Role of the oviduct and oviduct-derived products in ruminant embryo development

Rizos, Dimitrios; Maillo, V.; Lonergan, P.

doi:10.21451/1984-3143-ar863

Cited by 11 publications

(6 citation statements)

References 92 publications

(46 reference statements)

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“…Through Panther classification system and GO, glycodelin was identified to have catalytic activity and is associated with a role in cellular and metabolic processes. Studies have shown that glycodelin is synthesized in the endometrium (Seppälä et al, 2002;Spencer et al, 2004) as well as the luteinized granulosa cells of the ovary and is present in follicular fluid (Glister et al, 2014;Rizos et al, 2016). The timely expression of glycodelin during reproduction, in particular fertilization and implantation, is regulated by the endocrine system (Seppälä et al, 1997;Taheripanah et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Proteome profiling of exosomes derived from plasma of heifers with divergent genetic merit for fertility

Koh

Peiris

Vaswani

et al. 2018

Journal of Dairy Science

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The current study evaluated exosomes isolated from plasma of heifers bred to have high or low fertility through developing extreme diversity in fertility breeding values, however, key animal traits (e.g., body weight, milk production, and percentage of North American genetics) remained similar between the 2 groups. The exosomes were isolated by a combined ultracentrifugation and size exclusion chromatography approach and characterized by their size distribution (nanoparticle tracking analysis), morphology (transmission electron microscopy), and presence of exosomal markers (immunoblotting). In addition, a targeted mass spectrometry approach was used to confirm the presence of 2 exosomal markers, tumor susceptibility gene 101 and flotillin 1. The number of exosomes from plasma of high fertility heifers was greater compared with low fertility heifers. Interestingly, the exosomal proteomic profile, evaluated using mass spectrometry, identified 89 and 116 proteins in the high and low fertility heifers respectively, of which 4 and 31 were unique, respectively. These include proteins associated with specific biological processes and molecular functions of fertility. Most notably, the tetratricopeptide repeat protein 41-related, glycodelin, and kelch-like protein 8 were identified in plasma exosomes unique to the low fertility heifers. These proteins are suggested to play a role in reproduction; however, the role of these proteins in dairy cow reproduction remains to be elucidated. Their identification underscores the potential for proteins within exosomes to provide information on the fertility status and physiological condition of the cow. This may potentially lead to the development of prognostic tools and interventions to improving dairy cow fertility.

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Section: Discussionmentioning

confidence: 99%

Proteome profiling of exosomes derived from plasma of heifers with divergent genetic merit for fertility

Koh

Peiris

Vaswani

et al. 2018

Journal of Dairy Science

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“…Mammalian oocytes are fertilized in the oviduct and are developed while traveling inside the fallopian tube toward the uterus. Oviduct fluid is the environment first experienced by oocytes and actively regulates fertilization and subsequent preimplantation development (Rizos et al 2016, Pérez-Cerezales et al 2017. Chemical composition of oviduct fluids contains a variety of molecules including simple and complex carbohydrates, inorganic ions, lipids, phospholipids lactate, pyruvate, glucose, proteins and free amino acids (Leese et al 2001, Avilés et al 2010, Rizos et al 2016, Pérez-Cerezales et al 2017.…”

Section: Introductionmentioning

confidence: 99%

“…Oviduct fluid is the environment first experienced by oocytes and actively regulates fertilization and subsequent preimplantation development (Rizos et al 2016, Pérez-Cerezales et al 2017. Chemical composition of oviduct fluids contains a variety of molecules including simple and complex carbohydrates, inorganic ions, lipids, phospholipids lactate, pyruvate, glucose, proteins and free amino acids (Leese et al 2001, Avilés et al 2010, Rizos et al 2016, Pérez-Cerezales et al 2017. Among the amino acids found in oviduct fluid, the non-essential amino acid glycine is one of the most abundant amino acids reaching up to few millimolar levels in many mammalian species, including bovine (Hugentobler et al 2007), rabbits (Leese et al 1979, Miller & Schultz 1987, and rats (Nakamura et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Glycine receptor α4 subunit facilitates the early embryonic development in mice

