Role of the Subtilisin-like Serine Protease CJPRB from Cordyceps javanica in Eliciting an Immune Response in Hyphantria cunea

Abstract

Hyphantria cunea is a globally distributed quarantine plant pest. In a previous study, the Cordyceps javanica strain BE01 with a strong pathogenic effect on H. cunea was identified, and overexpression of the subtilisin-like serine protease CJPRB of this strain was found to accelerate the death of H. cunea (previous research results). In this study, the active recombinant CJPRB protein was obtained through the Pichia pastoris expression system. It was found that CJPRB protein administration to H. cunea via infectation, feeding and injection was able to induce changes in protective enzymes, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and polyphenol oxidase (PPO), and the expression of immune defense-related genes in H. cunea. In particular, CJPRB protein injection induced a more rapid, widespread and intense immune response in H. cunea compared to the other two treatment methods. The results suggest that the CJPRB protein may play a role in eliciting a host immune response during infectation by C. javanica.

Metabolite profiling and in-silico studies show multiple effects of insecticidal actinobacterium on Spodoptera littoralis

“…PPO showed a significantly different increase from controls and a decrease from Biosad® 22.8%, while CAT enzymatic activity showed a slightly significant response. Our results indicated that the potency of ES2 against S. littoralis eggs agrees with Wang’s research outcome 26 .…”

“…Polyphenol oxidase (PPO) and catalase (CAT) are two enzymes that play important roles in defense mechanisms. PPO is involved in toxic quinone production from phenolic compounds, while CAT is involved in hydrogen peroxide breakdown 26 . Abdel-Aziz’s research team have proved the role of PPO and CAT in the defense response on Citrus aurantium against Parlatoria ziziphi 27 .…”