Role of the Interaction of Tumor Necrosis Factor-α and Tumor Necrosis Factor Receptors 1 and 2 in Bone-Related Cells

Kitaura, Hideki; Marahleh, Aseel; Ohori, Fumitoshi; Noguchi, Takahiro; Nara, Yasuhiko; Pramusita, Adya; Kinjo, Ria; Ma, Jinghan; Kanou, Kayoko; Mizoguchi, Itaru

doi:10.3390/ijms23031481

Cited by 43 publications

(21 citation statements)

References 194 publications

(247 reference statements)

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“…The overall RSD value of 2.4% obtained for the current signals measured for the individual mixing of other cytokines with TNF-α and all together, with a maximum relative signal variation of 5%, confirmed that the selective detection of TNF-α cytokine could be achieved in the multicytokine sample matrix (Figure Biv). These findings were in agreement with early reports on cytokine–receptor interactions. , …”

Section: Resultssupporting

confidence: 94%

“…These findings were in agreement with early reports on cytokine−receptor interactions. 33,34 The use of the specific detection antibody and standard HRP-TMB system enhanced the sensitivity of the biomembrane sensor. The analytical performance of the cell membrane-based bioelectronic chip for the sensitive detection of various TNF-α concentrations in the presence of IL-6 cytokine was also studied.…”

Section: Analytical Performance Of the Hybrid Cellmentioning

confidence: 99%

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Using Cell Membranes as Recognition Layers to Construct Ultrasensitive and Selective Bioelectronic Affinity Sensors

et al. 2022

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Conventional sandwich immunosensors rely on antibody recognition layers to selectively capture and detect target antigen analytes. However, the fabrication of these traditional affinity sensors is typically associated with lengthy and multistep surface modifications of electrodes and faces the challenge of nonspecific adsorption from complex sample matrices. Here, we report on a unique design of bioelectronic affinity sensors by using natural cell membranes as recognition layers for protein detection and prevention of biofouling. Specifically, we employ the human macrophage (MΦ) membrane together with the human red blood cell (RBC) membrane to coat electrochemical transducers through a one-step process. The natural protein receptors on the MΦ membrane are used to capture target antigens, while the RBC membrane effectively prevents nonspecific surface binding. In an attempt to detect tumor necrosis factor alpha (TNF-α) cytokine using the bioelectronic affinity sensor, it demonstrates a remarkable limit of detection of 150 pM. This new sensor design integrates natural cell membranes and electronic transduction, which offers synergistic functionalities toward a broad range of biosensing applications.

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Section: Resultssupporting

confidence: 94%

Section: Analytical Performance Of the Hybrid Cellmentioning

confidence: 99%

Using Cell Membranes as Recognition Layers to Construct Ultrasensitive and Selective Bioelectronic Affinity Sensors

et al. 2022

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“…For example, a pro-inflammatory cytokine, TNF-alpha, induced the sclerostin expression in osteoblast and osteocyte cells ( 53 – 55 ). Moreover, TNF-alpha contributes to osteoporosis, promoting RANKL-induced osteoclast formation ( 56 , 57 ). In GD, the elevation of TNF-alpha is associated with macrophage and T-cell activations ( 58 – 60 ).…”

Section: Discussionmentioning

confidence: 99%

Wnt signaling pathway inhibitors, sclerostin and DKK-1, correlate with pain and bone pathology in patients with Gaucher disease