et al. 2020

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Oviduct fluid is essential for the fertilization and subsequent preimplantation development. Glycine is abundant in oviduct fluid and is reported to be critical for preimplantation development of fertilized eggs in mammals. However, the mechanism by which glycine exerts its action on fertilized eggs is yet to be understood. Here we show that glycine regulates the preimplantation development of mouse fertilized eggs via glycine receptors. Among them, the alpha-4 subunit (Glra4) and the β subunit are expressed in mouse fertilized eggs, and lacking Glra4 inhibits embryonic development to the blastocyst stage, decreases the number of cells in the blastocysts and the litter size. Thus, we identify a novel function of the glycine receptor, which is considered to act mainly as a neurotransmitter receptor, as a regulator of embryonic development and our data provide new insights into the interactions between oviduct milieu and mammalian fertilized egg.

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“…Understanding the molecular communication that occurs between the developing conceptus (embryo and associated extraembryonic membranes) and the maternal endometrium is key to enhancing our understanding of early pregnancy loss in cattle. During early pregnancy, the embryo induces very localised changes at the transcriptional level in the oviduct [1,2] and in vitro coculture of embryos with oviductal cells accelerates blastocyst development and changes the epigenetic status of the embryos [2,3]. This indicates that interaction with the female reproductive tract, while not strictly necessary for early embryo development, enhances embryo quality [4].…”

Section: Introductionmentioning

confidence: 99%

Protein Synthesis by Day 16 Bovine Conceptuses during the Time of Maternal Recognition of Pregnancy

Estepa

Tinning

Vasconcelos

et al. 2020

IJMS

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Interferon Tau (IFNT), the conceptus-derived pregnancy recognition signal in cattle, significantly modifies the transcriptome of the endometrium. However, the endometrium also responds to IFNT-independent conceptus-derived products. The aim of this study was to determine what proteins are produced by the bovine conceptus that may facilitate the pregnancy recognition process in cattle. We analysed by mass spectrometry the proteins present in conceptus-conditioned media (CCM) after 6 h culture of Day 16 bovine conceptuses (n = 8) in SILAC media (arginine- and lysine-depleted media supplemented with heavy isotopes) and the protein content of extracellular vesicles (EVs) isolated from uterine luminal fluid (ULF) of Day 16 pregnant (n = 7) and cyclic (n = 6) cross-bred heifers on day 16. In total, 11,122 proteins were identified in the CCM. Of these, 5.95% (662) had peptides with heavy labelled amino acids, i.e., de novo synthesised by the conceptuses. None of these proteins were detected in the EVs isolated from ULF. Pregnancy-associated glycoprotein 11, Trophoblast Kunitz domain protein 1 and DExD-Box Helicase 39A were de novo produced and present in the CCM from all conceptuses and in previously published CCM data following 6 and 24 h. A total of 463 proteins were present in the CCM from all the conceptuses in the present study, and after 6 and 24 h culture in a previous study, while expression of their transcripts was not detected in endometrium indicating that they are likely conceptus-derived. Of the proteins present in the EVs, 67 were uniquely identified in ULF from pregnant heifers; 35 of these had been previously reported in CCM from Day 16 conceptuses. This study has narrowed a set of conceptus-derived proteins that may be involved in EV-mediated IFNT-independent embryo–maternal communication during pregnancy recognition in cattle.

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Role of the oviduct and oviduct-derived products in ruminant embryo development

Cited by 11 publications

References 92 publications

Proteome profiling of exosomes derived from plasma of heifers with divergent genetic merit for fertility

Proteome profiling of exosomes derived from plasma of heifers with divergent genetic merit for fertility

Glycine receptor α4 subunit facilitates the early embryonic development in mice

Protein Synthesis by Day 16 Bovine Conceptuses during the Time of Maternal Recognition of Pregnancy

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