Role of the microbiome and its metabolites in ankylosing spondylitis

Song, Ziyi; Yuan, Duo; Zhang, Sheng‐Xiao

doi:10.3389/fimmu.2022.1010572

Cited by 30 publications

(25 citation statements)

References 217 publications

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“…Studies have found that AS is closely related to gut inflammation, and approximately 70% of AS patients have subclinical gut inflammation ( 33 ), which suggests that gut dysbiosis may contribute to this phenomenon. In recent years, many studies have shown that the occurrence of AS is influenced by the gut microbiota ( 1 , 34 ). The gut microbiota mainly consists of bacteria, archaea, viruses, fungi and parasites.…”

Section: Discussionmentioning

confidence: 99%

Alterations in the gut virome in patients with ankylosing spondylitis

Zhang

Yan

et al. 2023

Front. Immunol.

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IntroductionAnkylosing spondylitis (AS), a chronic autoimmune disease, has been linked to the gut bacteriome.MethodsTo investigate the characteristics of the gut virome in AS, we profiled the gut viral community of 193 AS patients and 59 healthy subjects based on a metagenome-wide analysis of fecal metagenomes from two publicly available datasets.ResultsAS patients revealed a significant decrease in gut viral richness and a considerable alteration of the overall viral structure. At the family level, AS patients had an increased abundance of Gratiaviridae and Quimbyviridae and a decreased abundance of Drexlerviridae and Schitoviridae. We identified 1,004 differentially abundant viral operational taxonomic units (vOTUs) between patients and controls, including a higher proportion of AS-enriched Myoviridae viruses and control-enriched Siphoviridae viruses. Moreover, the AS-enriched vOTUs were more likely to infect bacteria such as Flavonifractor, Achromobacter, and Eggerthellaceae, whereas the control-enriched vOTUs were more likely to be Blautia, Ruminococcus, Collinsella, Prevotella, and Faecalibacterium bacteriophages. Additionally, some viral functional orthologs differed significantly in frequency between the AS-enriched and control-enriched vOTUs, suggesting the functional role of these AS-associated viruses. Moreover, we trained classification models based on gut viral signatures to discriminate AS patients from healthy controls, with an optimal area under the receiver operator characteristic curve (AUC) up to 0.936, suggesting the clinical potential of the gut virome for diagnosing AS.DiscussionThis work provides novel insight into the AS gut virome, and the findings may guide future mechanistic and therapeutic studies for other autoimmune diseases.

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Section: Discussionmentioning

confidence: 99%

Alterations in the gut virome in patients with ankylosing spondylitis

Zhang

Yan

et al. 2023

Front. Immunol.

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“…Its root cause of the “three high” lies in our insufficient understanding of the occurrence and development of AS. Although the “arthritogenic peptide” theory has been proposed on the mechanism of HLA-B27 induced AS in recent years ( 2 ), and some scholars have also proposed that AS and HLA-B27 may be related to deficiency gut immunity ( 3 ), however, the exact pathogenesis of AS remains unclear, so far it has not been effectively treated. Therefore, it is still necessary to conduct in-depth research on the pathogenesis of AS, open new directions for the development of more effective therapeutic drugs and promote the final victory over AS.…”

Section: Introductionmentioning

confidence: 99%

Peptidomics analysis of plasma in patients with ankylosing spondylitis

Guo-ning

Xu²,

Jin³

2023

Front. Immunol.

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BackgroundThis study aimed to explore the differential expression of peptides associated with ankylosing spondylitis (AS) patients, enabling identification of potential functional peptides to provide the basis for the novel intervention targets for AS.Material and Methods3 AS patients and 3 healthy volunteers were enrolled in this study. The expression profiles for peptides present in the plasma of AS patients and the healthy individual were analyzed by liquid chromatography-tandem mass spectrometry (LC‐MS/MS). The physicochemical properties and biological functions of identified peptides were further analyzed by bioinformatics. The results of peptide identification were verified by cell viability analysis, using CCK8 and Edu staining assay, and the differential peptides relevant to the disease were screened.Results52 differential peptides were successfully identified using mass spectrometry. 44 peptides were up-regulated, while eight were down-regulated. FGA-peptide (sequences: DSGEGDFLAEGGGVRGPR), C4A-peptide (sequences: NGFKSHAL), and TUBB-peptide (sequences: ISEQFTAMFR) were screened out that could significantly promote the proliferation of fibroblasts in AS patients. Bioinformatics analysis showed these differentially expressed peptides might be associated with “MHC class I protein binding” and “pathogenic Escherichia coli infection” pathways, which might further affect the progression of AS.ConclusionThis pilot study shows 3 differentially expressed peptides may have the potential function for the occurrence and development of AS, may provide novel insights into the underlying molecular mechanisms of AS based on peptide omics.

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“…Gut microbiota regulate bone growth, gastrointestinal permeability, and inflammation through various mechanisms [ 7 , 8 ]. Among the potential mechanisms, increased permeability between intestinal epithelial cells (IECs) contributes to the progression of AS [ 9 ]. In addition, gut microbiota promote new bone formation in AS by regulating the balance of helper T cells (Th17) and regulatory T cells (Treg) in the lamina propria (LP) to reduce the Th17/Treg ratio [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Dietary Fiber Intake Influences Changes in Ankylosing Spondylitis Disease Status

Song

Wang

et al. 2023

JCM

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The objectives of this study were to characterize dietary fiber (DF) intake in patients with ankylosing spondylitis (AS), to assess whether DF intake affects disease activity in AS, and to investigate the effect of DF intake on disease activity in AS in the context of functional bowel disease (FBD) symptoms. We recruited 165 patients with AS and divided them into two groups according to whether they had a high DF intake > 25 g/d to investigate the characteristics of people with high DF intake. Some 72 of the 165 AS patients (43%) met the criteria for high DF intake, which was more common in patients with negative FBD symptoms (68%). Data analysis revealed that DF intake was negatively associated with AS disease activity and did not differ statistically significantly from FBD symptoms. Multivariate adjusted models were used to explore the effect of DF intake on AS disease activity. ASDAS-CRP and BASDAI were stable and negatively correlated across models in both groups with and without FBD symptoms. Thus, DF intake positively affected disease activity in patients with AS. ASDAS-CRP and BASDAI were negatively correlated with DF intake.

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Role of the microbiome and its metabolites in ankylosing spondylitis

Cited by 30 publications

References 217 publications

Alterations in the gut virome in patients with ankylosing spondylitis

Alterations in the gut virome in patients with ankylosing spondylitis

Peptidomics analysis of plasma in patients with ankylosing spondylitis

Dietary Fiber Intake Influences Changes in Ankylosing Spondylitis Disease Status

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