Role of the Sigma70 Subunit of Escherichia coli RNA Polymerase in Transcription Activation

Kumar, Ashok; Grimes, Brenda R.; Fujita, Nobuyuki; Makino, Kozo; Malloch, Richard A.; Hayward, Richard S.; Ishihama, Akira

doi:10.1006/jmbi.1994.1001

Cited by 112 publications

(90 citation statements)

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“…The C-terminal portion of 70 (Region 4 plus H5) is involved both in the basic function of RNAP as well as multiple mechanisms that regulate transcription (10,36,40,41) (for review, see Refs. 8,42).…”

Section: Discussionmentioning

confidence: 99%

Bacteriophage T4 MotA Activator and the β-Flap Tip of RNA Polymerase Target the Same Set of σ70 Carboxyl-terminal Residues

Bonocora

Decker

Glass

et al. 2011

Journal of Biological Chemistry

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Background: Transcriptional activators interact with RNA polymerase to redefine gene expression. Results: A phage activator engages a region of the specificity factor of E. coli RNA polymerase, which is normally bound by another portion of RNA polymerase. Conclusion: Using an activator/co-activator system, the phage hijacks the host RNA polymerase. Significance: Small transcriptional factors acting on defined local regions of RNA polymerase can fundamentally change gene expression.

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Section: Discussionmentioning

confidence: 99%

Bacteriophage T4 MotA Activator and the β-Flap Tip of RNA Polymerase Target the Same Set of σ70 Carboxyl-terminal Residues

Bonocora

Decker

Glass

et al. 2011

Journal of Biological Chemistry

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“…We have defined the promoter region of the PmrAregulated genes pmrC, pmrG, pbgPE, and ugd genes. When compared with known promoter consensus sequences (19), they showed weak identity to the E. coli 70 promoter consensus sequence, which is typical for activator-dependent promoters (20,21). PmrA belongs to the OmpR family of response regulators, which are generally transcribed by 70 RNA polymerase (23).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of the PmrA Regulon

Wösten¹,

Groisman

1999

Journal of Biological Chemistry

101

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The two-component system PmrA/PmrB of Salmonella enterica controls expression of several loci including those mediating modifications in the lipopolysaccharide that result in polymyxin resistance. To gain insight in the regulation of polymyxin resistance, we mapped the transcription start sites of the PmrA-regulated genes pmrC, pmrG, pbgPE, and ugd and identified a conserved sequence in the promoter region of the first three genes. His-tagged PmrA protein could gel shift DNA fragments containing the promoters of the pmrC, pmrG, and pbgPE genes but not the udg promoter. DNase I footprinting analysis of the pmrC, pmrG, and pbgPE promoters indicate that phosphorylated as well as unphosphorylated PmrA bind to a 16-base pair imperfect inverted repeat sequence (5-TTAAKTTCTTA-AKGTT-3), which is found 40, 80, and 38 nucleotides upstream from the transcription start sites of the pmrC, pmrG, and pbgPE genes, respectively. Our data suggest that a PmrA dimer activates transcription of the divergent pmrG and pbgPE promoters by binding to a single site in the pmrG-pbgPE intergenic region and that the ugd gene is regulated by the PmrA/PmrB system only indirectly.

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“…The sigma factor determines the promoter specificity of RNAP and also interacts with certain transcriptional activators (9)(10)(11)(12)(13)(14). The elongation of the transcript is undertaken by the core enzyme (a21313').…”

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Transcription activation by phage phi29 protein p4 is mediated by interaction with the alpha subunit of Bacillus subtilis RNA polymerase.

Mencı́a

Monsalve

Rojo

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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We have found that protein p4 can induce the binding of purified B. subtilis a subunit to the late A3 promoter and that this depends on an interaction held between the protein p4 carboxyl-end and the C-terminal region of the a subunit. The use of reconstituted RNAPs with nested deletions at the a subunit carboxyl-end has indicated that the last 15 residues of RNAP a subunit are required for protein p4-dependent transcription activation. The implications of these results for the transcription activation mechanism of protein p4 are discussed. METHODSCloning and Construction of Deletion Mutants ofB. subtilis rpoA Gene, Coding for RNAP a Subunit. Plasmid pT7Ba was obtained by assembling into the pT7-7 expression vector (26) two DNA fragments from plasmids pSA136 and pSW102 (provided by Chester Price, University of California), containing the 5'-and 3'-segments of B. subtilis rpoA gene (27) 6616The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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Role of the Sigma70 Subunit of Escherichia coli RNA Polymerase in Transcription Activation

Cited by 112 publications

References 0 publications

Bacteriophage T4 MotA Activator and the β-Flap Tip of RNA Polymerase Target the Same Set of σ70 Carboxyl-terminal Residues

Bacteriophage T4 MotA Activator and the β-Flap Tip of RNA Polymerase Target the Same Set of σ70 Carboxyl-terminal Residues

Molecular Characterization of the PmrA Regulon

Transcription activation by phage phi29 protein p4 is mediated by interaction with the alpha subunit of Bacillus subtilis RNA polymerase.

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