species. The genetically improved rohu carp with 18% average genetic gain per generation with respect to harvest body weight is a promising candidate for studying genes’ underlying performance traits. In the present study, muscle transcriptome sequencing of two groups of individuals, with significant difference in breeding value, belonging to the tenth generation of rohu carp was performed using the Illumina HiSeq 2000 platform. A total of 178 million paired-end raw reads were generated to give rise to 173 million reads after quality control and trimming. The genome-guided transcriptome assembly and differential gene expression produced 11,86,119 transcripts and 451 upregulated and 181 downregulated differentially expressed genes (DEGs) between high-breeding value and low-breeding value (HB & LB) groups, respectively. Similarly, 39,158 high-quality coding SNPs were identified with the Ts/Tv ratio of 1.23. Out of a total of 17 qPCR-validated transcripts, eight were associated with cellular growth and proliferation and harbored 13 SNPs. The gene expression pattern was observed to be positively correlated with RNA-seq data for genes such as myogenic factor 6, titin isoform X11, IGF-1 like, acetyl-CoA, and thyroid receptor hormone beta. A total of 26 miRNA target interactions were also identified to be associated with significant DETs (p-value < 0.05). Genes such as Myo6, IGF-1-like, and acetyl-CoA linked to higher harvest body weight may serve as candidate genes in marker-assisted breeding and SNP array construction for genome-wide association studies and genomic selection.

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“…Marine ILPs have been documented in molluscs; in the Californian sea hare Aplysia californica ILPs have a role in metabolism [ 10 ]. In the Pacific oyster ( Crassostrea gigas ), ILPs are involved in sexual maturation [ 11 ]. ILPs have also been identified in the purple sea urchin ( Strongylocentrotus purpratus ) and the Eastern rock lobster ( Sagmariasus verreauxi ) [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Identification, Synthesis, Conformation and Activity of an Insulin-like Peptide from a Sea Anemone

et al. 2021

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The role of insulin and insulin-like peptides (ILPs) in vertebrate animals is well studied. Numerous ILPs are also found in invertebrates, although there is uncertainty as to the function and role of many of these peptides. We have identified transcripts with similarity to the insulin family in the tentacle transcriptomes of the sea anemone Oulactis sp. (Actiniaria: Actiniidae). The translated transcripts showed that these insulin-like peptides have highly conserved A- and B-chains among individuals of this species, as well as other Anthozoa. An Oulactis sp. ILP sequence (IlO1_i1) was synthesized using Fmoc solid-phase peptide synthesis of the individual chains, followed by regioselective disulfide bond formation of the intra-A and two interchain disulfide bonds. Bioactivity studies of IlO1_i1 were conducted on human insulin and insulin-like growth factor receptors, and on voltage-gated potassium, sodium, and calcium channels. IlO1_i1 did not bind to the insulin or insulin-like growth factor receptors, but showed weak activity against KV1.2, 1.3, 3.1, and 11.1 (hERG) channels, as well as NaV1.4 channels. Further functional studies are required to determine the role of this peptide in the sea anemone.

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Role of the insulin-like growth factor system in gonad sexual maturation in Pacific oyster Crassostrea gigas

Cited by 6 publications

References 50 publications

Development and Interrogation of a Transcriptomic Resource for the Giant Triton Snail (Charonia tritonis)

Development and Interrogation of a Transcriptomic Resource for the Giant Triton Snail (Charonia tritonis)

Identification of differentially expressed genes and SNPs linked to harvest body weight of genetically improved rohu carp, Labeo rohita

Identification, Synthesis, Conformation and Activity of an Insulin-like Peptide from a Sea Anemone

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