2020
DOI: 10.1002/yea.3503
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Role of the regulatory genes SEF1, VMA1 and SFU1 in riboflavin synthesis in the flavinogenic yeast Candida famata (Candida flareri)

Abstract: Riboflavin or vitamin B 2 is an essential dietary component for humans and animals that is the precursor of flavin coenzymes flavin mononucleotide and flavin adenine dinucleotide involved in numerous enzymatic reactions. The flavinogenic yeast Candida famata overproduces riboflavin under iron starvation; however, regulation of this process is poorly understood. Regulatory gene SEF1 encoding transcription activator has been identified. Its deletion blocks yeast ability to overproduce riboflavin under iron starv… Show more

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Cited by 13 publications
(7 citation statements)
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“…C. famata are able to restore riboflavin oversynthesis in sef1Δ mutant whereas SEF1 promoters from other non-flavinogenic yeasts did not [10,16].…”
Section: Resultsmentioning
confidence: 94%
See 1 more Smart Citation
“…C. famata are able to restore riboflavin oversynthesis in sef1Δ mutant whereas SEF1 promoters from other non-flavinogenic yeasts did not [10,16].…”
Section: Resultsmentioning
confidence: 94%
“…Promoter of LAC4 C. famata was amplified with primers Ko1 068:TTTGGGCCCTATATAGAGACAATAAGA CCAG and Ko1069: CGCGGATCCGTTGAGT ATATATCTATTACTTC from the genomic DNA of C. famata VKMY-9 (All-Russian Collection of Microorganisms, Pushchino, Russia). Fragment was combined by overleap PCR with primers, ApaI/BamHI double digested and cloned into corresponding sites of recipient plasmid already contained SEF1 C. famata and NTC genes [10]. The resulted recombinant plasmid was named pNTC/pLAC4_cf-SEF1_cf (Fig.…”
Section: Methodsmentioning
confidence: 99%
“…Riboflavin is an important precursor of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) (Balasubramaniam et al, 2019;Andreieva et al, 2020). Riboflavin insufficiency manifests as persistent anemia (Shi et al, 2014).…”
Section: Vitamin Bmentioning
confidence: 99%
“…Yeasts are metabolically versatile, low maintenance, easy to cultivate, safe, and have a high specific growth rate and short duplication time [ 11 , 12 ]. Some of the fluorochromes produced by yeasts are as follows: (1) ergosterol, a precursor of vitamin D 2 , is produced by Cystofilobasidium capitatum , Rhodotorula glutinis , and Sporobolomyces roseus ; (2) β-carotene, a precursor of vitamin A, is produced by the same yeast species mentioned previously ( C. capitatum , R. glutinis , and S. roseus ) [ 9 , 10 , 13 , 14 , 15 ], and (3) riboflavin, an essential vitamin for human nutrition, is produced by some yeast species such as Candida famata and Meyerozyma guilliermoindii (formerly known as Candida or Pichia guilliermondii ) [ 16 , 17 , 18 , 19 , 20 ].…”
Section: Introductionmentioning
confidence: 99%