Role of the sfiA-dependent cell division regulation system in Escherichia coli

Huisman, Olivier; Jacques, M; D’Ari, Richard; Caro, Lucien

doi:10.1128/jb.153.2.1072-1074.1983

Cited by 23 publications

(1 citation statement)

References 24 publications

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“…Cell division, on the other hand, is tightly regulated, even during unperturbed growth. We have investigated possible roles of the sfiA+ function in normal division regulation and have shown in particular that it is not involved in determining cell mass at different growth rates, mass adjustment after a nutritional shift-up, and chromosome segregation during steady-state growth (23). The strains used in these studies were genetically sfiC (unpublished data), so the processes studied must be sfiC-independent as well.…”

Section: Resultsmentioning

confidence: 99%

Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli

D’Ari¹,

Huisman²

1983

J Bacteriol

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Certain Escherichia coli strains were shown to possess a novel system of cell division inhibition, called the SfiC+ phenotype. SfiC+ filamentation had a number of properties similar to those of sfiA-dependent division inhibition previously described: (i) both are associated with the SOS response induced by expression of the recA(Tif) mutation, (ii) both are associated with cell death, (iii) both are amplified in mutants lacking the Lon protease, and (iv) both are suppressed by sfiB mutations. SfiC+ filamentation and sfiA-dependent division inhibition differed in (i) the physiological conditions under which loss of viability is observed, (ii) the extent of amplification in lon mutants, (iii) their genetic regulation (SfiC+ filamentation is not under direct negative control of the LexA repressor), and (iv) their genetic determinants (SfiC+ filamentation depends on a locus, sfiC+, near 28 min on the E. coli map and distinct from sfiA).

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Section: Resultsmentioning

confidence: 99%

Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli

D’Ari¹,

Huisman²

1983

J Bacteriol

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Overview of controls in the Escherichia coli cell cycle

Vinella

D’Ari

1995

BioEssays

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The harmonious growth and cell-to-cell uniformity of steady-state bacterial populations indicate the existence of a well-regulated cell cycle, responding to a set of internal signals. In Escherichia coli, the key events of this cycle are the initiation of DNA replication, nucleoid segregation and the initiation of cell division. The replication initiator is the DnaA protein. In nucleoid segregation, the MukB protein, required for proper partitioning, may be a member of the myosin-kinesin superfamily of mechanoenzymes. In cell division, the FtsZ protein has a tubulin motif, is a GTPase and polymerizes in a ring around midcell during septation; the FtsA protein has an actin-like structure. The nature of the internal signals triggering these events is not known but candidates include cell mass, the superhelical density of the chromosome and the concentration of two regulatory nucleotides, cyclic AMP and ppGpp. The involvement of cytoskeletal-like proteins in key cycle events encourages the notion of a fundamental biological unity in cell cycle regulation in all organisms.

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Regulation of cell division in Escherichia coli: properties of new ftsZ mutants

Belhumeur

Drapeau

1984

Mol Gen Genet

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Cells of Escherichia coli which produce high levels of the sfiA protein are UV-sensitive and filament extensively. It has been postulated that the sfiA protein is a division inhibitor which interacts with the ftsZ protein (formerly called sfiB or sulB) leading to cell division arrest. Under certain conditions, a similar division inhibition is observed with cells harboring a mutationally altered tsM allele, another division gene which was postulated to code for a division inhibitor or a controlling effector thereof (Drapeau et al. (1984). In this communication, we report on the properties of ftsZ mutants isolated under conditions which brought no selective pressure. These mutants have either an increased sensitivity to UV irradiation or filament drastically following a nutritional shift-up, or both, or even cannot grow in a rich medium. They presumably possess a ftsZ protein which responds more readily to the inhibitory action of the wild type sfiA or the mutationally altered tsM1 protein since the phenotypic expressions associated with the mutations are not observed in the presence of the sfiA11 mutation or are amplified when the ftsZ mutant cells harbor the tsM1 allele. These results further support earlier suggestions that sfiA modulates ftsZ activity and establish tsM as an additional regulatory element thereof. In addition, it is shown that E. coli strain B is a naturally occurring ftsZ mutant.

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Role of the sfiA-dependent cell division regulation system in Escherichia coli

Cited by 23 publications

References 24 publications

Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli

Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli

Overview of controls in the Escherichia coli cell cycle

Regulation of cell division in Escherichia coli: properties of new ftsZ mutants

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