et al. 2022

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Patients with Gaucher disease (GD) have progressive bone involvement that clinically presents with debilitating bone pain, structural bone changes, bone marrow infiltration (BMI), Erlenmeyer (EM) flask deformity, and osteoporosis. Pain is referred by the majority of GD patients and continues to persist despite the type of therapy. The pain in GD is described as chronic deep penetrating pain; however, sometimes, patients experience severe acute pain. The source of bone pain is mainly debated as nociceptive pain secondary to bone pathology or neuropathic or inflammatory origins. Osteocytes constitute a significant source of secreted molecules that coordinate bone remodeling. Osteocyte markers, sclerostin (SOST) and Dickkopf-1 (DKK-1), inactivate the canonical Wnt signaling pathway and lead to the inhibition of bone formation. Thus, circulated sclerostin and DKK-1 are potential biomarkers of skeletal abnormalities. This study aimed to assess the circulating levels of sclerostin and DKK-1 in patients with GD and their correlation with clinical bone pathology parameters: pain, bone mineral density (BMD), and EM deformity. Thirty-nine patients with GD were classified into cohorts based on the presence and severity of bone manifestations. The serum levels of sclerostin and DKK-1 were quantified by enzyme-linked immunosorbent assays. The highest level of sclerostin was measured in GD patients with pain, BMI, and EM deformity. The multiparameter analysis demonstrated that 95% of GD patients with pain, BMI, and EM deformity had increased levels of sclerostin. The majority of patients with elevated sclerostin also have osteopenia or osteoporosis. Moreover, circulating sclerostin level increase with age, and GD patients have elevated sclerostin levels when compared with healthy control from the same age group. Pearson’s linear correlation analysis showed a positive correlation between serum DKK-1 and sclerostin in healthy controls and GD patients with normal bone mineral density. However, the balance between sclerostin and DKK-1 waned in GD patients with osteopenia or osteoporosis. In conclusion, the osteocyte marker, sclerostin, when elevated, is associated with bone pain, BMI, and EM flask deformity in GD patients. The altered sclerostin/DKK-1 ratio correlates with the reduction of bone mineral density. These data confirm that the Wnt signaling pathway plays a role in GD-associated bone disease. Sclerostin and bone pain could be used as biomarkers to assess patients with a high risk of BMI and EM flask deformities.

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“…One of the ways to investigate the anti‐inflammatory effect of MSCs on HCC xenografts is by determining the expression of various inflammatory mediators such as IL‐1α, IL‐2, IL‐4, IL‐8, IL‐10, TNF‐α and transforming growth factor‐β (TGF‐β) . In this study, we chose to investigate the expression of IL‐1α, IL‐10 and TNF‐α, due to their key role in immune regulation, inflammatory responses, tissue remodelling, cell motility, cell cycle and apoptosis (Malik & Kanneganti, 2018 and Kitaura et al., 2022).…”

Section: Discussionmentioning

confidence: 99%

Anti‐inflammatory effect of mesenchymal stem cells on hepatocellular carcinoma in the xenograft mice model

Hajighasemlou

Nikbakht

Pakzad

et al. 2022

Veterinary Medicine & Sci

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Background: Hepatocellular carcinoma (HCC) is the fifth most diagnosed cancer and the second leading cause of cancer-related deaths worldwide. Sorafenib is the standard treatment used in the advanced stages of HCC. Cell therapy with mesenchymal stem cells (MSCs)-based cell therapy has proven effective in immune regulation and tumour growth inhibition. Objectives: In this study, we investigated the anti-inflammatory effect of MSCs on HCC xenografts. Methods: Human HepG2 cell lines were subcutaneously implanted into the flank of 12 nude mice, divided into three groups: the control group, the IV group (intravenous MSCs injection) and the local group (local MSCs injection). Mice were sacrificed 6 weeks after tumour implantation, and tumours were resected entirety. Quantitative real-time polymerase chain reaction (qRT-PCR) measured the gene expression of inflammatory markers, including tumour necrosis factor-α (TNF-α), interleukin (IL)-1αand IL-10. Aspartate transaminase (AST), alanine transaminase (ALT) and urea levels were measured using spectrophotometry to ensure the safety of MSC therapy.Results: Gene expressions for all three inflammatory markers were reduced in both MSCs groups compared to the control group. AST, ALT and urea levels remained in normal ranges.Conclusions: MSC therapy can reduce inflammation in HCC xenograft mouse models.

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Role of the Interaction of Tumor Necrosis Factor-α and Tumor Necrosis Factor Receptors 1 and 2 in Bone-Related Cells

Cited by 43 publications

References 194 publications

Using Cell Membranes as Recognition Layers to Construct Ultrasensitive and Selective Bioelectronic Affinity Sensors

Using Cell Membranes as Recognition Layers to Construct Ultrasensitive and Selective Bioelectronic Affinity Sensors

Wnt signaling pathway inhibitors, sclerostin and DKK-1, correlate with pain and bone pathology in patients with Gaucher disease

Anti‐inflammatory effect of mesenchymal stem cells on hepatocellular carcinoma in the xenograft mice model

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